Construct: ORF TRCN0000481202

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007459.1_s317c1
Derived from:
ccsbBroadEn_06103
DNA Barcode:
TACATTCTGGCATTCTTTAAGCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DMD (1756)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481202
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1756 DMD dystrophin NM_004016.3 99.9% 100% 768C>T
2 human 1756 DMD dystrophin NM_004018.3 97.9% 97.9% 768C>T;1018_1019ins39
3 human 1756 DMD dystrophin NM_004015.3 96.4% 95.4% (many diffs)
4 human 1756 DMD dystrophin NM_004017.3 94.3% 93.3% (many diffs)
5 human 1756 DMD dystrophin NM_004014.2 62.6% 61.6% (many diffs)
6 human 1756 DMD dystrophin NM_004019.3 53.4% 53.5% 768C>T;1020_1020delGins886
7 human 1756 DMD dystrophin NM_004021.3 50.8% 50.6% (many diffs)
8 human 1756 DMD dystrophin NM_004022.2 49.8% 49.5% (many diffs)
9 human 1756 DMD dystrophin NM_004013.2 49.1% 48.2% (many diffs)
10 human 1756 DMD dystrophin NM_004023.3 42% 41.7% (many diffs)
11 human 1756 DMD dystrophin NM_004020.3 40.2% 39.4% (many diffs)
12 human 1756 DMD dystrophin XM_017029331.1 35.9% 35.7% (many diffs)
13 human 1756 DMD dystrophin NM_004012.4 25.8% 25.4% (many diffs)
14 human 1756 DMD dystrophin NM_004011.4 25.8% 25.4% (many diffs)
15 human 1756 DMD dystrophin XM_006724473.2 17.3% 17.1% (many diffs)
16 human 1756 DMD dystrophin XM_011545467.1 17.2% 17.1% (many diffs)
17 human 1756 DMD dystrophin XM_006724469.3 17.1% 17% (many diffs)
18 human 1756 DMD dystrophin XM_006724468.2 17% 16.9% (many diffs)
19 human 1756 DMD dystrophin NM_004010.3 17% 16.7% (many diffs)
20 human 1756 DMD dystrophin XM_006724470.3 16.7% 16.6% (many diffs)
21 human 1756 DMD dystrophin NM_000109.4 16.5% 16.2% (many diffs)
22 human 1756 DMD dystrophin NM_004009.3 16.5% 16.2% (many diffs)
23 human 1756 DMD dystrophin NM_004006.2 16.5% 16.2% (many diffs)
24 human 1756 DMD dystrophin XM_017029328.1 16.1% 15.8% (many diffs)
25 human 1756 DMD dystrophin XM_006724474.3 14.1% 14% (many diffs)
26 human 1756 DMD dystrophin XM_006724475.2 13.5% 13.2% (many diffs)
27 human 1756 DMD dystrophin XM_011545468.2 5.9% 5.4% (many diffs)
28 mouse 13405 Dmd dystrophin, muscular dystrophy NM_001314034.1 94.4% 99.6% (many diffs)
29 mouse 13405 Dmd dystrophin, muscular dystrophy NM_001314035.1 92.3% 97.6% (many diffs)
30 mouse 13405 Dmd dystrophin, muscular dystrophy NM_001314036.1 90.7% 95.1% (many diffs)
31 mouse 13405 Dmd dystrophin, muscular dystrophy NM_001314037.1 88.7% 93% (many diffs)
32 mouse 13405 Dmd dystrophin, muscular dystrophy XM_006527773.1 58.1% 60.6% (many diffs)
33 mouse 13405 Dmd dystrophin, muscular dystrophy NM_001314038.1 49.9% 53.3% (many diffs)
34 mouse 13405 Dmd dystrophin, muscular dystrophy XM_017318375.1 16.3% 17.1% (many diffs)
35 mouse 13405 Dmd dystrophin, muscular dystrophy XM_006527768.3 16.2% 17% (many diffs)
36 mouse 13405 Dmd dystrophin, muscular dystrophy XM_006527767.3 16.1% 16.9% (many diffs)
37 mouse 13405 Dmd dystrophin, muscular dystrophy XM_017318373.1 15.8% 16.6% (many diffs)
38 mouse 13405 Dmd dystrophin, muscular dystrophy NM_007868.6 15.5% 16.2% (many diffs)
39 mouse 13405 Dmd dystrophin, muscular dystrophy XM_017318374.1 15.2% 15.8% (many diffs)
40 mouse 13405 Dmd dystrophin, muscular dystrophy XM_017318376.1 13.2% 14% (many diffs)
41 mouse 13405 Dmd dystrophin, muscular dystrophy XM_017318377.1 12.6% 13.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1971
ORF length:
1905
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ggaacagctc aaaggccacg agactcaaac aacttgctgg gaccatccca
121aaatgacaga gctctaccag tctttagctg acctgaataa tgtcagattc tcagcttata
181ggactgccat gaaactccga agactgcaga aggccctttg cttggatctc ttgagcctgt
241cagctgcatg tgatgccttg gaccagcaca acctcaagca aaatgaccag cccatggata
301tcctgcagat tattaattgt ttgaccacta tttatgaccg cctggagcaa gagcacaaca
361atttggtcaa cgtccctctc tgcgtggata tgtgtctgaa ctggctgctg aatgtttatg
421atacgggacg aacagggagg atccgtgtcc tgtcttttaa aactggcatc atttccctgt
481gtaaagcaca tttggaagac aagtacagat accttttcaa gcaagtggca agttcaacag
541gattttgtga ccagcgcagg ctgggcctcc ttctgcatga ttctatccaa attccaagac
601agttgggtga agttgcatcc tttgggggca gtaacattga gccaagtgtc cggagctgct
661tccaatttgc taataataag ccagagatcg aagcggccct cttcctagac tggatgagac
721tggaacccca gtccatggtg tggctgcccg tcctgcacag agtggctgct gcagaaactg
781ccaagcatca ggccaaatgt aacatctgca aagagtgtcc aatcattgga tttaggtaca
841ggagtctaaa gcactttaat tatgacatct gccaaagctg ctttttttct ggtcgagttg
901caaaaggcca taaaatgcac tatcccatgg tggaatattg cactccgact acatcaggag
961aagatgttcg agactttgcc aaggtactaa aaaacaaatt tcgaaccaaa aggtattttg
1021cgaagcatcc ccgaatgggc tacctgccag tgcagactgt cttagagggg gacaacatgg
1081aaactcccgt tactctgatc aacttctggc cagtagattc tgcgcctgcc tcgtcccctc
1141agctttcaca cgatgatact cattcacgca ttgaacatta tgctagcagg ctagcagaaa
1201tggaaaacag caatggatct tatctaaatg atagcatctc tcctaatgag agcatagatg
1261atgaacattt gttaatccag cattactgcc aaagtttgaa ccaggactcc cccctgagcc
1321agcctcgtag tcctgcccag atcttgattt ccttagagag tgaggaaaga ggggagctag
1381agagaatcct agcagatctt gaggaagaaa acaggaatct gcaagcagaa tatgaccgtc
1441taaagcagca gcacgaacat aaaggcctgt ccccactgcc gtcccctcct gaaatgatgc
1501ccacctctcc ccAGAGTCCC CGGGATGCTG AGCTCATTGC TGAGGCCAAG CTACTGCGTC
1561AACACAAAGG CCGCCTGGAA GCCAGGATGC AAATCCTGGA AGACCACAAT AAACAGCTGG
1621AGTCACAGTT ACACAGGCTA AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAAGTGA
1681ATGGCACAAC GGTGTCCTCT CCTTCTACCT CTCTACAGAG GTCCGACAGC AGTCAGCCTA
1741TGCTGCTCCG AGTGGTTGGC AGTCAAACTT CGGACTCCAT GGGTGAGGAA GATCTTCTCA
1801GTCCTCCCCA GGACACAAGC ACAGGGTTAG AGGAGGTGAT GGAGCAACTC AACAACTCCT
1861TCCCTAGTTC AAGAGGACAC AATGTAGGAA GTCTTTTCCA CATGGCAGAT GATTTGGGCA
1921GAGCGATGGA GTCCTTAGTA TCAGTCATGA CAGATGAAGA AGGAGCAGAA TACCCAACTT
1981TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2041CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2101CTTGTGGAAA GGACGATACA TTCTGGCATT CTTTAAGCAA ACGCGTTAAG TCgacaatca
2161acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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