Construct: ORF TRCN0000481101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017038.1_s317c1
- Derived from:
- ccsbBroadEn_12539
- DNA Barcode:
- AGGGAACGCGCGGTGGCAGAGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG9A (79065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79065 | ATG9A | autophagy related 9A | NM_001077198.3 | 62% | 60.1% | 1_288del;1781delC;1853_2517del |
| 2 | human | 79065 | ATG9A | autophagy related 9A | NM_024085.5 | 62% | 60.1% | 1_288del;1781delC;1853_2517del |
| 3 | human | 79065 | ATG9A | autophagy related 9A | NR_104255.2 | 39.8% | (many diffs) | |
| 4 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238690.2 | 60.4% | 63.2% | (many diffs) |
| 5 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238689.2 | 57.8% | 60.5% | (many diffs) |
| 6 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001003917.4 | 57.1% | 59.8% | (many diffs) |
| 7 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001288612.1 | 57.1% | 59.8% | (many diffs) |
| 8 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238687.2 | 57.1% | 59.8% | (many diffs) |
| 9 | mouse | 245860 | Atg9a | autophagy related 9A | XM_011238688.2 | 57.1% | 59.8% | (many diffs) |
| 10 | mouse | 245860 | Atg9a | autophagy related 9A | NM_001288613.1 | 49.3% | 51.1% | (many diffs) |
| 11 | mouse | 245860 | Atg9a | autophagy related 9A | NR_109938.1 | 35.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaaccacagt cttcacccta ctgaacccgt caaggtcact ctgccagacg 121 cctttttgcc tgctcaagtc tgtagtgcca ggattcagga aaatggctcc cttatcacca 181 tcctggtcat tgctggtgtc ttctggatcc accggcttat caagttcatc tataacattt 241 gctgctactg ggagatccac tccttctacc tgcacgctct gcgcatccct atgtctgccc 301 ttccgtattg cacgtggcaa gaagtgcagg cccggatcgt gcagacgcag aaggagcacc 361 agatctgcat ccacaaacgt gagctgacag aactggacat ctaccaccgc atcctccgtt 421 tccagaacta catggtggca ctggttaaca aatccctcct gcctctgcgc ttccgcctgc 481 ctggcctcgg ggaagctgtc ttcttcaccc gtggtctcaa gtacaacttt gagctgatcc 541 tcttctgggg acctggctct ctgtttctca atgaatggag cctcaaggcc gagtacaaac 601 gtggggggca acggctagag ctggcccagc gcctcagcaa ccgcatcctg tggattggca 661 tcgctaactt cctgctgtgc cccctcatcc tcatatggca aatcctctat gccttcttca 721 gctatgctga ggtgctgaag cgggagccgg gggccctggg agcacgctgc tggtcactct 781 atggccgctg ctacctccgc cacttcaacg agctggagca cgagctgcag tcccgcctca 841 accgtggcta caagcccgcc tccaagtaca tgaattgctt cttgtcacct cttttgacac 901 tgctggccaa gaatggagcc ttcttcgctg gctccatcct ggctgtgctt attgccctca 961 ccatttatga cgaagatgtg ttggctgtgg aacatgtgct gaccaccgtc acactcctgg 1021 gggtcaccgt gaccgtgtgc aggtccttta tcccggacca gcacatggtg ttctgccctg 1081 agcagctgct ccgcgtgatc ctcgctcaca tccactacat gcctgaccac tggcagggta 1141 atgcccaccg ctcgcagacc cgggacgagt ttgcccagct cttccagtac aaggcagtgt 1201 tcattttgga agagttgctg agccccattg tcacacccct caTCCTCATC TTCTGCCTGC 1261 GCCCACGGGC CCTGGAGATT ATAGACTTCT TCCGAAACTT CACCGTGGAG GTCGTTGGTG 1321 TGGGAGATAC CTGCTCCTTT GCTCAGATGG ATGTTCGCCA GCATGGTCAT CCCCAGTGGC 1381 TATCTGCTGG GCAGACAGAG GCCTCAGTGT ACCAGCAAGC TGAGGATGGA AAGACAGAGT 1441 TGTCACTCAT GCACTTTGCC ATCACCAACC CTGGCTGGCA GCCACCACGT GAGAGCACAG 1501 CCTTCCTAGG CTTCCTCAAG GAGCAGGTTC AGCGGGATGG AGCAGCTGCT AGCCTCGCCA 1561 AGGGGGTCTG CTCCCTGAAA ATGCCCTCTT TACGTCTATC CAGTCCTTAC AATCTGAGTC 1621 TGAGCCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1681 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1741 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGGAA CGCGCGGTGG CAGAGAACAC 1801 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt