Transcript: Mouse XM_017318975.1

PREDICTED: Mus musculus PC-esterase domain containing 1A (Pced1a), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pced1a (319513)
Length:
2481
CDS:
188..1078

Additional Resources:

NCBI RefSeq record:
XM_017318975.1
NBCI Gene record:
Pced1a (319513)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318975.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000190249 CACTGTTTCGACGTCCTAGAT pLKO.1 398 CDS 100% 4.950 6.930 N Pced1a n/a
2 TRCN0000201990 CTTTGTACGAATGGACCAGGT pLKO.1 220 CDS 100% 2.160 3.024 N Pced1a n/a
3 TRCN0000202354 GATTGAGGACTGGTCAGAGAT pLKO.1 577 CDS 100% 4.950 3.960 N Pced1a n/a
4 TRCN0000189985 GCCACGTTGTGTTCTGAACAA pLKO.1 934 CDS 100% 4.950 3.960 N Pced1a n/a
5 TRCN0000129522 GCTGCTACACAACAAGTTCGT pLKO.1 51 5UTR 100% 2.640 1.848 N PCED1A n/a
6 TRCN0000338668 GCTGCTACACAACAAGTTCGT pLKO_005 51 5UTR 100% 2.640 1.848 N PCED1A n/a
7 TRCN0000131185 GCTACACAACAAGTTCGTGGT pLKO.1 54 5UTR 100% 2.160 1.512 N PCED1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318975.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03965 pDONR223 100% 55.9% 54.6% None (many diffs) n/a
2 ccsbBroad304_03965 pLX_304 0% 55.9% 54.6% V5 (many diffs) n/a
3 TRCN0000480929 CCAGCATTACTCGATATCGGTGCA pLX_317 28% 55.9% 54.6% V5 (many diffs) n/a
4 ccsbBroadEn_12488 pDONR223 100% 44.5% 44.1% None (many diffs) n/a
5 ccsbBroad304_12488 pLX_304 0% 44.5% 44.1% V5 (many diffs) n/a
6 TRCN0000471857 TTCTCTGACAGATAGAGAATCCTA pLX_317 42.7% 44.5% 44.1% V5 (many diffs) n/a
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