Construct: ORF TRCN0000481035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015086.1_s317c1
- Derived from:
- ccsbBroadEn_16159
- DNA Barcode:
- CTCACAAGTATCCACCTTTAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC339010 (339010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | XM_011543795.2 | 99.7% | 99.4% | 608A>G;690T>C;856C>G |
| 2 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | XM_006720358.2 | 98.5% | 97.1% | (many diffs) |
| 3 | human | 102723502 | LOC102723502 | POTE ankyrin domain family ... | XM_006724901.3 | 98.4% | 97.3% | (many diffs) |
| 4 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | XM_011543797.2 | 98.4% | 98.1% | (many diffs) |
| 5 | human | 102723502 | LOC102723502 | POTE ankyrin domain family ... | XM_011546200.2 | 98.3% | 98.4% | (many diffs) |
| 6 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | XM_011543796.2 | 98.2% | 97.9% | (many diffs) |
| 7 | human | 102723502 | LOC102723502 | POTE ankyrin domain family ... | XM_011546199.2 | 98.1% | 98.1% | (many diffs) |
| 8 | human | 100288966 | LOC100288966 | POTE ankyrin domain family ... | XM_006709930.3 | 97% | 93.4% | (many diffs) |
| 9 | human | 317754 | POTED | POTE ankyrin domain family ... | XM_006723997.3 | 97% | 93.4% | (many diffs) |
| 10 | human | 100288966 | LOC100288966 | POTE ankyrin domain family ... | XM_011545656.2 | 96.9% | 94.4% | (many diffs) |
| 11 | human | 317754 | POTED | POTE ankyrin domain family ... | XM_011529550.2 | 96.7% | 94.2% | (many diffs) |
| 12 | human | 100288966 | LOC100288966 | POTE ankyrin domain family ... | XM_011545655.2 | 96.7% | 94.2% | (many diffs) |
| 13 | human | 100287399 | POTEB2 | POTE ankyrin domain family ... | XM_006720354.3 | 88.8% | 87.5% | (many diffs) |
| 14 | human | 100996331 | POTEB | POTE ankyrin domain family ... | XM_006720355.3 | 88.8% | 87.5% | (many diffs) |
| 15 | human | 100287399 | POTEB2 | POTE ankyrin domain family ... | XM_017021853.1 | 88.6% | 88.4% | (many diffs) |
| 16 | human | 100996331 | POTEB | POTE ankyrin domain family ... | XM_017021858.1 | 88.6% | 88.4% | (many diffs) |
| 17 | human | 100287399 | POTEB2 | POTE ankyrin domain family ... | XM_011543793.2 | 88.4% | 88.1% | (many diffs) |
| 18 | human | 100996331 | POTEB | POTE ankyrin domain family ... | XM_011543794.2 | 88.4% | 88.1% | (many diffs) |
| 19 | human | 340441 | POTEA | POTE ankyrin domain family ... | XM_011544516.1 | 70.6% | 62.2% | (many diffs) |
| 20 | human | 404785 | POTEG | POTE ankyrin domain family ... | NM_001005356.3 | 69.9% | 67.1% | (many diffs) |
| 21 | human | 641455 | POTEM | POTE ankyrin domain family ... | NM_001145442.1 | 69.7% | 66.9% | (many diffs) |
| 22 | human | 388468 | POTEC | POTE ankyrin domain family ... | NM_001137671.1 | 68.6% | 67.3% | (many diffs) |
| 23 | human | 23784 | POTEH | POTE ankyrin domain family ... | NM_001136213.1 | 65.3% | 62.7% | (many diffs) |
| 24 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | NM_207355.3 | 65.1% | 64.5% | (many diffs) |
| 25 | human | 102723502 | LOC102723502 | POTE ankyrin domain family ... | XM_006724900.4 | 65% | 64.7% | (many diffs) |
| 26 | human | 100288966 | LOC100288966 | POTE ankyrin domain family ... | NM_001257362.2 | 63.8% | 61.8% | (many diffs) |
| 27 | human | 317754 | POTED | POTE ankyrin domain family ... | NM_174981.3 | 63.8% | 61.8% | (many diffs) |
| 28 | human | 100287399 | POTEB2 | POTE ankyrin domain family ... | NM_001277303.1 | 58.6% | 58.1% | (many diffs) |
| 29 | human | 100996331 | POTEB | POTE ankyrin domain family ... | NM_001277304.1 | 58.6% | 58.1% | (many diffs) |
| 30 | human | 102724631 | POTEB3 | POTE ankyrin domain family ... | NR_110752.1 | 56.7% | (many diffs) | |
| 31 | human | 404785 | POTEG | POTE ankyrin domain family ... | NR_027480.1 | 53.9% | (many diffs) | |
| 32 | human | 100287399 | POTEB2 | POTE ankyrin domain family ... | NR_102390.1 | 51.3% | (many diffs) | |
| 33 | human | 100996331 | POTEB | POTE ankyrin domain family ... | NR_102391.1 | 51.3% | (many diffs) | |
| 34 | human | 340441 | POTEA | POTE ankyrin domain family ... | NM_001005365.2 | 47.4% | 42.1% | (many diffs) |
| 35 | human | 340441 | POTEA | POTE ankyrin domain family ... | XM_024447146.1 | 42.8% | 38% | (many diffs) |
| 36 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_011511216.2 | 37% | 35.8% | (many diffs) |
| 37 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004159.2 | 36.6% | 35.4% | (many diffs) |
| 38 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001277406.2 | 33% | 32% | (many diffs) |
| 39 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_024453046.1 | 33% | 32% | (many diffs) |
| 40 | human | 445582 | POTEE | POTE ankyrin domain family ... | NM_001083538.2 | 32.9% | 32% | (many diffs) |
| 41 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004161.2 | 32.9% | 32% | (many diffs) |
| 42 | human | 728378 | POTEF | POTE ankyrin domain family ... | NM_001099771.2 | 32.8% | 31.8% | (many diffs) |
| 43 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_011511670.2 | 32.7% | 31.7% | (many diffs) |
| 44 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004732.2 | 32.7% | 31.7% | (many diffs) |
| 45 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004833.1 | 32.5% | 31.5% | (many diffs) |
| 46 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001371926.1 | 32.3% | 31.4% | (many diffs) |
| 47 | human | 440915 | POTEKP | POTE ankyrin domain family ... | NR_033885.3 | 31.7% | (many diffs) | |
| 48 | human | 653781 | POTEJ | POTE ankyrin domain family ... | NM_001277083.2 | 30% | 29.1% | (many diffs) |
| 49 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_011511683.2 | 29.8% | 28.8% | (many diffs) |
| 50 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004741.2 | 29.8% | 28.8% | (many diffs) |
| 51 | human | 388468 | POTEC | POTE ankyrin domain family ... | NR_134878.1 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggctgaggtt tgttcaatgc ccgctgcctc tgctgtgaag aagccatttg 121 atctcaggag caagatgggc aagtggtgcc accaccgctt cccctgctgc agggggagcg 181 gcaagagcaa catgggcact tctggagacc acgacgactc ctttatgaag acgctcagga 241 gcaagatggg caagtgttgc caccactgct tcccctgctg cagggggagc ggcacgagca 301 atgtgggcac ttctggagac catgacaact cctttatgaa gacactcagg agcaagatgg 361 gcaagtggtg ctgtcactgc ttcccctgct gcagggggag cggcaagagc aacgtgggca 421 cttggggaga ctacgacgac agcgccttca tggagccgag gtaccacgtc cgtcgagaag 481 atctggacaa gctccacaga gctgcctggt ggggtaaagt ccccagaaag gatctcatcg 541 tcatgctcag ggacactgac atgaacaaga gggacaagca aaagaggact gctctacatt 601 tggcctctgc caatggaaat tcagaagtag tacaactcct gctggacaga cgatgtcaac 661 ttaacgtcct tggcaacaaa aaaaggacag ctctgataaa ggccgtacaa tgccaggaag 721 atgaatgtgt gttaatgttg ctggaacatg GCGCCGATGG AAATATTCAA GATGAGTATG 781 GAAATACCGC TCTACACTAT GCTATCTACA ATGAAGATAA ATTAATGGCC AAAGCACTGC 841 TCTTATATGG TGCTGATATT GAATCAAAAA ACAAGTGTGG CCTCACACCA CTTTTGCTTG 901 GCGTACATGA ACAAAAACAG GAAGTGGTGA AATTTTTAAT CAAGAAAAAA GCTAATTTAA 961 ATGCACTTGA TAGATATGGA AGAACTGCCC TCATACTTGC TGTATGTTGT GGATCAGCAA 1021 GTATAGTCAA TCTTCTACTT GAGCAAAATG TTGATGTATC TTCTCAAGAT CTATCTGGAC 1081 AGACGGCCAG AGAGTATGCT GTTTCTAGTC ATCATCATGT AATTTGTGAA TTACTTTCTG 1141 ACTATAAAGA AAAACAGATG CTAAAAATCT CTTCTGAAAA CAGCAATCCA GGTAAAGAAG 1201 CAGAAGAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCACA AGTATCCACC TTTAGCGGAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt