Construct: ORF TRCN0000481180

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003427.1_s317c1
Derived from:
ccsbBroadEn_06380
DNA Barcode:
TCCAGTAACTAACAAGCTTGTCTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HLCS (3141)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481180
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3141 HLCS holocarboxylase synthetase NM_000411.8 99.9% 100% 834C>T
2 human 3141 HLCS holocarboxylase synthetase NM_001242784.3 99.9% 100% 834C>T
3 human 3141 HLCS holocarboxylase synthetase NM_001242785.2 99.9% 100% 834C>T
4 human 3141 HLCS holocarboxylase synthetase NM_001352515.2 99.9% 100% 834C>T
5 human 3141 HLCS holocarboxylase synthetase NM_001352516.2 99.9% 100% 834C>T
6 human 3141 HLCS holocarboxylase synthetase NM_001352517.1 99.9% 100% 834C>T
7 human 3141 HLCS holocarboxylase synthetase NM_001352518.1 99.9% 100% 834C>T
8 human 3141 HLCS holocarboxylase synthetase XM_006723995.1 99.9% 100% 834C>T
9 human 3141 HLCS holocarboxylase synthetase XM_011529538.1 99.9% 100% 834C>T
10 human 3141 HLCS holocarboxylase synthetase XM_011529539.3 99.9% 100% 834C>T
11 human 3141 HLCS holocarboxylase synthetase XM_017028330.1 99.9% 100% 834C>T
12 human 3141 HLCS holocarboxylase synthetase XM_024452065.1 92.1% 92.1% 0_1ins171;663C>T
13 human 3141 HLCS holocarboxylase synthetase XM_024452066.1 92.1% 92.1% 0_1ins171;663C>T
14 human 3141 HLCS holocarboxylase synthetase NM_001352514.2 83.1% 83.1% 1_441del;1275C>T
15 human 3141 HLCS holocarboxylase synthetase XR_001754840.1 78.1% (many diffs)
16 human 3141 HLCS holocarboxylase synthetase XR_001754837.2 66.1% (many diffs)
17 human 3141 HLCS holocarboxylase synthetase XM_011529540.2 55.7% 55.4% (many diffs)
18 human 3141 HLCS holocarboxylase synthetase XR_001754835.1 43.1% (many diffs)
19 human 3141 HLCS holocarboxylase synthetase XR_001754836.1 41.8% (many diffs)
20 human 3141 HLCS holocarboxylase synthetase NR_148020.2 26.1% (many diffs)
21 human 3141 HLCS holocarboxylase synthetase NR_148021.1 25.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2244
ORF length:
2178
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agatagactc cacatggata atggactggt accccaaaag attgtgtcgg
121tgcacttgca ggactccact ctgaaggaag ttaaggatca ggtctcaaac aagcaagccc
181agatcctaga gccgaagcct gaaccttctc ttgagattaa gcctgagcag gacggtatgg
241agcatgttgg cagagatgac ccaaaggctc ttggtgaaga acccaaacaa aggagaggca
301gtgcctctgg gagtgagcct gctggggaca gtgacagggg agggggcccc gttgagcatt
361atcacctcca tctgtctagt tgccacgagt gtctggaact tgagaacagc accattgagt
421cagtcaagtt tgcgtctgcc gagaacattc cagaccttcc ctacgattat agcagcagtt
481tggagagtgt tgctgatgag acctcccccg aaagagaagg gaggagagtc aacctcacgg
541gaaaggcacc caacatcctc ctctatgtgg gctccgactc ccaggaagcc ctcggccggt
601tccacgaggt ccggtctgtg ctggccgact gtgtggacat tgacagttat attctctacc
661acctgctgga ggacagtgct ctcagagacc cgtggacgga caactgtctg ctgttggtca
721ttgctaccag ggagtccatt cccgaagacc tgtaccagaa gttcatggcc tatctttctc
781agggagggaa ggtgttgggc ctgtcttcat ccttcacctt tggtggcttt caggtgacaa
841gcaagggtgc actgcacaag acagtccaga acttggtttt ctccaaggct gaccagagtg
901aggtgaagct cagcgtcttg agcagtggct gcaggtacca ggaaggcccc gtccggctca
961gccccggcag gctccagggc cacctggaga atgaggacaa ggacaggatg attgtgcatg
1021tgccttttgg aactcgcggg ggagaagctg ttctttgcca ggtgcactta gaactacctc
1081ccagctccaa catagtgcaa actccagaag attttaactt gctcaagtca agcaatttta
1141gaagatacga agtccttaga gagattctga caacccttgg cctcagctgt gacatgaaac
1201aagttcctgc cttaactcct ctttacttgc tgtcagctgc ggaggaaatc agggatcctc
1261ttatgcagtg gcttgggaaa catgtggact ccgagggaga aataaaatcc ggccagctct
1321ctcttagatt tgtttcatcc tacgtgtctg aagtagaaat aaccccatct tgtatacctg
1381tggtgaccaa catggaggcc ttctcatcag aacatttcaa cttagagatc tatcgccaaa
1441atctgcagac caagcagttg gggaaagtaa ttttgtttgc cgaagtgacc cccacaacga
1501tgcgtctcct ggatgggctg atgtttcaga caccgcagga aatgggctta atagtgatcg
1561cggcccggca gaccgagggc aaaggacggg gagggaatgt gtggctgagc cctgtgggat
1621gtgctctttc tactctgctc atctccattc cactgagatc ccagctggga cagaggatcc
1681cgtttgtcca gcatctgatg tccgtggctg tcgtggaagc agtgaggtcc attcccgagt
1741atcaggatat caacttacga gtgaagtggc ccaacgatat ttattacagt gaccTCATGA
1801AGATCGGCGG AGTTCTGGTT AACTCAACAC TCATGGGAGA AACATTTTAT ATACTTATTG
1861GCTGTGGATT TAATGTGACT AACAGTAACC CTACCATCTG CATCAACGAC CTCATCACAG
1921AATACAATAA ACAACACAAG GCAGAACTGA AGCCCTTAAG AGCCGATTAT CTCATCGCCA
1981GAGTCGTGAC TGTGCTGGAG AAACTGATCA AAGAGTTTCA GGACAAAGGG CCCAACAGCG
2041TCCTTCCCCT TTATTACCGA TACTGGGTCC ACAGTGGTCA GCAAGTCCAT CTGGGCAGCG
2101CAGAGGGACC AAAGGTGTCC ATCGTTGGCC TGGACGATTC TGGCTTCCTC CAGGTTCACC
2161AGGAGGGCGG CGAGGTTGTG ACTGTGCACC CGGACGGCAA CTCCTTCGAC ATGCTGAGAA
2221ACCTCATCCT CCCCAAACGG CGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2281AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2341TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCAGTAACTA
2401ACAAGCTTGT CTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2461att

Download FASTA (ORF) (Full)

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