Construct: ORF TRCN0000481151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004069.1_s317c1
- Derived from:
- ccsbBroadEn_06398
- DNA Barcode:
- GGTAATGCAGGCCTGACCACTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPN (3249)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481151
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3249 | HPN | hepsin | NM_002151.2 | 99.9% | 100% | 609C>T |
| 2 | human | 3249 | HPN | hepsin | NM_182983.2 | 99.9% | 100% | 609C>T |
| 3 | human | 3249 | HPN | hepsin | XM_005258838.4 | 99.9% | 100% | 609C>T |
| 4 | human | 3249 | HPN | hepsin | XM_017026731.1 | 99.9% | 100% | 609C>T |
| 5 | human | 3249 | HPN | hepsin | XM_017026732.1 | 88.1% | 81.6% | (many diffs) |
| 6 | mouse | 15451 | Hpn | hepsin | NM_008281.4 | 84.6% | 88.4% | (many diffs) |
| 7 | mouse | 15451 | Hpn | hepsin | NM_001110252.2 | 81% | 83.7% | (many diffs) |
| 8 | mouse | 15451 | Hpn | hepsin | NM_001276269.1 | 79.4% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagaaggag ggtggccgga ctgtgccatg ctgctccaga cccaaggtgg 121 cagctctcac tgcggggacc ctgctacttc tgacagccat cggggcggca tcctgggcca 181 ttgtggctgt tctcctcagg agtgaccagg agccgctgta cccagtgcag gtcagctctg 241 cggacgctcg gctcatggtc tttgacaaga cggaagggac gtggcggctg ctgtgctcct 301 cgcgctccaa cgccagggta gccggactca gctgcgagga gatgggcttc ctcagggcac 361 tgacccactc cgagctggac gtgcgaacgg cgggcgccaa tggcacgtcg ggcttcttct 421 gtgtggacga ggggaggctg ccccacaccc agaggctgct ggaggtcatc tccgtgtgtg 481 attgccccag aggccgtttc ttggccgcca tctgccaaga ctgtggccgc aggaagctgc 541 ccgtggaccg catcgtggga ggccgggaca ccagcttggg ccggtggccg tggcaagtca 601 gccttcgcta tgatggagca cacctctgtg ggggatccct gctctccggg gactgggtgc 661 tgacagccgc ccattgcttc ccggagcgga accgggtcct gtcccgatgg cgagtgtttg 721 ccggtgccgt ggcccaggcc tctccccacg gtctgcagct gggggtgcag gctgtggtct 781 accacggggg ctatcttccc tttcgggacc ccaacagcga ggagaacagc aacgatattg 841 ccctggtcca cctctccagt cccctgcccc tcacagaata catccagcct gtgtgcctcc 901 cagctgccgg ccaggcccTG GTGGATGGCA AGATCTGTAC CGTGACGGGC TGGGGCAACA 961 CGCAGTACTA TGGCCAACAG GCCGGGGTAC TCCAGGAGGC TCGAGTCCCC ATAATCAGCA 1021 ATGATGTCTG CAATGGCGCT GACTTCTATG GAAACCAGAT CAAGCCCAAG ATGTTCTGTG 1081 CTGGCTACCC CGAGGGTGGC ATTGATGCCT GCCAGGGCGA CAGCGGTGGT CCCTTTGTGT 1141 GTGAGGACAG CATCTCTCGG ACGCCACGTT GGCGGCTGTG TGGCATTGTG AGTTGGGGCA 1201 CTGGCTGTGC CCTGGCCCAG AAGCCAGGCG TCTACACCAA AGTCAGTGAC TTCCGGGAGT 1261 GGATCTTCCA GGCCATAAAG ACTCACTCCG AAGCCAGCGG CATGGTGACC CAGCTCTGCC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAGGTAATGC AGGCCTGACC ACTTTCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt