Construct: ORF TRCN0000480922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017896.1_s317c1
- Derived from:
- ccsbBroadEn_03800
- DNA Barcode:
- TAACAATTTGGTCCCATTGATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CORO1B (57175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57175 | CORO1B | coronin 1B | NM_001018070.2 | 100% | 100% | |
| 2 | human | 57175 | CORO1B | coronin 1B | NM_020441.3 | 100% | 100% | |
| 3 | mouse | 23789 | Coro1b | coronin, actin binding prot... | NM_011778.2 | 86.5% | 94% | (many diffs) |
| 4 | mouse | 23789 | Coro1b | coronin, actin binding prot... | XM_006531741.2 | 86.5% | 94% | (many diffs) |
| 5 | mouse | 23789 | Coro1b | coronin, actin binding prot... | XM_006531742.1 | 86.5% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1533
- ORF length:
- 1467
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cttccgcaaa gtggtccggc agagcaaatt ccggcatgtg ttcgggcagc 121 cggtcaagaa cgaccagtgc tatgaggaca ttcgcgtgtc ccgtgttacc tgggacagca 181 ccttctgcgc cgtcaacccc aagttcctgg cggtgattgt ggaggccagt ggagggggtg 241 cctttctggt gctcccccta agcaagacgg gccgcattga caaggcctac ccgacggtgt 301 gtgggcacac gggacctgtc ctggacatcg actggtgtcc tcacaacgac gaagtcatag 361 ccagcggctc ggaggactgc acggtcatgg tgtggcagat cccagagaac gggctgacct 421 ccccgctgac agagccggtg gtggtactgg aggggcacac caagcgagtg ggcatcatcg 481 cctggcaccc cacggcccga aacgtgctgc tcagtgcagg ctgcgacaac gtggtactca 541 tctggaatgt gggcacagcg gaggagctgt accgcctgga cagcctgcac cctgacctca 601 tctacaatgt cagctggaac cacaatggca gcctgttttg ctcagcatgc aaggacaaga 661 gcgtgcgcat catcgacccc cgtcggggca ccctggtggc agagcgggag aaggctcatg 721 agggggcccg gcccatgcgg gccatcttcc tggcagatgg caaggtgttc accacaggct 781 tcagccgaat gagcgagcgg cagctggcgc tctgggaccc agaaaacctc gaggaaccca 841 tggccctgca ggaactggac tcgagcaacg gggccctgct gcccttctac gaccccgaca 901 ccagtgtggt ctacgtctgc ggcaagggtg actccagcat ccggtacttt gagatcacag 961 aggagcctcc ctacatccac ttcctgaaca cgttcaccag caaggagccg cagcggggta 1021 tgggcagcat gcccaagcgg ggcctggagg tcagcaagtg cgagatcgcc cggttctaca 1081 aactgcatga gcgcaagtgt gagcccatcg tcatgactgt gccaagaaag tcggaccTCT 1141 TCCAGGATGA TCTGTACCCC GACACAGCCG GGCCCGAGGC AGCCCTGGAG GCTGAGGAGT 1201 GGGTGAGCGG GCGGGATGCC GACCCGATCC TCATCTCACT GCGGGAGGCC TACGTGCCCA 1261 GCAAGCAGCG GGACCTGAAG ATCAGCCGGC GCAACGTGTT GTCTGACAGC CGGCCCGCCA 1321 TGGCCCCGGG CTCCTCCCAC CTAGGGGCCC CCGCCTCCAC CACCACTGCT GCTGATGCCA 1381 CCCCCAGCGG CAGCCTGGCC AGAGCCGGGG AGGCTGGGAA GCTGGAGGAG GTGATGCAGG 1441 AGCTGCGGGC CCTGAGGGCG CTGGTCAAGG AGCAGGGCGA CCGCATCTGC CGCCTGGAGG 1501 AGCAGCTGGG CCGCATGGAG AACGGGGATG CGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1681 ACAATTTGGT CCCATTGATG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt