Construct: ORF TRCN0000480929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013785.1_s317c1
- Derived from:
- ccsbBroadEn_03965
- DNA Barcode:
- CCAGCATTACTCGATATCGGTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCED1A (64773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64773 | PCED1A | PC-esterase domain containi... | NM_022760.6 | 100% | 100% | |
| 2 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260804.2 | 100% | 100% | |
| 3 | human | 64773 | PCED1A | PC-esterase domain containi... | NM_001271168.2 | 88.7% | 88.7% | 202_203ins153 |
| 4 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260805.3 | 88.7% | 88.7% | 202_203ins153 |
| 5 | human | 64773 | PCED1A | PC-esterase domain containi... | XM_005260806.4 | 79.7% | 79.7% | 840_841ins276 |
| 6 | human | 64773 | PCED1A | PC-esterase domain containi... | XR_244222.4 | 56.5% | 1_468del;1061_1062ins247;1584_1726del | |
| 7 | mouse | 319513 | Pced1a | PC-esterase domain containi... | NM_001114541.1 | 86.6% | 85.6% | (many diffs) |
| 8 | mouse | 319513 | Pced1a | PC-esterase domain containi... | NM_178762.4 | 86.6% | 85.6% | (many diffs) |
| 9 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_017318973.1 | 86.6% | 85.6% | (many diffs) |
| 10 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_006499706.3 | 70.5% | 70.9% | (many diffs) |
| 11 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_006499707.3 | 58.5% | 54.7% | (many diffs) |
| 12 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_006499709.3 | 57.6% | 56.7% | (many diffs) |
| 13 | mouse | 319513 | Pced1a | PC-esterase domain containi... | XM_017318975.1 | 55.9% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cttctgtctg tcgagcgagg agccgcgccg cccgctgcga agcgacatgg 121 tccacttcca ggcctcggaa gtccagcagc tgctacacaa caagttcgtg gtcatcttgg 181 gggactccat tcagagggct gtgtacaagg acctggtgct cttgctccag aaagactcac 241 tgctcacagc tgcccagctg aaagccaagg gggagctgag ctttgaacag gaccagctgg 301 tggctggggg ccagctgggc gagctgcaca acgggacaca gtatcgtgag gtccgccagt 361 tctgctcggg ctctggccac caccttgtgc gcttctactt cctcactcgt gtttactccg 421 agtaccttga ggatgttctg gaagagctga catatggacc tgccccggac ctggtgatca 481 tcaactcctg cctctgggat ctctccagat atggtcgctg ctcaatggag agctaccggg 541 agaacctgga gcgggtgttt gtgcgcatgg accaagtatt gccagactcc tgcctgctgg 601 tgtggaacat ggcgatgccc ctcggggaac gtatcactgg gggtttcctc ctgccagagc 661 tccagcccct ggcaggctcc ctgcggcggg atgtggttga agggaacttc tacagtgcta 721 cgctggccgg ggaccactgc tttgatgtcc tagacctcca ctttcacttc cggcatgcag 781 tacagcaccg tcatcgggat ggtgtccact gggaccagca tgcacaccgc cacctctcac 841 acctgcttct gacccatgtg gctgacgcct ggggcgtgga gctgcccaag cgtggctatc 901 cccctgaccc gtggattgag gactgggcag agatgaatca tccattccag ggaagccata 961 ggcagacccc agacttcggg gagcacctgg ccttgctccc acccccacct tcttctttgc 1021 ctcctcccat gccttttccc tacccgcttc cTCAGCCCTC GCCACCTCCC CTCTTCCCAC 1081 CCCTGCCCCA GGATACCCCT TTTTTCCCAG GCCAGCCCTT CCCACCCCAT GAATTCTTCA 1141 ACTATAATCC AGTGGAGGAC TTCTCGATGC CACCCCACTT AGGATGTGGC CCTGGAGTGA 1201 ACTTTGTGCC TGGCCCTCTG CCACCTCCAA TCCCTGGCCC TAATCCCCAT GGTCAGCACT 1261 GGGGCCCAGT GGTCCACCGG GGGATGCCAC GCTATGTTCC TAACAGCCCC TACCATGTGC 1321 GGAGAATGGG GGGGCCCTGC AGGCAGCGGC TCAGACACTC AGAGAGACTG ATCCACACAT 1381 ACAAACTGGA CAGACGGCCT CCTGCCCATT CGGGGACATG GCCTGGGTAC CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGACCAGCAT TACTCGATAT CGGTGCAACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt