Construct: ORF TRCN0000481171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008212.2_s317c1
- Derived from:
- ccsbBroadEn_12052
- DNA Barcode:
- GTGGTGGAGGGGTATGGGCTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR44 (54521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54521 | WDR44 | WD repeat domain 44 | XM_017029600.2 | 99.8% | 99.8% | 16A>G;603C>T;1851T>C |
| 2 | human | 54521 | WDR44 | WD repeat domain 44 | XM_011531353.3 | 70% | 70% | (many diffs) |
| 3 | human | 54521 | WDR44 | WD repeat domain 44 | NM_019045.5 | 68.1% | 68.1% | (many diffs) |
| 4 | human | 54521 | WDR44 | WD repeat domain 44 | NM_001184965.2 | 67.2% | 67.2% | (many diffs) |
| 5 | human | 54521 | WDR44 | WD repeat domain 44 | NM_001184966.1 | 62.8% | 62.8% | (many diffs) |
| 6 | mouse | 72404 | Wdr44 | WD repeat domain 44 | XM_011247497.2 | 93.1% | 96.9% | (many diffs) |
| 7 | mouse | 72404 | Wdr44 | WD repeat domain 44 | NM_001305675.1 | 63.4% | 66% | (many diffs) |
| 8 | mouse | 72404 | Wdr44 | WD repeat domain 44 | NM_175180.3 | 63.4% | 66% | (many diffs) |
| 9 | mouse | 72404 | Wdr44 | WD repeat domain 44 | XM_006527700.3 | 63.4% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1935
- ORF length:
- 1869
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcagaaagt gcggttaagg attctcagcc ttctcttgat ttggcaagtg 121 ctaccagtgg agataaaata gttaccgccc aggaaaatgg aaaagcacct gatgggcaga 181 ctgtagcagg tgaagtgatg ggccctcaga gacctagatc caactctggg agagagctta 241 ctgatgagga aattttagcc agtgtaatga ttaagaacct ggatactgga gaagaaatac 301 ctttgagtct tgcagaagag aaactaccaa caggcattaa tcctctcact ctacacatca 361 tgagaaggac aaaagaatat gtaagtaatg acgcggcaca gtcagatgat gaagagaagt 421 tacagtctca gccaacagat actgatggtg gaaggttaaa acagaaaacg actcaactaa 481 agaagtttct tggaaaatca gtaaagagag caaagcacct tgctgaggaa tatggtgaac 541 gtgctataaa taaagttaaa agtgttagag atgaagtgtt tcatactgat caagatgatc 601 cttcatcaag tgatgatgaa ggaatgccat acacaagacc agttaaattc aaagcagcac 661 acggttttaa aggaccttat gattttgatc agatcaaagt ggtgcaagat cttagtggtg 721 aacatatggg agctgtttgg accatgaaat tttctcactg tggccgatta cttgcctcag 781 ctggacaaga caatgtagtg agaatatggg ctttaaaaaa tgcttttgac tatttcaaca 841 atatgcgaat gaaatacaat actgaaggac gtgtgtcccc atcaccctct caggaaagtc 901 taagttcatc aaaatcggat acagatacag gggtatgcag tggaactgat gaagaccctg 961 atgataaaaa cgcacccttt cggcaacggc cattttgcaa atataaagga catactgctg 1021 atctccttga tctttcatgg tctaaaaact actttcttct ttcttcttca atggataaaa 1081 cagtcagatt atggcacatt tctcgaagag aatgcctttg ctgttttcaa catatagatt 1141 ttgtcactgc catagctttt catccaagag atgacaggta ttttctaagt gggtctttgg 1201 atggaaagct ccgcctttgg aacatacctg acaaaaaagt ggctttgtgg aatgaagtag 1261 atggtcagac aaaattgatc acagctgcaa atttctgtca gaatggcaaa tatgcagtga 1321 ttgggacata tgatggcaga tgtattttct atgatacaga gcatttgaaa taccatacac 1381 aaatacatgt ccgatctact agagggcgca acaaggttgg aagaaaaatt actggcattg 1441 agcctttacc tggagaaaat aagatattgg taaccTCAAA TGACTCCAGA ATCAGACTAT 1501 ATGATTTGAG AGATTTGTCA CTATCCATGA AGTATAAGGG TTACGTCAAT AGCAGCAGCC 1561 AGATCAAAGC AAGTTTCAGC CATGATTTTA CTTACCTCGT TAGTGGTTCA GAAGATAAGT 1621 ATGTTTATAT CTGGAGTACC TACCATGACC TAAGCAAGTT TACTTCAGTC AGGAGAGATC 1681 GTAATGACTT CTGGGAAGGT ATTAAAGCCC ACAATGCAGT TGTTACATCA GCCATCTTTG 1741 CACCAAACCC AAGTTTGATG TTATCTTTGG ATGTGCAATC TGAAAAATCA GAAGGGAACG 1801 AGAAAAGTGA AGATGCTGAA GTTTTGGATG CCACACCTTC TGGTATTATG AAGACAGATA 1861 ACACAGAAGT TCTTCTCTCT GCTGACTTCA CTGGAGCAAT CAAAGTGTTT GTTAACAAAA 1921 GAAAAAATGT ATCTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1981 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2041 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTGGTGGAGG GGTATGGGCT 2101 CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt