Construct: ORF TRCN0000481078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011910.2_s317c1
- Derived from:
- ccsbBroadEn_02517
- DNA Barcode:
- GTATTACCAGGTGCGATTAGCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFPL2 (10739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001159545.2 | 100% | 100% | |
| 2 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001159546.2 | 100% | 100% | |
| 3 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001364984.1 | 100% | 100% | |
| 4 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001364985.1 | 100% | 100% | |
| 5 | human | 10738 | RFPL3 | ret finger protein like 3 | NM_006604.2 | 97.4% | 94.4% | (many diffs) |
| 6 | human | 5988 | RFPL1 | ret finger protein like 1 | XM_011530298.3 | 95.4% | 90.6% | (many diffs) |
| 7 | human | 5988 | RFPL1 | ret finger protein like 1 | XM_011530299.3 | 95.4% | 90.6% | (many diffs) |
| 8 | human | 5988 | RFPL1 | ret finger protein like 1 | XM_017028900.2 | 95.4% | 90.6% | (many diffs) |
| 9 | human | 5988 | RFPL1 | ret finger protein like 1 | XM_017028902.2 | 95.4% | 90.6% | (many diffs) |
| 10 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001364982.1 | 92.9% | 92.9% | 1_66del |
| 11 | human | 10738 | RFPL3 | ret finger protein like 3 | NM_001098535.1 | 88.5% | 85.8% | (many diffs) |
| 12 | human | 5988 | RFPL1 | ret finger protein like 1 | NM_021026.2 | 86.7% | 82.3% | (many diffs) |
| 13 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001098527.3 | 76.1% | 76.1% | 1_270del |
| 14 | human | 10739 | RFPL2 | ret finger protein like 2 | XM_011529832.2 | 76.1% | 76.1% | 1_270del |
| 15 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001364983.1 | 69.2% | 69.2% | 1_384del |
| 16 | human | 10739 | RFPL2 | ret finger protein like 2 | NM_001364986.1 | 69.2% | 69.2% | 1_384del |
| 17 | human | 10739 | RFPL2 | ret finger protein like 2 | XM_011529826.2 | 69.2% | 69.2% | 1_384del |
| 18 | human | 10739 | RFPL2 | ret finger protein like 2 | XM_011529827.3 | 69.2% | 69.2% | 1_384del |
| 19 | human | 10739 | RFPL2 | ret finger protein like 2 | XM_011529828.2 | 69.2% | 69.2% | 1_384del |
| 20 | human | 10738 | RFPL3 | ret finger protein like 3 | XM_017028534.1 | 41.3% | 33.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcactcttc caagaagcaa gcagctgtcc cgtctgctca gactatctgg 121 aaaaaccaat gtccctggag tgtggatgcg ccgtctgcct caagtgcatt aattcactgc 181 agaaggagcc ccatggggag gatctacttt gctgttgctc ttccatggtc tctcggaaga 241 acaaaatcag gcgcaatcgg cagctagaga ggctggcttc ccacatcaag gaactggagc 301 ccaagctgaa gaagattctg cagatgaacc caaggatgcg gaagttccaa gtggatatga 361 ccttggatgc caacacagcc aacaacttcc tcctcatttc tgacgacctc aggagcgtcc 421 gaagtgggcg catcagacag aatcggcaag accttgccga gagatttgac gtgtccgttt 481 gcaTCCTGGG CTCCCCTCGC TTTACCTGTG GCCGCCACTG CTGGGAGGTG GACGTGGGAA 541 CAAGCACAGA ATGGGACCTG GGAGTCTGCA GAGAATCTGT TCACCGCAAA GGGAGGATCC 601 AGCTGACCAC AGAGCTTGGA TTCTGGACTG TGAGTTTGAG GGATGGAGGC CGCCTCTCTG 661 CCACCACGGT GCCGCTGACT TTCCTCTTCG TAGACCGCAA GTTACAGCGA GTGGGGATTT 721 TTCTGGATAT GGGCATGCAG AACGTTTCCT TTTTTGATGC TGAAAGTGGT TCCCATGTCT 781 ATACATTCAG GAGCGTATCT GCTGAGGAGC CATTGCGCCC ATTTTTGGCT CCTTCAGTTC 841 CACCTAATGG TGATCAAGGT GTCTTGAGCA TCTGTCCTTT GATGAACTCA GGCACTACTG 901 ATGCTCCAGT CCGTCCTGGG GAGGCCAAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTATT 1081 ACCAGGTGCG ATTAGCTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt