Construct: ORF TRCN0000481079

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011268.1_s317c1
Derived from:
ccsbBroadEn_15929
DNA Barcode:
GAACTCTTTGTCAGTCCGACACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DUS3L (56931)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481079
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56931 DUS3L dihydrouridine synthase 3 like NM_001161619.2 100% 100%
2 human 56931 DUS3L dihydrouridine synthase 3 like XM_017027020.1 64% 63% (many diffs)
3 human 56931 DUS3L dihydrouridine synthase 3 like NM_020175.3 62.7% 62.7% 174_899del
4 human 56931 DUS3L dihydrouridine synthase 3 like XR_244033.1 56.6% (many diffs)
5 human 56931 DUS3L dihydrouridine synthase 3 like XR_936193.2 54% (many diffs)
6 human 56931 DUS3L dihydrouridine synthase 3 like XR_002958335.1 36.5% (many diffs)
7 mouse 224907 Dus3l dihydrouridine synthase 3-l... NM_144858.2 53.1% 56% (many diffs)
8 mouse 224907 Dus3l dihydrouridine synthase 3-l... XM_006524161.3 48.6% 51.2% (many diffs)
9 mouse 224907 Dus3l dihydrouridine synthase 3-l... XM_006524162.3 48.1% 50.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1290
ORF length:
1224
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggagggaacg gcggaggctc ctctagagaa tggtggtggt ggcgactcgg
121gagccggagc tttggaacga ggagtggcgc ccattaagcg tcaatacctc accaccaagg
181agcagtttca ccaattcctg gaagccaaag ggcaggagaa gacttgccgg gaaaccgagc
241tggacatccg tggcaaactt tacctggccc ccctcaccac gtgtgggaac ctgcccttcc
301gacggatctg caagcgcttc ggggcggatg tgacatgtgg agagatggcc gtctgcacca
361acctgctgca gggccagatg tccgagtggg ccctactcaa acgccaccag tgtgaggaca
421tctttggcgt ccagctggag ggcgccttcc ccgacaccat gaccaagtgt gccgagctgc
481tgagccgcac cgtggaggtg gactttgtgg acatcaacgt cggctgcccc atcgacctcg
541tgtacaagaa gggtgggggc tgtgccctca tgaatcgctc caccaagttc cagcagatcg
601tccgtggcat gaaccaggtg ctggatgtgc cgctgactgt gaagatccgc acaggcgtcc
661aggagcgtgt gaacctggcg caccgcctgc tgcccgagct gcgggactgg ggcgtggcac
721tcgtcacgct ccacggccgc tctcgggagc agcgctacac caagctagcc gactggcagt
781acatcgagga gtgcgtgcag gccgccagcc ccatgcccct gttcggaaat ggggacatct
841tgtcattTGA GGATGCCAAC CGCGCCATGC AGACTGGTGT CACCGGGATC ATGATTGCCC
901GTGGCGCCCT GCTCAAGCCG TGGCTCTTCA CGGAGATCAA GGAGCAGCGG CACTGGGACA
961TCTCGTCGTC CGAGCGCCTG GACATCCTGC GGGACTTCAC CAACTACGGC CTGGAGCACT
1021GGGGCTCGGA CACGCAGGGC GTGGAGAAGA CCCGGCGCTT TCTGCTCGAG TGGCTGTCCT
1081TCCTGTGCCG GTACGTGCCC GTGGGGCTGC TGGAGCGGCT CCCACAGAGG ATCAACGAGC
1141GGCCGCCCTA CTACCTGGGC CGCGACTACC TGGAGACGCT GATGGCCAGC CAGAAGGCAG
1201CCGACTGGAT CCGCATCAGC GAGATGCTCC TTGGGCCAGT GCCCCCCAGC TTCGCCTTCT
1261TGCCGAAGCA CAAGGCCAAC GCGTACAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1321TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1381CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAACT
1441CTTTGTCAGT CCGACACAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1501tgaaagatt

Download FASTA (ORF) (Full)