Transcript: Mouse NM_008966.3

Mus musculus prostaglandin F receptor (Ptgfr), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Ptgfr (19220)
Length:
4414
CDS:
300..1400

Additional Resources:

NCBI RefSeq record:
NM_008966.3
NBCI Gene record:
Ptgfr (19220)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008966.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329169 AGGAGTCACCAATCCTATATT pLKO_005 704 CDS 100% 15.000 21.000 N Ptgfr n/a
2 TRCN0000329168 TCCTTGGACACCGAGATTATC pLKO_005 811 CDS 100% 13.200 18.480 N Ptgfr n/a
3 TRCN0000329171 TCTACGAAGATCACATCTAAA pLKO_005 729 CDS 100% 13.200 18.480 N Ptgfr n/a
4 TRCN0000329172 TGATCCGACTCATCGTGTAAC pLKO_005 1451 3UTR 100% 10.800 15.120 N Ptgfr n/a
5 TRCN0000027865 CCAATCCTATATTTCACTCTA pLKO.1 712 CDS 100% 4.950 6.930 N Ptgfr n/a
6 TRCN0000027927 CACCTTATCAACGGAGGCATA pLKO.1 540 CDS 100% 4.050 3.240 N Ptgfr n/a
7 TRCN0000027916 CCCTGGGTCTATATTCTGCTA pLKO.1 1200 CDS 100% 2.640 2.112 N Ptgfr n/a
8 TRCN0000329170 TTAGATCCCTGGGTCTATATT pLKO_005 1194 CDS 100% 15.000 10.500 N Ptgfr n/a
9 TRCN0000027910 CCTTAAAGGTTGCTGCTATTT pLKO.1 1321 CDS 100% 13.200 9.240 N Ptgfr n/a
10 TRCN0000027866 GCTGTCCTTAGGAACCTGTAT pLKO.1 1227 CDS 100% 4.950 3.465 N Ptgfr n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008966.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489748 CTCCGACCCGTGTTGTGCATATAA pLX_317 40.5% 84.1% 87.4% V5 (many diffs) n/a
2 ccsbBroadEn_01333 pDONR223 100% 84% 86.8% None (many diffs) n/a
3 ccsbBroad304_01333 pLX_304 0% 84% 86.8% V5 (many diffs) n/a
4 TRCN0000481111 GTTAACATTTTCCCTTTAATCATC pLX_317 43.8% 84% 86.8% V5 (many diffs) n/a
5 TRCN0000489925 CATCATACTGCGAACTCACATAAT pLX_317 40.7% 84% 86.8% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV