Construct: ORF TRCN0000480956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000554.4_s317c1
- Derived from:
- ccsbBroadEn_15155
- DNA Barcode:
- CCGAAGCATACCGGTGCCTATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEK11 (79858)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480956
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001321223.1 | 100% | 100% | |
| 2 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_145910.4 | 97.3% | 96.6% | (many diffs) |
| 3 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007221.1 | 91.9% | 91.9% | 335_460del |
| 4 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007222.1 | 89.5% | 88.9% | (many diffs) |
| 5 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007220.1 | 89.5% | 89% | (many diffs) |
| 6 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001146003.2 | 79.5% | 78.1% | (many diffs) |
| 7 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353028.2 | 79.5% | 78.1% | (many diffs) |
| 8 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353029.2 | 79.5% | 78.1% | (many diffs) |
| 9 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353026.2 | 74.3% | 73% | (many diffs) |
| 10 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453755.1 | 74.3% | 73% | (many diffs) |
| 11 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001321220.2 | 73.8% | 72.6% | (many diffs) |
| 12 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353023.2 | 73.8% | 72.6% | (many diffs) |
| 13 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_024800.5 | 73.8% | 72.6% | (many diffs) |
| 14 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353024.2 | 73.7% | 72.4% | (many diffs) |
| 15 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353025.2 | 73.7% | 72.4% | (many diffs) |
| 16 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353030.2 | 73.5% | 72.1% | (many diffs) |
| 17 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353031.2 | 73.5% | 72.1% | (many diffs) |
| 18 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001321221.2 | 69.3% | 68.1% | (many diffs) |
| 19 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353022.2 | 69.3% | 68.1% | (many diffs) |
| 20 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007206.1 | 69.3% | 68.1% | (many diffs) |
| 21 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007207.1 | 69.3% | 68.1% | (many diffs) |
| 22 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007208.1 | 69.3% | 68.1% | (many diffs) |
| 23 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007209.1 | 69.3% | 68.1% | (many diffs) |
| 24 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007210.1 | 69.3% | 68.1% | (many diffs) |
| 25 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353036.2 | 68.8% | 67.5% | (many diffs) |
| 26 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353027.2 | 68.3% | 67% | (many diffs) |
| 27 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353044.2 | 62% | 60.6% | (many diffs) |
| 28 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353045.2 | 62% | 60.6% | (many diffs) |
| 29 | human | 79858 | NEK11 | NIMA related kinase 11 | XR_001740271.1 | 59.3% | (many diffs) | |
| 30 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001321222.2 | 57.6% | 56.3% | (many diffs) |
| 31 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353032.2 | 57.6% | 56.3% | (many diffs) |
| 32 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453756.1 | 57.6% | 56.3% | (many diffs) |
| 33 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353033.2 | 56.9% | 55.2% | (many diffs) |
| 34 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353034.2 | 56.9% | 55.2% | (many diffs) |
| 35 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007219.1 | 56.9% | 55.2% | (many diffs) |
| 36 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353048.2 | 54.9% | 53.5% | (many diffs) |
| 37 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007233.2 | 54.9% | 53.5% | (many diffs) |
| 38 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353037.2 | 52% | 50.7% | (many diffs) |
| 39 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453757.1 | 52% | 50.7% | (many diffs) |
| 40 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353038.2 | 51% | 49.6% | (many diffs) |
| 41 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353039.2 | 51% | 49.6% | (many diffs) |
| 42 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353040.2 | 51% | 49.6% | (many diffs) |
| 43 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353041.2 | 51% | 49.6% | (many diffs) |
| 44 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353042.2 | 51% | 49.6% | (many diffs) |
| 45 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353043.2 | 51% | 49.6% | (many diffs) |
| 46 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_011513173.1 | 51% | 49.6% | (many diffs) |
| 47 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_011513174.1 | 51% | 49.6% | (many diffs) |
| 48 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_011513175.1 | 51% | 49.6% | (many diffs) |
| 49 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_017007228.1 | 51% | 49.6% | (many diffs) |
| 50 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453758.1 | 51% | 49.6% | (many diffs) |
| 51 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453759.1 | 51% | 49.6% | (many diffs) |
| 52 | human | 79858 | NEK11 | NIMA related kinase 11 | XM_024453760.1 | 45.7% | 44.4% | (many diffs) |
| 53 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001321224.2 | 45.4% | 44.1% | (many diffs) |
| 54 | human | 79858 | NEK11 | NIMA related kinase 11 | NM_001353046.2 | 45.4% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaaattccaa gaggcagcta agtgtgtgag tggatcaaca gccatttcca 121 cttatccaaa gaccttgatt gcaagaagat acgtgcttca acaaaaactt ggcagtggaa 181 gttttggaac tgtctatctg gtttcagaca agaaagccaa acgaggagag gaattaaagg 241 tacttaagga aatatctgtt ggagaactaa atccaaatga aactgtacag gccaatttgg 301 aagcccaact cctctccaag ctggaccacc cagccattgt caagttccat gcaagttttg 361 tggagcaaga taatttctgc attatcacgg agtactgtga gggccgagat ctggacgata 421 aaattcagga atataaacaa gctggaaaaa tctttccaga aaatcaaata atagaatggt 481 ttatccagct gctgctggga gttgactaca tgcatgagag gaggatactt catcgagact 541 taaagtcaaa gaatgtattt ctgaaaaata atctccttaa aattggagat tttggagttt 601 ctcgacttct aatgggatcc tgtgacctgg ccacaacttt aactggaact ccccattata 661 tgagtcctga ggctctgaaa caccaaggct atgacacaaa gtcggacatc tggtcactgg 721 catgcatttt gtatgagatg tgctgcatga atcatgcatt cgctggctcc aatttcttat 781 ccattgtttt aaaaattgtt gaaggtgaca caccttctct ccctgagaga tatccaaaag 841 aactaaatgc catcatggaa agcatgttga acaagaatcc ttcattaaga ccatctgcta 901 tcgaaatttt aaaaatccct taccttgatg agcagctaca gaacctaatg tgtagatatt 961 cagaaatgac tctggaagac aaaaatttgg attgtcagaa ggaggctgct catataatta 1021 atgccatgca aaaaaggatc caccTGCAGA CTCTGAGGGC ACTGTCAGAA GTACAGAAAA 1081 TGACGCCAAG AGAAAGGATG CGGCTGAGGA AGCTCCAGGC GGCTGATGAG AAAGCCAGGA 1141 AGCTGAAAAA GATTGTGGAA GAAAAATATG AAGAAAATAG CAAACGAATG CAAGAATTGA 1201 GATCTCGGAA CTTTCAGCAG CTGAGTGTTG ATGTACTCCA TGAAAAAACA CATTTAAAAG 1261 GAATGGAAGA AAAGGAGGAG CAACCTGAGG GAAGACTTTC TTGTTCACCC CAGGACGAGG 1321 ATGAAGAGAG GTGGCAAGGC AGGGAAGAGG AATCTGATGA ACCAACTTTA GAGAACCTGC 1381 CTGAGTCTCA GCCTATTCCT TCCATGGACC TCCACGAACT TGAATCAATT GTAGAGGATG 1441 CCACATCTGA CCTTGGATAC CATGGAGACT GTAATCTAAT TTCACTAGAC GAATACTGGA 1501 AAAATGAAAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCG AAGCATACCG GTGCCTATAC 1681 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t