Construct: ORF TRCN0000481111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010017.2_s317c1
- Derived from:
- ccsbBroadEn_01333
- DNA Barcode:
- GTTAACATTTTCCCTTTAATCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481111
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5737 | PTGFR | prostaglandin F receptor | NM_000959.4 | 100% | 100% | |
| 2 | human | 5737 | PTGFR | prostaglandin F receptor | NM_001039585.2 | 81.5% | 75.7% | (many diffs) |
| 3 | human | 5737 | PTGFR | prostaglandin F receptor | XM_017001873.1 | 80.1% | 75.4% | (many diffs) |
| 4 | human | 5737 | PTGFR | prostaglandin F receptor | XR_001737338.1 | 57.1% | 1_335del;1134_1274del;1363_1364ins190 | |
| 5 | human | 5737 | PTGFR | prostaglandin F receptor | XR_946715.2 | 53.5% | (many diffs) | |
| 6 | mouse | 19220 | Ptgfr | prostaglandin F receptor | NM_008966.3 | 84% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catgaacaat tccaaacagc tagtgtctcc tgcagctgcg cttctttcaa 121 acacaacctg ccagacggaa aaccggcttt ccgtattttt ttcagtaatc ttcatgacag 181 tgggaatctt gtcaaacagc cttgccatcg ccattctcat gaaggcatat cagagattta 241 gacagaagtc caaggcatcg tttctgcttt tggccagtgg cctggtaatc actgatttct 301 ttggccatct catcaatgga gccatagcag tatttgtata tgcttctgat aaagaatgga 361 tccgctttga ccaatcaaat gtcctttgca gtatttttgg tatctgcatg gtgttttctg 421 gtctgtgccc acttcttcta ggcagtgtga tggccattga gcggtgtatt ggagtcacaa 481 aaccaatatt tcattctacg aaaattacat ccaaacatgt gaaaatgatg ttaagtggtg 541 tgtgcttgtt tgctgttttc atagctttgc tgcccatcct tggacatcga gactataaaa 601 ttcaggcgtc gaggacctgg tgtttctaca acacagaaga catcaaagac tgggaagata 661 gattttatct tcTACTTTTT TCTTTTCTGG GGCTCTTAGC CCTTGGTGTT TCATTGTTGT 721 GCAATGCAAT CACAGGAATT ACACTTTTAA GAGTTAAATT TAAAAGTCAG CAGCACAGAC 781 AAGGCAGATC TCATCATTTG GAAATGGTAA TCCAGCTCCT GGCGATAATG TGTGTCTCCT 841 GTATTTGTTG GAGCCCATTT CTGGTTACAA TGGCCAACAT TGGAATAAAT GGAAATCATT 901 CTCTGGAAAC CTGTGAAACA ACACTTTTTG CTCTCCGAAT GGCAACATGG AATCAAATCT 961 TAGATCCTTG GGTATATATT CTTCTACGAA AGGCTGTCCT TAAGAATCTC TATAAGCTTG 1021 CCAGTCAATG CTGTGGAGTG CATGTCATCA GCTTACATAT TTGGGAGCTT AGTTCCATTA 1081 AAAATTCCTT AAAGGTTGCT GCTATTTCTG AGTCACCAGT TGCAGAGAAA TCAGCAAGCA 1141 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAGT TAACATTTTC CCTTTAATCA TCACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt