Transcript: Mouse XM_006524161.3

PREDICTED: Mus musculus dihydrouridine synthase 3-like (S. cerevisiae) (Dus3l), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dus3l (224907)
Length:
2254
CDS:
129..2219

Additional Resources:

NCBI RefSeq record:
XM_006524161.3
NBCI Gene record:
Dus3l (224907)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006524161.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000375503 AGAGGCCACCCTATTACTTAG pLKO_005 2065 CDS 100% 10.800 15.120 N Dus3l n/a
2 TRCN0000173669 CCACGAATATCTGGATGGAGA pLKO.1 239 CDS 100% 2.640 3.696 N Dus3l n/a
3 TRCN0000366622 GACTTCACTCACTACGGTTTA pLKO_005 1920 CDS 100% 10.800 7.560 N Dus3l n/a
4 TRCN0000366693 GGCAATTGAATCCCGTGAAAG pLKO_005 365 CDS 100% 10.800 7.560 N Dus3l n/a
5 TRCN0000173147 CATGTGCATTTGGAGACCGTT pLKO.1 496 CDS 100% 2.640 1.848 N Dus3l n/a
6 TRCN0000173248 CCAAAGCAGAATAGCTGCCAT pLKO.1 855 CDS 100% 2.640 1.848 N Dus3l n/a
7 TRCN0000173707 CGGTTCCACGAATATCTGGAT pLKO.1 234 CDS 100% 2.640 1.848 N Dus3l n/a
8 TRCN0000173326 GAAAGCTGTATCTGGCTCCTT pLKO.1 1003 CDS 100% 2.640 1.848 N Dus3l n/a
9 TRCN0000375479 CCGCCAAGTTCCAACAGATTG pLKO_005 1330 CDS 100% 10.800 6.480 N Dus3l n/a
10 TRCN0000366692 GGCTGTTCACGGAGATCAAAG pLKO_005 1849 CDS 100% 10.800 6.480 N Dus3l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006524161.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15929 pDONR223 0% 48.6% 51.2% None (many diffs) n/a
2 ccsbBroad304_15929 pLX_304 0% 48.6% 51.2% V5 (many diffs) n/a
3 TRCN0000481079 GAACTCTTTGTCAGTCCGACACAG pLX_317 36.4% 48.6% 51.2% V5 (many diffs) n/a
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