Transcript: Human XM_011541383.2

PREDICTED: Homo sapiens interleukin 12 receptor subunit beta 2 (IL12RB2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
IL12RB2 (3595)
Length:
4423
CDS:
194..2782

Additional Resources:

NCBI RefSeq record:
XM_011541383.2
NBCI Gene record:
IL12RB2 (3595)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011541383.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000058161 CCTCCTCCATTATAGGATATA pLKO.1 1849 CDS 100% 13.200 18.480 N IL12RB2 n/a
2 TRCN0000436750 TACGGAGTCGACCCTACAATG pLKO_005 1614 CDS 100% 10.800 15.120 N IL12RB2 n/a
3 TRCN0000413042 TTCCAGACGTAACAAGTTAAT pLKO_005 379 CDS 100% 13.200 9.240 N IL12RB2 n/a
4 TRCN0000427367 GCTACTTACCCTCCAACATAG pLKO_005 2589 CDS 100% 10.800 7.560 N IL12RB2 n/a
5 TRCN0000058158 CCTGTATCAATAGTGATGAAA pLKO.1 504 CDS 100% 5.625 3.938 N IL12RB2 n/a
6 TRCN0000058159 CCTGGGTAACTCTAAGCACAA pLKO.1 1729 CDS 100% 4.050 2.835 N IL12RB2 n/a
7 TRCN0000058160 CCCACTTATACACTGAGTATA pLKO.1 645 CDS 100% 1.320 0.924 N IL12RB2 n/a
8 TRCN0000067719 GCTCTGATTTCAGAGAACATA pLKO.1 1640 CDS 100% 0.563 0.394 N Il12rb2 n/a
9 TRCN0000058162 CCACGGAAATGAGAGGGAATT pLKO.1 2017 CDS 100% 0.000 0.000 N IL12RB2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3564 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011541383.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491277 ATCCTAACGTAATTTTTTAATGAC pLX_317 10.6% 99.9% 100% V5 (not translated due to prior stop codon) 714T>C n/a
2 ccsbBroadEn_06446 pDONR223 100% 99.8% 99.8% None 714T>C;1278G>C;2337C>A n/a
3 ccsbBroad304_06446 pLX_304 0% 99.8% 99.8% V5 714T>C;1278G>C;2337C>A n/a
4 TRCN0000481116 AATCCCCGCATTTGGATCTGTTCC pLX_317 14.6% 99.8% 99.8% V5 714T>C;1278G>C;2337C>A n/a
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