Construct: ORF TRCN0000481283

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008625.2_s317c1
Derived from:
ccsbBroadEn_03899
DNA Barcode:
GGAACTAAGGTCCTCACAGTTAAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ZFAND3 (60685)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481283
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60685 ZFAND3 zinc finger AN1-type contai... NM_021943.3 100% 100%
2 human 60685 ZFAND3 zinc finger AN1-type contai... XM_005249269.2 90.3% 89.8% 295_296ins66
3 human 60685 ZFAND3 zinc finger AN1-type contai... XM_017011171.2 86.6% 84.9% (many diffs)
4 human 60685 ZFAND3 zinc finger AN1-type contai... XM_011514790.2 75.3% 75.3% 360_361ins168
5 mouse 21769 Zfand3 zinc finger, AN1-type domain 3 XM_006524027.1 92.2% 95.5% (many diffs)
6 mouse 21769 Zfand3 zinc finger, AN1-type domain 3 NM_148926.2 82.9% 85.9% (many diffs)
7 mouse 21769 Zfand3 zinc finger, AN1-type domain 3 XM_006524028.2 82.8% 85% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
747
ORF length:
681
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttgccatggg agacgctggg agcgagcgca gcaaagcgcc cagcctgccg cctcgctgtc
121cctgcggctt ctgggggtcc agcaagacta tgaatctctg ttccaaatgc tttgctgatt
181ttcaaaagaa acagccagac gatgattccg ctccaagtac aagtaacagc caatcagatt
241tgttttccga agagaccacc agtgacaaca acaatacctc gataaccacg ccaactctta
301gtcccagcca gcagccgctt ccgacagaac tgaatgtaac ttcaccgagt aaagaggagt
361gtgggccatg cacagacaca gctcatgtct cattaatcac accaacaaaa agatcctgtg
421gtacagattc acagtctgag aatgaggctt caccagtaaa acggccacga ctacttgaga
481atacggaacg gtccgaggaa accagtcgat ctaaacagaa gagtcgacgt cggtgcttcc
541agtgccaaac caaactGGAG CTGGTGCAGC AGGAATTGGG ATCGTGTCGC TGCGGTTATG
601TGTTCTGTAT GTTACATCGC CTCCCCGAGC AGCACGACTG CACATTCGAC CACATGGGCC
661GTGGCCGGGA GGAAGCCATC ATGAAAATGG TGAAGCTGGA CCGGAAAGTG GGGCGCTCCT
721GCCAGCGCAT CGGGGAGGGG TGCTCCTGAA GGCCAGGCAT GGCCACCACG TGACGCTGTT
781CTTAGTTCAC TAATGTTAGC CTTATTTAGG ACAAAGTCAG CCAGACACCT TGTACTGGGC
841ACGCGTCAGA CTGCAGCCAG TCCGTTTCCT TTCTTTAGCC AGCCATCCTG GTACTGTAGT
901TTAGGGGTTG ATGGTGGTTG AAATTGATTT CTGGCTGGTT ACTAAGGTGC CTGCTAGCCA
961TTGTATAAAA TTAAAACATG AAGAATATTT TAAAAAAAAA AAAAAGGGCG GCCGCTCTAG
1021AGTATCCCTC GAGGGGCCCA AGCTTACGCG TACCCAGCTT TCTTGTACAA AGTGGTTGAT
1081ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT
1141CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAA
1201CTAAGGTCCT CACAGTTAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt
1261gtgaaagatt

Download FASTA (ORF) (Full)