Transcript: Mouse NM_172669.3

Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Ambra1 (228361)
Length:
5329
CDS:
362..4264

Additional Resources:

NCBI RefSeq record:
NM_172669.3
NBCI Gene record:
Ambra1 (228361)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172669.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000429045 ATGCTCTACACCAAGCGATTT pLKO_005 3164 CDS 100% 10.800 15.120 N AMBRA1 n/a
2 TRCN0000189704 GCATTCGCCATGAGCTTCAAT pLKO.1 1845 CDS 100% 5.625 7.875 N Ambra1 n/a
3 TRCN0000190777 GCACTGCAATGTGACCAATAA pLKO.1 4168 CDS 100% 13.200 10.560 N Ambra1 n/a
4 TRCN0000189905 GCCACTGGGAACGCATTTATA pLKO.1 2328 CDS 100% 15.000 10.500 N Ambra1 n/a
5 TRCN0000189940 GCACCTTCACTTGGACGATTT pLKO.1 2756 CDS 100% 10.800 7.560 N Ambra1 n/a
6 TRCN0000168355 GAGATTATCTCCTGCTGCATA pLKO.1 2521 CDS 100% 4.950 3.465 N AMBRA1 n/a
7 TRCN0000167238 CCTCATTTCATTATCCCGTTA pLKO.1 2455 CDS 100% 4.050 2.835 N AMBRA1 n/a
8 TRCN0000190629 GCACAGTACAAGATTCTGCTA pLKO.1 1140 CDS 100% 2.640 1.848 N Ambra1 n/a
9 TRCN0000425163 GTGACCTGAGACGCTTCTTTC pLKO_005 1866 CDS 100% 10.800 7.560 N AMBRA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172669.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08557 pDONR223 100% 85.1% 88.9% None (many diffs) n/a
2 ccsbBroad304_08557 pLX_304 0% 85.1% 88.9% V5 (many diffs) n/a
3 TRCN0000481129 TCCAACTATCCCTTACAAGCGGAA pLX_317 10.5% 85.1% 88.9% V5 (many diffs) n/a
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