Construct: ORF TRCN0000481287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005884.1_s317c1
- Derived from:
- ccsbBroadEn_03584
- DNA Barcode:
- CCCAGGGAGACTGACGTTACTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPSNAP3B (55335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481287
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NM_018376.4 | 100% | 100% | |
| 2 | human | 25934 | NIPSNAP3A | nipsnap homolog 3A | NM_015469.3 | 91% | 87% | (many diffs) |
| 3 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XM_011518839.1 | 79.7% | 79.7% | 428_429ins150 |
| 4 | human | 25934 | NIPSNAP3A | nipsnap homolog 3A | NM_001329570.2 | 73% | 69.2% | (many diffs) |
| 5 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_001746344.1 | 37.3% | 1_500del;1242_1984del | |
| 6 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NR_130760.2 | 37% | 1_112del;540_541ins150;704_1446del | |
| 7 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_001746345.1 | 35.8% | 1_500del;1079_1161del;1325_2067del | |
| 8 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_428532.1 | 12.3% | 1_500del;1079_1161del;1325_6018del | |
| 9 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NR_130759.2 | 9.5% | 1_112del;171_172ins211;643_5336del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cgttctcaga agcggcctga ccaaggcgct tgcctcacgg acgctcgcgc 121 ctcaggtgtg ttcatctttt gctacgggcc ctagacaata cgatggaacg ttctatgaat 181 ttcgtactta ttaccttaaa ccttcaaata tgaatgcgtt catggaaaat cttaagaaaa 241 acattcatct tcggacctct tactctgaat tggttggatt ctggagtgta gaatttggag 301 gcagaacgaa taaagtgttt catatttgga agtatgataa ttttgctcat cgagctgaag 361 ttcggaaagc cTTAGCCAAC TGTAAGGAAT GGCAAGAACA ATCTATCATT CCAAATTTGG 421 CTCGCATTGA TAAACAAGAG ACGGAAATTA CTTACCTGAT ACCATGGTCC AAATTAGAAA 481 AGCCTCCAAA AGAAGGAGTC TATGAACTAG CTGTTTTTCA GATGAAACCT GGTGGGCCAG 541 CTCTGTGGGG TGATGCATTT GAAAGAGCAA TTAATGCCCA TGTCAATTTA GGCTACACAA 601 AAGTAGTTGG TGTTTTCCAC ACAGAATATG GAGAACTCAA CAGAGTTCAT GTTCTTTGGT 661 GGAATGAGAG TGCAGATAGT CGTGCAGCTG GGAGACATAA GTCCCATGAG GATCCCAGAG 721 TTGTGGCGGC TGTTCGGGAA AGTGTCAACT ACCTAGTTTC TCAGCAGAAT ATGCTTCTGA 781 TTCCTGCATC ATTTTCACCA TTGAAAGACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCAGGGA 961 GACTGACGTT ACTGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt