Construct: ORF TRCN0000480931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012001.1_s317c1
- Derived from:
- ccsbBroadEn_14225
- DNA Barcode:
- TACAAAACACCTACGCGAAAATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHBG (57127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NM_001256395.2 | 86.8% | 82% | (many diffs) |
| 2 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NM_001256396.2 | 79.5% | 75.2% | (many diffs) |
| 3 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NM_020407.5 | 74.7% | 70.7% | (many diffs) |
| 4 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XM_017001857.1 | 72.5% | 72.1% | (many diffs) |
| 5 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XM_017001858.1 | 71.7% | 71.5% | (many diffs) |
| 6 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XM_011509799.2 | 70.2% | 55% | (many diffs) |
| 7 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XM_017001859.1 | 63% | 58.1% | (many diffs) |
| 8 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XM_011509800.2 | 57.9% | 48% | (many diffs) |
| 9 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737330.1 | 57.2% | (many diffs) | |
| 10 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737324.1 | 55.6% | (many diffs) | |
| 11 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737323.1 | 54.9% | (many diffs) | |
| 12 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NR_046115.2 | 54.4% | (many diffs) | |
| 13 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737325.1 | 53.6% | (many diffs) | |
| 14 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737329.1 | 52.4% | (many diffs) | |
| 15 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NR_146764.2 | 51.9% | (many diffs) | |
| 16 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NR_146765.2 | 49.5% | (many diffs) | |
| 17 | human | 57127 | RHBG | Rh family B glycoprotein (g... | XR_001737328.1 | 43.2% | (many diffs) | |
| 18 | human | 57127 | RHBG | Rh family B glycoprotein (g... | NR_146763.2 | 38.8% | (many diffs) | |
| 19 | mouse | 58176 | Rhbg | Rhesus blood group-associat... | NM_021375.3 | 64.9% | 63.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1293
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cttcgtgggc tttggcttcc tcatggtctt cctgcagcgt tacggcttca 121 gcagcgtggg cttcaccttc ctcctggccg cctttgccct gcagtggtcc acactggtcc 181 agggctttct ccactccttc cacggtggcc acatccatgt tggcgtggag agcatgatca 241 atgctgactt ttgtgcgggg gccgtgctca tctcctttgg tgccgacctg ggcaagaccg 301 ggcctaccca gctgctgctc atggccctgc tggaggtggt gctgtttggc atcaatgagt 361 ttgtgctcct tcatctcctg ggggtgagag tctggggagg gatggagtcg ggggtggggg 421 gtggtcaagg tcagcctctg agccaggagc gtgggggtgg gggcggggtg ctgcctctca 481 ccccacctcc ccaggtgaga gatgccggag gctccatgac tatccacacc tttggtgcct 541 acttcgggct cgtcctttcg cgggttctgt acaggcccca gctggagaag agcaagcacc 601 gccagggctc cgtctaccat tcagacctct tcgccatgat tgggaccatc ttcctgtgga 661 tcttctggcc tagcttcaat gctgcactca catcgctggg ggctgggcag catcggacgg 721 ccctcaacac atactactcc ctggctgcca gcacccttgg cacctttgcc ttgtcagccc 781 ttgtagggga agatgggagg cttgacatgg tccacatcca aaatgcagcg ctggctggag 841 gggttgtggt ggggacctca agtgaaatga tgctgacacc ctttgggGCT CTGGCAGCTG 901 GCTTCTTGGC TAGGACTGTC TCCACGCTGG GGTACAAGTT CTTCACGCCC ATCCTTGAAT 961 CAAAATTCAA AGTCCAAGAC ACATGTGGAG TCCACAACCT CCATGGGATG CCGGGGGTCC 1021 TGGGGGCCCT CCTGGGGGTC CTTGTGGCTG GACTTGCCAC CCATGAAGCT TACGGAGATG 1081 GCCTGGAGAG TGTGTTTCCA CTCATAGCCG AGGGCCAGCG CAGTGCCACG TCACAGGCCA 1141 TGCACCAGCT CTTCGGGCTG TTTGTCACAC TGATGTTTGC CTCTGTGGGC GGGGGCCTTG 1201 GAGGGCTCCT GCTGAAGCTA CCCTTTCTGG ACTCCCCCCC CAGACTCCCA GCGCTACGAG 1261 GACCAAGTTC ACTGGCAGGT GCCTGGCGAG CATGCCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1441 CAAAACACCT ACGCGAAAAT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt