Construct: ORF TRCN0000481101

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017038.1_s317c1
Derived from:
ccsbBroadEn_12539
DNA Barcode:
AGGGAACGCGCGGTGGCAGAGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG9A (79065)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481101
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79065 ATG9A autophagy related 9A NM_001077198.3 62% 60.1% 1_288del;1781delC;1853_2517del
2 human 79065 ATG9A autophagy related 9A NM_024085.5 62% 60.1% 1_288del;1781delC;1853_2517del
3 human 79065 ATG9A autophagy related 9A NR_104255.2 39.8% (many diffs)
4 mouse 245860 Atg9a autophagy related 9A XM_011238690.2 60.4% 63.2% (many diffs)
5 mouse 245860 Atg9a autophagy related 9A XM_011238689.2 57.8% 60.5% (many diffs)
6 mouse 245860 Atg9a autophagy related 9A NM_001003917.4 57.1% 59.8% (many diffs)
7 mouse 245860 Atg9a autophagy related 9A NM_001288612.1 57.1% 59.8% (many diffs)
8 mouse 245860 Atg9a autophagy related 9A XM_011238687.2 57.1% 59.8% (many diffs)
9 mouse 245860 Atg9a autophagy related 9A XM_011238688.2 57.1% 59.8% (many diffs)
10 mouse 245860 Atg9a autophagy related 9A NM_001288613.1 49.3% 51.1% (many diffs)
11 mouse 245860 Atg9a autophagy related 9A NR_109938.1 35.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1629
ORF length:
1563
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gaaccacagt cttcacccta ctgaacccgt caaggtcact ctgccagacg
121cctttttgcc tgctcaagtc tgtagtgcca ggattcagga aaatggctcc cttatcacca
181tcctggtcat tgctggtgtc ttctggatcc accggcttat caagttcatc tataacattt
241gctgctactg ggagatccac tccttctacc tgcacgctct gcgcatccct atgtctgccc
301ttccgtattg cacgtggcaa gaagtgcagg cccggatcgt gcagacgcag aaggagcacc
361agatctgcat ccacaaacgt gagctgacag aactggacat ctaccaccgc atcctccgtt
421tccagaacta catggtggca ctggttaaca aatccctcct gcctctgcgc ttccgcctgc
481ctggcctcgg ggaagctgtc ttcttcaccc gtggtctcaa gtacaacttt gagctgatcc
541tcttctgggg acctggctct ctgtttctca atgaatggag cctcaaggcc gagtacaaac
601gtggggggca acggctagag ctggcccagc gcctcagcaa ccgcatcctg tggattggca
661tcgctaactt cctgctgtgc cccctcatcc tcatatggca aatcctctat gccttcttca
721gctatgctga ggtgctgaag cgggagccgg gggccctggg agcacgctgc tggtcactct
781atggccgctg ctacctccgc cacttcaacg agctggagca cgagctgcag tcccgcctca
841accgtggcta caagcccgcc tccaagtaca tgaattgctt cttgtcacct cttttgacac
901tgctggccaa gaatggagcc ttcttcgctg gctccatcct ggctgtgctt attgccctca
961ccatttatga cgaagatgtg ttggctgtgg aacatgtgct gaccaccgtc acactcctgg
1021gggtcaccgt gaccgtgtgc aggtccttta tcccggacca gcacatggtg ttctgccctg
1081agcagctgct ccgcgtgatc ctcgctcaca tccactacat gcctgaccac tggcagggta
1141atgcccaccg ctcgcagacc cgggacgagt ttgcccagct cttccagtac aaggcagtgt
1201tcattttgga agagttgctg agccccattg tcacacccct caTCCTCATC TTCTGCCTGC
1261GCCCACGGGC CCTGGAGATT ATAGACTTCT TCCGAAACTT CACCGTGGAG GTCGTTGGTG
1321TGGGAGATAC CTGCTCCTTT GCTCAGATGG ATGTTCGCCA GCATGGTCAT CCCCAGTGGC
1381TATCTGCTGG GCAGACAGAG GCCTCAGTGT ACCAGCAAGC TGAGGATGGA AAGACAGAGT
1441TGTCACTCAT GCACTTTGCC ATCACCAACC CTGGCTGGCA GCCACCACGT GAGAGCACAG
1501CCTTCCTAGG CTTCCTCAAG GAGCAGGTTC AGCGGGATGG AGCAGCTGCT AGCCTCGCCA
1561AGGGGGTCTG CTCCCTGAAA ATGCCCTCTT TACGTCTATC CAGTCCTTAC AATCTGAGTC
1621TGAGCCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1681CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1741TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGGAA CGCGCGGTGG CAGAGAACAC
1801GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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