Transcript: Human XM_024453760.1

PREDICTED: Homo sapiens NIMA related kinase 11 (NEK11), transcript variant X20, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NEK11 (79858)
Length:
2183
CDS:
109..1500

Additional Resources:

NCBI RefSeq record:
XM_024453760.1
NBCI Gene record:
NEK11 (79858)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147265 CCAAGGCTATGACACAAAGT pXPR_003 CGG 88 6% 2 0.5569 NEK11 NEK11 75563
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453760.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001965 CCTTACCTTGATGAGCAGCTA pLKO.1 415 CDS 100% 2.640 2.112 N NEK11 n/a
2 TRCN0000196552 GAACCTAATGTGTAGATATTC pLKO.1 438 CDS 100% 13.200 9.240 N NEK11 n/a
3 TRCN0000195420 CACCAAGGCTATGACACAAAG pLKO.1 178 CDS 100% 10.800 7.560 N NEK11 n/a
4 TRCN0000197110 GCCTATGCTTGGAGTCATAAG pLKO.1 1671 3UTR 100% 10.800 7.560 N NEK11 n/a
5 TRCN0000001964 CCATGACTAATAAGGAAGATA pLKO.1 2005 3UTR 100% 5.625 3.938 N NEK11 n/a
6 TRCN0000001962 CCAGAGAAAGAAATCAGGAAT pLKO.1 1066 CDS 100% 4.950 3.465 N NEK11 n/a
7 TRCN0000001963 GCGTTAGAAAGACCAGAGAAA pLKO.1 1054 CDS 100% 4.950 3.465 N NEK11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453760.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492172 ATTCGGGGACAGATACCACACACA pLX_317 11.9% 71.7% 71.6% V5 (not translated due to prior stop codon) 0_1ins546;917A>T n/a
2 TRCN0000492116 ATGACATGCCTCATGTTCAACCCG pLX_317 21.2% 71.6% 71.5% V5 0_1ins546;917A>T;1389_1390insG n/a
3 ccsbBroadEn_12637 pDONR223 100% 45.7% 44.4% None (many diffs) n/a
4 ccsbBroad304_12637 pLX_304 0% 45.7% 44.4% V5 (many diffs) n/a
5 TRCN0000479883 TGAGCAGACTTAGGCATAAAATCC pLX_317 21.7% 45.7% 44.4% V5 (many diffs) n/a
6 ccsbBroadEn_15155 pDONR223 0% 45.7% 44.4% None (many diffs) n/a
7 ccsbBroad304_15155 pLX_304 0% 45.7% 44.4% V5 (many diffs) n/a
8 TRCN0000480956 CCGAAGCATACCGGTGCCTATACC pLX_317 32.6% 45.7% 44.4% V5 (many diffs) n/a
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