Construct: ORF TRCN0000481122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002608.1_s317c1
- Derived from:
- ccsbBroadEn_00896
- DNA Barcode:
- AACCCGACGGTCGGCATGCGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ12 (3768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481122
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3768 | KCNJ12 | potassium inwardly rectifyi... | NM_021012.5 | 100% | 100% | |
| 2 | human | 3768 | KCNJ12 | potassium inwardly rectifyi... | XM_005256625.5 | 100% | 100% | |
| 3 | human | 3768 | KCNJ12 | potassium inwardly rectifyi... | XM_011523831.2 | 100% | 100% | |
| 4 | human | 100134444 | KCNJ18 | potassium inwardly rectifyi... | NM_001194958.2 | 98.9% | 98.6% | (many diffs) |
| 5 | human | 100134444 | KCNJ18 | potassium inwardly rectifyi... | XM_005276919.3 | 80.1% | 79.8% | (many diffs) |
| 6 | mouse | 16515 | Kcnj12 | potassium inwardly-rectifyi... | NM_001267593.1 | 88.6% | 96% | (many diffs) |
| 7 | mouse | 16515 | Kcnj12 | potassium inwardly-rectifyi... | XM_006532318.3 | 88.6% | 96% | (many diffs) |
| 8 | mouse | 16515 | Kcnj12 | potassium inwardly-rectifyi... | NM_010603.6 | 71.7% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgcggccagc cgggccaacc cctacagcat cgtgtcatcg gaggaggacg 121 ggctgcacct ggtcaccatg tcgggcgcca acggcttcgg caacggcaag gtgcacacgc 181 ggcgcaggtg ccgcaaccgc ttcgtcaaga agaatggcca gtgcaacatt gagttcgcca 241 acatggacga gaagtcacag cgctacctgg ctgacatgtt caccacctgt gtggacatcc 301 gctggcggta catgctgctc atcttctcgc tggccttcct tgcctcctgg ctgctgttcg 361 gcatcatctt ctgggtcatc gcggtggcac acggtgacct ggagccggct gagggccggg 421 gccgcacacc ctgtgtgatg caggtgcacg gcttcatggc ggccttcctc ttctccatcg 481 agacgcagac caccatcggc tacgggctgc gctgtgtgac ggaggagtgc ccggtggccg 541 tcttcatggt ggtggcccag tccatcgtgg gctgcatcat cgactccttc atgattggtg 601 ccatcatggc caagatggca aggcccaaga agcgggcaca gacgctgctg ttcagccaca 661 acgccgtggt ggccctgcgt gacggcaagc tctgcctcat gtggcgtgtg ggtaacctgc 721 gcaagagcca cattgtggag gcccatgtgc gcgcgcagct catcaagccg cgggtcaccg 781 aggagggcga gtacatcccg ctggaccaga tcgacatcga tgtgggcttc gacaagggcc 841 tggaccgcat ctttctggtg tcgcccatca ccatcttgca tgagattgac gaggccagcc 901 cgctcttcgg catcagccgg caggacctgg agacggacga ctttgagatc gtggtcatcc 961 tggaaggcat ggtggaggcc acagccatga ccacccaggc ccgcagctcc tacctggcca 1021 atgagatcct gtggggtcac cgctttGAGC CCGTGCTCTT CGAGGAGAAG AACCAGTACA 1081 AGATTGACTA CTCGCACTTC CACAAGACCT ATGAGGTGCC CTCTACGCCC CGCTGCAGTG 1141 CGAAGGATCT GGTAGAGAAC AAGTTCCTGC TGCCCAGCGC CAACTCCTTC TGCTACGAGA 1201 ACGAGCTGGC CTTCCTGAGC CGTGACGAGG AGGATGAGGC GGACGGAGAC CAGGACGGCC 1261 GAAGCCGGGA CGGCCTCAGC CCCCAGGCCA GGCATGACTT TGACAGACTC CAGGCTGGCG 1321 GCGGGGTCCT GGAGCAGCGG CCCTACAGAC GGGAGTCAGA GATCTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA AACCCGACGG TCGGCATGCG TTCAACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt