Construct: ORF TRCN0000481079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011268.1_s317c1
- Derived from:
- ccsbBroadEn_15929
- DNA Barcode:
- GAACTCTTTGTCAGTCCGACACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUS3L (56931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | NM_001161619.2 | 100% | 100% | |
| 2 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | XM_017027020.1 | 64% | 63% | (many diffs) |
| 3 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | NM_020175.3 | 62.7% | 62.7% | 174_899del |
| 4 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | XR_244033.1 | 56.6% | (many diffs) | |
| 5 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | XR_936193.2 | 54% | (many diffs) | |
| 6 | human | 56931 | DUS3L | dihydrouridine synthase 3 like | XR_002958335.1 | 36.5% | (many diffs) | |
| 7 | mouse | 224907 | Dus3l | dihydrouridine synthase 3-l... | NM_144858.2 | 53.1% | 56% | (many diffs) |
| 8 | mouse | 224907 | Dus3l | dihydrouridine synthase 3-l... | XM_006524161.3 | 48.6% | 51.2% | (many diffs) |
| 9 | mouse | 224907 | Dus3l | dihydrouridine synthase 3-l... | XM_006524162.3 | 48.1% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagggaacg gcggaggctc ctctagagaa tggtggtggt ggcgactcgg 121 gagccggagc tttggaacga ggagtggcgc ccattaagcg tcaatacctc accaccaagg 181 agcagtttca ccaattcctg gaagccaaag ggcaggagaa gacttgccgg gaaaccgagc 241 tggacatccg tggcaaactt tacctggccc ccctcaccac gtgtgggaac ctgcccttcc 301 gacggatctg caagcgcttc ggggcggatg tgacatgtgg agagatggcc gtctgcacca 361 acctgctgca gggccagatg tccgagtggg ccctactcaa acgccaccag tgtgaggaca 421 tctttggcgt ccagctggag ggcgccttcc ccgacaccat gaccaagtgt gccgagctgc 481 tgagccgcac cgtggaggtg gactttgtgg acatcaacgt cggctgcccc atcgacctcg 541 tgtacaagaa gggtgggggc tgtgccctca tgaatcgctc caccaagttc cagcagatcg 601 tccgtggcat gaaccaggtg ctggatgtgc cgctgactgt gaagatccgc acaggcgtcc 661 aggagcgtgt gaacctggcg caccgcctgc tgcccgagct gcgggactgg ggcgtggcac 721 tcgtcacgct ccacggccgc tctcgggagc agcgctacac caagctagcc gactggcagt 781 acatcgagga gtgcgtgcag gccgccagcc ccatgcccct gttcggaaat ggggacatct 841 tgtcattTGA GGATGCCAAC CGCGCCATGC AGACTGGTGT CACCGGGATC ATGATTGCCC 901 GTGGCGCCCT GCTCAAGCCG TGGCTCTTCA CGGAGATCAA GGAGCAGCGG CACTGGGACA 961 TCTCGTCGTC CGAGCGCCTG GACATCCTGC GGGACTTCAC CAACTACGGC CTGGAGCACT 1021 GGGGCTCGGA CACGCAGGGC GTGGAGAAGA CCCGGCGCTT TCTGCTCGAG TGGCTGTCCT 1081 TCCTGTGCCG GTACGTGCCC GTGGGGCTGC TGGAGCGGCT CCCACAGAGG ATCAACGAGC 1141 GGCCGCCCTA CTACCTGGGC CGCGACTACC TGGAGACGCT GATGGCCAGC CAGAAGGCAG 1201 CCGACTGGAT CCGCATCAGC GAGATGCTCC TTGGGCCAGT GCCCCCCAGC TTCGCCTTCT 1261 TGCCGAAGCA CAAGGCCAAC GCGTACAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAACT 1441 CTTTGTCAGT CCGACACAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt