Construct: ORF TRCN0000481047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007143.2_s317c1
- Derived from:
- ccsbBroadEn_13639
- DNA Barcode:
- GACCCCCTCAGCAAATCTAACTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM74 (378108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516189.2 | 99.5% | 98.7% | 37T>C;382G>A;477A>C |
| 2 | human | 375593 | TRIM73 | tripartite motif containing 73 | NM_198924.3 | 99.2% | 98.8% | (many diffs) |
| 3 | human | 378108 | TRIM74 | tripartite motif containing 74 | NM_001317815.1 | 99.2% | 98.4% | (many diffs) |
| 4 | human | 378108 | TRIM74 | tripartite motif containing 74 | NM_198853.3 | 99.2% | 98.4% | (many diffs) |
| 5 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516187.3 | 99.2% | 98.4% | (many diffs) |
| 6 | human | 135892 | TRIM50 | tripartite motif containing 50 | XM_011515787.1 | 78.7% | 76.3% | (many diffs) |
| 7 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516190.2 | 68.5% | 68% | (many diffs) |
| 8 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_001281450.1 | 49.2% | 47.7% | (many diffs) |
| 9 | human | 135892 | TRIM50 | tripartite motif containing 50 | XM_011515789.2 | 49.2% | 47.7% | (many diffs) |
| 10 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_001281451.1 | 49.1% | 47.6% | (many diffs) |
| 11 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_178125.3 | 49.1% | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 813
- ORF length:
- 747
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttggcaggtg agcctgctgg agctggagga ccggcttcag tgtcccatct 121 gcctggaggt cttcaaggag tccctaatgc tacagtgcgg ccactcctac tgcaagggct 181 gcctggtttc cctgtcctac cacctggaca ccaaggtgcg ctgccccatg tgctggcagg 241 tggtggacgg cagcagctcc ttgcccaacg tctccctggc ctgggtgatc gaagccctga 301 ggctccctgg ggacccggag cccaaggtct gcgtgcacca ccggaacccg ctcagccttt 361 tctgcgagaa ggaccaggag ctcatctgtg gcctcTGCGG TCTGCTGGGC TCCCACCAAC 421 ACCACCCGGT CACGCCCGTC TCCACCATCT GCAGCCGCAT GAAGGAGGAG CTCGCAGCCC 481 TCTTCTCTGA GCTGAAGCAG GAGCAGAAGA AGGTGGATGA GCTCATCGCC AAACTGGTGA 541 ACAACCGGAC CCGAATCGTC AATGAGTCGG ATGTCTTCAG CTGGGTGATC CGCCGCGAGT 601 TCCAGGAGCT GCGCCACCCG GTGGACGAGG AGAAGGCCCG CTGCCTGGAG GGGATAGGGG 661 GTCACACCCG TGGCCTGGTG GCCTCCCTGG ACATGCAGCT GGAGCAGGCC CAGGGAACCC 721 GGGAGCGGCT GGCCCAAGCC GAGTGTGTGC TGGAACAGTT CGGAAATGAG GACCACCATG 781 AGTTCATCTG GTTCCACTCC ATGGCCTCCA GGTACCCAAC TTTCTTGTAC AAAGTGGTTG 841 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 901 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 961 CCCCCTCAGC AAATCTAACT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1021 ttgtgaaaga tt