Construct: ORF TRCN0000481255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005471.2_s317c1
- Derived from:
- ccsbBroadEn_15052
- DNA Barcode:
- ACTCATTCTGAACGTGCAGCACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME7 (29922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_013330.5 | 99.9% | 100% | 207T>C |
| 2 | human | 29922 | NME7 | NME/NM23 family member 7 | NM_197972.2 | 90.3% | 90.4% | 0_1ins108;99T>C |
| 3 | human | 29922 | NME7 | NME/NM23 family member 7 | NR_104229.1 | 65.5% | (many diffs) | |
| 4 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_001293313.1 | 81.1% | 84% | (many diffs) |
| 5 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_138314.4 | 81.1% | 84% | (many diffs) |
| 6 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496692.2 | 77.2% | 79.7% | (many diffs) |
| 7 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | XM_006496693.3 | 77.2% | 79.7% | (many diffs) |
| 8 | mouse | 171567 | Nme7 | NME/NM23 family member 7 | NM_178071.6 | 55.9% | 56.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcatagtgaa agattcgttt tcattgcaga gtggtatgat ccaaatgctt 121 cacttcttcg acgttatgag cttttatttt acccagggga tggatctgtt gaaatgcatg 181 atgtaaagaa tcatcgcacc tttttaaagc ggaccaaata tgataacctg cacttggaag 241 atttatttat aggcaacaaa gtgaatgtct tctctcgaca actggtatta attgactatg 301 gggatcaata tacagctcgc cagctgggca gtaggaaaga aaaaacgcta gccctaatta 361 aaccagatgc aatatcaaag gctggagaaa taattgaaat aataaacaaa gctggattta 421 ctataaccaa actcaaaatg atgatgcttt caaggaaaga agcattggat tttcatgtag 481 atcaccagtc aagacccttt ttcaatgagc tgatccagtt tattacaact ggtcctatta 541 ttgccatgga gattttaaga gatgatgcta tatgtgaatg gaaaagactg ctgggacctg 601 caaactctgg agtggcacgc acagatgctt ctgaaagcat tagagccctc tttggaacag 661 atggcataag aaatgcagcg catggccctg attcttttgc ttctgcggcc agagaaatgg 721 agttgttttt tccttcaagt ggaggttgtg ggccggcaaa cactgctaaa tttactaatt 781 gtaCCTGTTG CATTGTTAAA CCCCATGCTG TCAGTGAAGG ACTGTTGGGA AAGATCCTGA 841 TGGCTATCCG AGATGCAGGT TTTGAAATCT CAGCTATGCA GATGTTCAAT ATGGATCGGG 901 TTAATGTTGA GGAATTCTAT GAAGTTTATA AAGGAGTAGT GACCGAATAT CATGACATGG 961 TGACAGAAAT GTATTCTGGC CCTTGTGTAG CAATGGAGAT TCAACAGAAT AATGCTACAA 1021 AGACATTTCG AGAATTTTGT GGACCTGCTG ATCCTGAAAT TGCCCGGCAT TTACGCCCTG 1081 GAACTCTCAG AGCAATCTTT GGTAAAACTA AGATCCAGAA TGCTGTTCAC TGTACTGATC 1141 TGCCAGAGGA TGGCCTATTA GAGGTTCAAT ACTTCTTCAA GATCTTGGAT AATTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAA CTCATTCTGA ACGTGCAGCA CGGACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att