Construct: ORF TRCN0000480932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011826.1_s317c1
- Derived from:
- ccsbBroadEn_12160
- DNA Barcode:
- GTTCCCGCACCGGTAAAGCACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM39B (55116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55116 | TMEM39B | transmembrane protein 39B | NM_001319678.2 | 79.8% | 79.8% | 1_177del |
| 2 | human | 55116 | TMEM39B | transmembrane protein 39B | NM_001319679.2 | 79.8% | 79.8% | 1_177del |
| 3 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_011541684.3 | 79.8% | 79.8% | 1_177del |
| 4 | human | 55116 | TMEM39B | transmembrane protein 39B | NM_001319677.1 | 64.1% | 64.1% | 1_393del |
| 5 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_005270988.2 | 64.1% | 64.1% | 1_393del |
| 6 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_011541683.1 | 64.1% | 64.1% | 1_393del |
| 7 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_017001595.1 | 62% | 62% | 1_429del |
| 8 | human | 55116 | TMEM39B | transmembrane protein 39B | NM_018056.4 | 47.5% | 47.5% | 1_774del |
| 9 | human | 55116 | TMEM39B | transmembrane protein 39B | XR_001737262.1 | 44.2% | 1_587del;929_1031del;1393_1588del | |
| 10 | human | 55116 | TMEM39B | transmembrane protein 39B | XR_002956975.1 | 36.5% | 1_920del;1262_1364del;1726_1921del | |
| 11 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_006710724.2 | 34.1% | 32.4% | (many diffs) |
| 12 | human | 55116 | TMEM39B | transmembrane protein 39B | XM_006710725.4 | 33% | 31.5% | (many diffs) |
| 13 | mouse | 230770 | Tmem39b | transmembrane protein 39b | XM_006503046.2 | 59.5% | 63.5% | (many diffs) |
| 14 | mouse | 230770 | Tmem39b | transmembrane protein 39b | XM_011240504.1 | 59.5% | 63.5% | (many diffs) |
| 15 | mouse | 230770 | Tmem39b | transmembrane protein 39b | XM_006503045.2 | 57.6% | 61.5% | (many diffs) |
| 16 | mouse | 230770 | Tmem39b | transmembrane protein 39b | NM_199305.1 | 44.1% | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cacccatgcc tgctgcctgt cacccagcct catccgcagt gaggtggagt 121 tcctcaagat ggacttcaac tggcgcatga aggaagtgct cgtcagctcc atgctgagcg 181 cctactatgt ggcctttgtg cctgtctggt tcgtgaagaa cacacattac tatgacaagc 241 gctggtcctg tgaactcttc ctgctggtgt ccatcagcac ctccgtgatc ctcatgcagc 301 acctgctgcc tgccagctac tgtgaccTGC TGCACAAGGC CGCCGCCCAT CTGGGCTGTT 361 GGCAGAAGGT GGACCCAGCG CTGTGCTCCA ACGTGCTGCA GCACCCGTGG ACTGAAGAAT 421 GCATGTGGCC GCAGGGCGTG CTGGTGAAGC ACAGCAAGAA CGTCTACAAA GCCGTAGGCC 481 ACTACAACGT GGCTATCCCC TCTGACGTCT CCCACTTCCG CTTCCATTTC TTTTTCAGCA 541 AACCCCTGCG GATCCTCAAC ATCCTCCTGC TGCTGGAGGG CGCTGTCATT GTCTATCAGC 601 TGTACTCCCT AATGTCCTCT GAAAAGTGGC ACCAGACCAT CTCGCTGGCC CTCATCCTCT 661 TCAGCAACTA CTATGCCTTC TTCAAGCTGC TCCGGGACCG CTTGGTATTG GGCAAGGCCT 721 ACTCATACTC TGCTAGCCCC CAGAGAGACC TGGACCACCG TTTCTCCTGC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAGTTCCCG CACCGGTAAA GCACCGCACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt