Construct: ORF TRCN0000481116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006141.1_s317c1
- Derived from:
- ccsbBroadEn_06446
- DNA Barcode:
- AATCCCCGCATTTGGATCTGTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL12RB2 (3595)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001374259.1 | 99.8% | 99.8% | 714T>C;1278G>C;2337C>A |
| 2 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001559.2 | 99.8% | 99.8% | 714T>C;1278G>C;2337C>A |
| 3 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_005270827.2 | 99.8% | 99.8% | 714T>C;1278G>C;2337C>A |
| 4 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_005270828.3 | 99.8% | 99.8% | 714T>C;1278G>C;2337C>A |
| 5 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_011541383.2 | 99.8% | 99.8% | 714T>C;1278G>C;2337C>A |
| 6 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_011541384.2 | 93.7% | 93.7% | (many diffs) |
| 7 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258215.1 | 89.9% | 89.9% | (many diffs) |
| 8 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_006710617.2 | 89.9% | 89.9% | (many diffs) |
| 9 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258214.1 | 76.3% | 75.1% | (many diffs) |
| 10 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001319233.1 | 76.3% | 75.1% | (many diffs) |
| 11 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_017001203.1 | 76.3% | 75.1% | (many diffs) |
| 12 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NM_001258216.1 | 73.5% | 71.9% | (many diffs) |
| 13 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NR_047584.1 | 62.4% | (many diffs) | |
| 14 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | NR_047583.1 | 57.7% | (many diffs) | |
| 15 | human | 3595 | IL12RB2 | interleukin 12 receptor sub... | XM_017001204.1 | 57.1% | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2655
- ORF length:
- 2586
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcacatact tttagaggat gctcattggc atttatgttt ataatcacgt 121 ggctgttgat taaagcaaaa atagatgcgt gcaagagagg cgatgtgact gtgaagcctt 181 cccatgtaat tttacttgga tccactgtca atattacatg ctctttgaag cccagacaag 241 gctgctttca ctattccaga cgtaacaagt taatcctgta caagtttgac agaagaatca 301 attttcacca tggccactcc ctcaattctc aagtcacagg tcttcccctt ggtacaacct 361 tgtttgtctg caaactggcc tgtatcaata gtgatgaaat tcaaatatgt ggagcagaga 421 tcttcgttgg tgttgctcca gaacagcctc aaaatttatc ctgcatacag aagggagaac 481 aggggactgt ggcctgcacc tgggaaagag gacgagacac ccacttatac actgagtata 541 ctctacagct aagtggacca aaaaatttaa cctggcagaa gcaatgtaaa gacatttatt 601 gtgactattt ggactttgga atcaacctca cccctgaatc acctgaatcc aatttcacag 661 ccaaggttac tgctgtcaat agtcttggaa gctcctcttc acttccatcc acattcacat 721 tcttggacat agtgaggcct cttcctccgt gggacattag aatcaaattt caaaaggctt 781 ccgtgagcag atgtaccctt tattggagag atgagggact ggtactgctt aatcgactca 841 gatatcggcc cagtaacagc aggctctgga atatggttaa tgttacaaag gccaaaggaa 901 gacatgattt gctggatctg aaaccattta cagaatatga atttcagatt tcctctaagc 961 tacatcttta taagggaagt tggagtgatt ggagtgaatc attgagagca caaacaccag 1021 aagaagagcc tactgggatg ttagatgtct ggtacatgaa acggcacatt gactacagta 1081 gacaacagat ttctcttttc tggaagaatc tgagtgtctc agaggcaaga ggaaaaattc 1141 tccactatca ggtgaccttg caggagctga caggagggaa agccatgaca cagaacatca 1201 caggacacac ctcctggacc acagtcattc ctagaaccgg aaattgggct gtggctgtgt 1261 ctgcagcaaa ttcaaaaggc agttctctgc ccactcgtat taacataatg aacctgtgtg 1321 aggcagggtt gctggctcct cgccacgtct ctgcaaactc agagggcatg gacaacattc 1381 tggtgacttg gcagcctccc aggaaagatc cctctgctgt tcaggagtac gtggtggaat 1441 ggagagagct ccatccaggg ggtgacacac aggtccctct aaactggcta cggagtcgac 1501 cctacaatgt gtctgctctg atttcagaga acataaaatc ctacatctgt tatgaaatcc 1561 gtgtgtatgc actctcaggg gatcaaggag gatgcagctc catcctgggt aactctaagc 1621 acaaagcacc actgagtggc ccccacatta atgccatcac agaggaaaag gggagcattt 1681 taatttcatg gaacagcatt ccagtccagg agcaaatggg ctgcctcctc cattatagga 1741 tatactggaa ggaacgggac tccaactccc agcctcagct ctgtgaaatt ccctacagag 1801 tctcccaaaa ttcacatcca ataaacagcc tgcagccccg agtgacatat gtcctgtgga 1861 tgacagctct gacagctgct ggtgaaagtt cccacggaaa tgagagggaa ttttgtctgc 1921 aaggtaaagc caattggatg gcgtttgtgg caccaagcat ttgcattgct atcatcatgg 1981 tgggcatttt ctcaacgcat tacttccagc aaaaggtgtt tgttctccta gcagccctca 2041 gacctcagtg gtgtagcaga gaaattccag atccagcaaa tagcacttgc gctaagaaat 2101 atcccattgc agaggagaag acacagctgc ccttggacag gctcctgata gactggccca 2161 cgcctgaaga tcctgaaccg ctggtcatca gtgaagtcct tcatcaagtg accccagttt 2221 tcagacatcc cccctgctcc aactggccac aaagggaaaa aggaatccaa ggtcatcagg 2281 ccTCTGAGAA AGACATGATG CACAGTGCCT CAAGCCCACC ACCTCCAAGA GCTCTCCAAG 2341 CTGAGAGCAG ACAACTGGTG GATCTGTACA AGGTGCTGGA GAGCAGGGGC TCCGACCCAA 2401 AGCCAGAAAA CCCAGCCTGT CCCTGGACGG TGCTCCCAGC AGGTGACCTT CCCACCCATG 2461 ATGGCTACTT ACCCTCCAAC ATAGATGACC TCCCCTCACA TGAGGCACCT CTCGCTGACT 2521 CTCTGGAAGA ACTGGAGCCT CAGCACATCT CCCTTTCTGT TTTCCCCTCA AGTTCTCTTC 2581 ACCCACTCAC CTTCTCCTGT GGTGATAAGC TGACTCTGGA TCAGTTAAAG ATGAGGTGTG 2641 ACTCCCTCAT GCTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2701 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2761 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATCCCCGCA TTTGGATCTG 2821 TTCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt