Construct: ORF TRCN0000481270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015744.1_s317c1
- Derived from:
- ccsbBroadEn_15524
- DNA Barcode:
- CGAAACCAGAAGAAAAAACATGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFKL (5211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_002626.6 | 99.9% | 100% | 366C>T |
| 2 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261135.3 | 97% | 97.1% | 159_227del;435C>T |
| 3 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028369.1 | 96.8% | 96.5% | (many diffs) |
| 4 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261137.4 | 96.2% | 96.2% | 0_1ins87;279C>T |
| 5 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028370.1 | 96.2% | 96.2% | 0_1ins87;279C>T |
| 6 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028372.1 | 96.2% | 96.2% | 0_1ins87;279C>T |
| 7 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_001002021.3 | 92.2% | 91.4% | (many diffs) |
| 8 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_024452085.1 | 92.2% | 91.4% | (many diffs) |
| 9 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_006724011.1 | 89.7% | 88.9% | (many diffs) |
| 10 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028368.1 | 89.7% | 88.9% | (many diffs) |
| 11 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_011529603.2 | 70.3% | 67.9% | (many diffs) |
| 12 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | NM_008826.4 | 87.1% | 94.2% | (many diffs) |
| 13 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | XM_017313846.1 | 52.2% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2406
- ORF length:
- 2340
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcggtggac ctggagaagc tgcgggcgtc gggcgcgggc aaggccatcg 121 gcgtcctgac cagcggcggc gacgcgcaag gcatgaacgc tgctgtccgg gctgtgacgc 181 gcatgggcat ttatgtgggt gccaaagtct tcctcatcta cgagggctat gagggcctcg 241 tggagggagg tgagaacatc aagcaggcca actggctgag cgtctccaac atcatccagc 301 tgggcggcac tatcattggc agcgctcgct gcaaggcctt taccaccagg gaggggcgcc 361 gggcagcggc ctacaacctg gtccagcacg gcatcaccaa cctgtgcgtc atcggcgggg 421 atggcagcct tacaggtgcc aacatcttcc gcagcgagtg gggcagcctg ctggaggagc 481 tggtggcgga aggtaagatc tcagagacta cagcccggac ctactcgcac ctgaacatcg 541 cgggcctagt gggctccatc gataacgact tctgcggcac cgacatgacc atcggcacgg 601 actcggccct ccaccgcatc atggaggtca tcgatgccat caccaccact gcccagagcc 661 accagaggac cttcgtgctg gaagtgatgg gccggcactg cgggtacctg gcgctggtat 721 ctgcactggc ctcaggggcc gactggctgt tcatccccga ggctccaccc gaggacggct 781 gggagaactt catgtgtgag aggctgggtg agactcggag ccgtgggtcc cgactgaaca 841 tcatcatcat cgctgagggt gccattgacc gcaacgggaa gcccatctcg tccagctacg 901 tgaaggacct ggtggttcag aggctgggct tcgacacccg tgtaactgtg ctgggccacg 961 tgcagcgggg agggacgccc tctgccttcg accggatcct gagcagcaag atgggcatgg 1021 aggcggtgat ggcgctgctg gaagccacgc ctgacacgcc ggcctgcgtg gtcaccctct 1081 cggggaacca gtcagtgcgg ctgcccctca tggagtgcgt gcagatgacc aaggaagtgc 1141 agaaagccat ggatgacaag aggtttgacg aggccaccca gctccgtggt gggagcttcg 1201 agaacaactg gaacatttac aagctcctcg cccaccagaa gccccccaag gagaagtcta 1261 acttctccct ggccatcctg aatgtggggg ccccggcggc tggcatgaat gcggccgtgc 1321 gctcggcggt gcggaccggc atctcccatg gacacacagt atacgtggtg cacgatggct 1381 tcgaaggcct agccaagggt caggtgcaag aagtaggctg gcacgacgtg gccggctggt 1441 tggggcgtgg tggctccatg ctggggacca agaggaccct gcccaagggc cagctggagt 1501 ccattgtgga gaacatccgc atctatggta ttcacgccct gctggtggtc ggtgggtttg 1561 aggcctatga aggggtgctg cagctggtgg aggctcgcgg gcgctacgag gagctctgca 1621 tcgtcatgtg tgtcatccca gccaccatca gcaacaacgt ccctggcacc gacttcagcc 1681 tgggctccga cactgctgta aatgccgcca tggagagctg tgaccgcatc aaacagtctg 1741 cctcggggac caagcgccgt gtgttcatcg tggagaccat ggggggttac tgtggctacc 1801 tggccaccgt gactggcatt gctgtggggg ccgacgccgc ctacgtcttc gaggaccctt 1861 tcaacatcca cgacttaaag gTCAACGTGG AGCACATGAC GGAGAAGATG AAGACAGACA 1921 TTCAGAGGGG CCTGGTGCTG CGGAACGAGA AGTGCCATGA CTACTACACC ACGGAGTTCC 1981 TGTACAACCT GTACTCATCA GAGGGCAAGG GCGTCTTCGA CTGCAGGACC AATGTCCTGG 2041 GCCACCTGCA GCAGGGTGGC GCTCCAACCC CCTTTGACCG GAACTATGGG ACCAAGCTGG 2101 GGGTGAAGGC CATGCTGTGG TTGTCGGAGA AGCTGCGCGA GGTTTACCGC AAGGGACGGG 2161 TGTTCGCCAA TGCCCCAGAC TCGGCCTGCG TGATCGGCCT GAAGAAGAAG GCGGTGGCCT 2221 TCAGCCCCGT CACTGAGCTC AAGAAAGACA CTGATTTCGA GCACCGCATG CCACGGGAGC 2281 AGTGGTGGCT GAGCCTGCGG CTCATGCTGA AGATGCTGGC ACAATACCGC ATCAGTATGG 2341 CCGCCTACGT GTCAGGGGAG CTGGAGCACG TGACCCGCCG CACCCTGAGC ATGGACAAGG 2401 GCTTCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2461 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2521 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGAAACCAG AAGAAAAAAC ATGACACGCG 2581 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt