Construct: ORF TRCN0000481067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018151.1_s317c1
- Derived from:
- ccsbBroadEn_09234
- DNA Barcode:
- ACGTCCCCAGTAGGGAACCTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIFM2 (84883)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84883 | AIFM2 | apoptosis inducing factor m... | NM_001198696.1 | 99.9% | 100% | 765G>A |
| 2 | human | 84883 | AIFM2 | apoptosis inducing factor m... | NM_032797.6 | 99.9% | 100% | 765G>A |
| 3 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | NM_001039194.3 | 86.8% | 90.3% | (many diffs) |
| 4 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | NM_001284300.1 | 86.8% | 90.3% | (many diffs) |
| 5 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | NM_178058.4 | 86.8% | 90.3% | (many diffs) |
| 6 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514124.3 | 86.8% | 90.3% | (many diffs) |
| 7 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514121.2 | 86.5% | 90.1% | (many diffs) |
| 8 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514122.3 | 86.5% | 90.1% | (many diffs) |
| 9 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_011243572.1 | 86.5% | 90.1% | (many diffs) |
| 10 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514125.1 | 65.6% | 68.1% | (many diffs) |
| 11 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514126.2 | 65.6% | 68.1% | (many diffs) |
| 12 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XM_006514127.3 | 65.6% | 68.1% | (many diffs) |
| 13 | mouse | 71361 | Aifm2 | apoptosis-inducing factor, ... | XR_380441.2 | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtcccaggtc tcggtggaat cgggagctct gcacgtggtg attgtgggtg 121 ggggctttgg cgggatcgca gcagccagcc agctgcaggc cctgaacgtc cccttcatgc 181 tggtggacat gaaggactcc ttccaccaca atgtggctgc tctccgagcc tccgtggaga 241 cagggttcgc caaaaagaca ttcatttctt actcggtgac tttcaaggac aacttccggc 301 aggggctagt agtggggata gacctgaaga accagatggt gctgctgcag ggtggcgagg 361 ccctgccctt ctctcatctt atcctggcca cgggcagcac tgggcccttc ccgggcaagt 421 ttaatgaggt ttccagccag caggccgcta tccaggccta tgaggacatg gtgaggcagg 481 tccagcgctc acggttcatc gtggtggtgg gaggaggctc ggctggagtg gagatggcag 541 cagagattaa aacagaatat cctgagaaag aggtcactct cattcactcc caagtggccc 601 tggctgacaa ggagctcctg ccctccgtcc ggcaggaagt gaaggagatc ctcctccGGA 661 AGGGCGTGCA GCTGCTGCTG AGTGAGCGGG TGAGCAATCT GGAGGAGCTG CCTCTCAATG 721 AGTATCGAGA GTACATCAAA GTGCAGACGG ACAAAGGCAC AGAGGTGGCC ACCAACCTGG 781 TGATTCTCTG CACCGGCATC AAGATCAACA GCTCCGCCTA CCGCAAAGCA TTTGAGAGCA 841 GACTAGCCAG CAGTGGTGCT CTGAGAGTGA ACGAGCACCT CCAGGTGGAG GGCCACAGCA 901 ACGTCTACGC CATTGGTGAC TGTGCCGACG TGAGGACGCC CAAGATGGCC TATCTTGCCG 961 GCCTCCACGC CAACATCGCC GTGGCCAACA TCGTCAACTC TGTGAAGCAG CGGCCTCTCC 1021 AGGCCTACAA GCCGGGTGCA CTGACGTTCC TCCTGTCCAT GGGGAGAAAT GACGGTGTGG 1081 GCCAAATCAG TGGCTTCTAT GTGGGCCGGC TCATGGTTCG GCTGACCAAG AGCCGGGACC 1141 TGTTCGTCTC TACGAGCTGG AAAACCATGA GGCAGTCTCC ACCTTGCCCA ACTATCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA ACGTCCCCAG TAGGGAACCT ACCTACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt