Construct: ORF TRCN0000481129

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018077.1_s317c1
Derived from:
ccsbBroadEn_08557
DNA Barcode:
TCCAACTATCCCTTACAAGCGGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AMBRA1 (55626)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481129
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001367471.1 99.9% 99.8% 102A>G;3043C>G;3532C>G
2 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_017749.3 99.9% 99.8% 102A>G;3043C>G;3532C>G
3 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001267783.2 97.5% 97.4% (many diffs)
4 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001367468.1 92.9% 92.9% (many diffs)
5 human 55626 AMBRA1 autophagy and beclin 1 regu... XM_005253009.4 92.9% 92.9% (many diffs)
6 human 55626 AMBRA1 autophagy and beclin 1 regu... XM_006718259.2 92.9% 92.9% (many diffs)
7 human 55626 AMBRA1 autophagy and beclin 1 regu... XM_024448605.1 92.9% 92.9% (many diffs)
8 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001267782.2 92.7% 92.6% (many diffs)
9 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001367469.1 92.5% 92.4% (many diffs)
10 human 55626 AMBRA1 autophagy and beclin 1 regu... XM_005253011.4 90.7% 90.6% (many diffs)
11 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001300731.2 88.3% 88.2% (many diffs)
12 human 55626 AMBRA1 autophagy and beclin 1 regu... NM_001367470.1 85.1% 85% 0_1ins537;2506C>G;2995C>G
13 human 55626 AMBRA1 autophagy and beclin 1 regu... XM_024448606.1 76.4% 74.7% (many diffs)
14 human 55626 AMBRA1 autophagy and beclin 1 regu... XR_002957153.1 69.1% (many diffs)
15 human 55626 AMBRA1 autophagy and beclin 1 regu... NR_160027.1 62.5% (many diffs)
16 mouse 228361 Ambra1 autophagy/beclin 1 regulator 1 NM_001080754.1 91.5% 95.6% (many diffs)
17 mouse 228361 Ambra1 autophagy/beclin 1 regulator 1 XM_006499253.1 89.2% 93.2% (many diffs)
18 mouse 228361 Ambra1 autophagy/beclin 1 regulator 1 NM_172669.3 85.1% 88.9% (many diffs)
19 mouse 228361 Ambra1 autophagy/beclin 1 regulator 1 XM_006499252.1 82.9% 86.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3690
ORF length:
3624
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggttgtccca gaaaagaatg ctgtccggat actctggggg cgagaacggg
121gtgctcgggc catgggagct cagcggcttc tgcaggagct ggtagaggat aaaacccggt
181ggatgaaatg ggagggcaag agagtagaac tgccggatag tccacgctct accttcttat
241tggccttcag cccagacagg actctcttag cctccaccca tgtgaaccat aatatctata
301ttacggaggt gaagactggc aagtgtgttc attccctgat tggacaccgc cgtactccat
361ggtgtgtcac ttttcatccc accatctcag gccttattgc ttctggctgc ctagatgggg
421aggttaggat ttgggattta cacggtggca gtgaaagctg gttcacagat agcaacaatg
481ccattgcctc cctggctttc caccctacgg ctcagctcct gctgattgcc actgccaatg
541agatccactt ctgggactgg agtcgacggg aaccctttgc tgtggtgaag acagctagtg
601agatggaacg ggtccgtctg gtgagatttg atccacttgg acactactta ctcacagcaa
661ttgttaaccc ctctaatcaa cagggtgatg acgaaccaga gatccccata gatggaacag
721aattatccca ctaccgtcag cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc
781tcctccacaa tttcctgcac atgctgtcct cccgctcttc tggcatccag accgagccct
841tccatccccc ggagcaggcc tcgtcaacgc agcaggacca gggcctcctg aaccggccgt
901ctgccttcag tacagtccag agcagcactg ccggcaacac gctccgcaac ctcagtctgg
961gtcctacccg ccgctctttg ggagggcctc tgtctagcca cccttctagg tatcaccgag
1021aaatagctcc tgggttgaca ggatctgagt ggacccggac agtactcagt ctgaactccc
1081gctctgaggc ggaatccatg cccccgccca gaaccagtgc ctcttcggtg agtttgctgt
1141ctgtgctgag acagcaggaa ggtggctctc aggcatctgt gtacacttca gccacagaag
1201ggaggggttt tccggcatca gggttggcaa ctgagtcaga tggagggaat ggctccagcc
1261aaaacaactc gggcagcatt cgccatgagc ttcagtgtga cctgagacgc ttctttctgg
1321agtatgaccg gcttcaggag ctggatcaga gcctgagtgg ggaagctccc cagacccaac
1381aggcccagga aatgctcaac aataacattg aatctgagag gccaggccct tcccaccagc
1441ccaccccaca cagcagtgag aacaactcca acctgtcccg tggccacctg aatcgctgtc
1501gtgcttgcca caatctcctg accttcaaca acgataccct gcgctgggaa agaaccacac
1561ctaactactc ctctggcgag gctagttcct cttggcaggt ccccagctcc tttgagagtg
1621tgccatcaag tggcagccag ttgccacctc tcgagcggac tgagggccaa acgcccagct
1681ccagcaggct ggagttgagc agctctgcta gtccgcagga ggagaggact gtgggggtgg
1741cctttaacca ggagacaggc cactgggaaa gaatttacac ccagtccagc agatctggaa
1801ctgtgtcaca ggaggcctta catcaggata tgcctgagga gagctctgag gaggattcac
1861tcaggaggag gctgctggaa tcttccctca tttcattatc ccgttatgat ggagcaggat
1921ccagagagca cccaatttac ccagacccag cgagattatc tcctgctgca tactacgccc
1981agaggatgat ccagtatctc tcacggagag acagtattcg ccagcgctcc atgcgctacc
2041aacagaaccg tctccgttct tccacctcct cctcttcctc agacaaccag ggtccatcag
2101tagagggaac cgacttggaa tttgaggact ttgaggacaa tggtgacaga tccaggcacc
2161gagctccacg caatgcccgg atgtctgcac cttcgcttgg acgctttgtc ccaaggcgtt
2221tcttgctgcc tgagtacttg ccttatgctg ggatttttca tgaacgtgga cagcctggct
2281tggctactca ctcttctgtt aacagggtcc tggcaggggc agtgatcggt gatggacagt
2341ctgctgtggc cagtaacatt gccaatacta cctaccggct ccagtggtgg gacttcacta
2401agtttgacct ccctgaaatc agtaatgctt ccgtgaatgt gctggtgcag aactgcaaga
2461tctacaatga tgccagctgt gacatttctg cagatggcca gctcctggca gctttcatcc
2521ccagcagcca gaggggcttt cctgatgaag gcatcctggc agtgtactcc ctggcccccc
2581ataacctggg cgaaatgctc tacaccaagc gatttggtcc caatgccatt tcggtgagcc
2641tgtccccaat gggcagatat gtaatggtgg gcttggcctc acgaaggatc ctgctgcacc
2701cctccacaga gcacatggtg gcccaggtct tcaggctgca acaggcccat ggtggagaga
2761cctccatgag gagagttttc aacgtccttt atcccatgcc tgccgaccag cggagacatg
2821tcagtatcaa ctctgcccgt tggctgcctg agccagggct tggcttggcc tatggtacta
2881acaaaggaga cctggtgatc tgccgaccag aggccttaaa ctctggtgtt gagtactact
2941gggaccagct gaacgagacg gtcttcactg tccattccaa cagcaggagc agcgagcggc
3001ctggaaccag cagagccaca tggaggacag acagagacat ggggctgatg aatgccattg
3061ggcttcagcc ccggaaccct gccacctcag tgacatctca gggcaccgag actctggccc
3121ttcagctgca gaatgccgaa acacagactg agagggaggt gccggagcca gggacagccg
3181cctcaggtcc tggtgaaggt gagggttcag agtatggtgc cagtggagaa gatgcgctca
3241gcaggatcca gaggctgatg gcggagggcg gcatgacagc cgtggtgcag cgggagcaga
3301gcaccaccat ggccTCCATG GGCGGCTTCG GCAACAACAT CATCGTCAGC CACCGCATTC
3361ACCGCAGCTC TCAGACGGGC ACTGAACCTG GTGCCGCCCA CACCTCCTCA CCCCAGCCCT
3421CCACCTCTCG GGGACTGCTC CCAGAGGCCG GGCAACTGGC AGAGCGAGGC CTAAGCCCCC
3481GGACAGCTTC CTGGGACCAG CCTGGTACCC CTGGGCGGGA GCCAACCCAG CCAACCCTGC
3541CCTCTTCCTC CCCTGTCCCC ATTCCTGTTT CCCTTCCCAG CGCTGAGGGA CCAACCGTCC
3601ACTGCGAGTT GACCAATAAC AACCACCTTC TGGATGGTGG CAGCAGCAGG GGGGACGCTG
3661CAGGCCCTAG GGGAGAACCA CGGAACAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA
3721TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
3781CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCCAA
3841CTATCCCTTA CAAGCGGAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
3901tgaaagatt

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