Transcript: Human NM_000959.4

Homo sapiens prostaglandin F receptor (PTGFR), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
PTGFR (5737)
Length:
5429
CDS:
336..1415

Additional Resources:

NCBI RefSeq record:
NM_000959.4
NBCI Gene record:
PTGFR (5737)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000959.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358055 CTTGGACATCGAGACTATAAA pLKO_005 849 CDS 100% 15.000 21.000 N PTGFR n/a
2 TRCN0000014169 GCGGTGTATTGGAGTCACAAA pLKO.1 731 CDS 100% 4.950 6.930 N PTGFR n/a
3 TRCN0000014172 GCTGTCCTTAAGAATCTCTAT pLKO.1 1263 CDS 100% 4.950 6.930 N PTGFR n/a
4 TRCN0000357986 ATGGCCATTGAGCGGTGTATT pLKO_005 720 CDS 100% 13.200 10.560 N PTGFR n/a
5 TRCN0000358019 TCTTAGATCCTTGGGTATATA pLKO_005 1228 CDS 100% 15.000 10.500 N PTGFR n/a
6 TRCN0000027910 CCTTAAAGGTTGCTGCTATTT pLKO.1 1357 CDS 100% 13.200 9.240 N Ptgfr n/a
7 TRCN0000358067 GGCAATATTTCAGTTAGTTAA pLKO_005 1468 3UTR 100% 13.200 9.240 N PTGFR n/a
8 TRCN0000014170 CCTGGTAATCACTGATTTCTT pLKO.1 551 CDS 100% 5.625 3.938 N PTGFR n/a
9 TRCN0000014168 GCCTGCATAGTGAAATGGTTA pLKO.1 1761 3UTR 100% 4.950 3.465 N PTGFR n/a
10 TRCN0000014171 GCTTACATATTTGGGAGCTTA pLKO.1 1321 CDS 100% 4.950 3.465 N PTGFR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000959.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01333 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01333 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000481111 GTTAACATTTTCCCTTTAATCATC pLX_317 43.8% 100% 100% V5 n/a
4 TRCN0000489925 CATCATACTGCGAACTCACATAAT pLX_317 40.7% 100% 100% V5 (not translated due to prior stop codon) n/a
5 TRCN0000489748 CTCCGACCCGTGTTGTGCATATAA pLX_317 40.5% 99.9% 99.7% V5 1077_1078insG n/a
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