Construct: ORF TRCN0000481294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000742.1_s317c1
- Derived from:
- ccsbBroadEn_13498
- DNA Barcode:
- GAGGTCCCAGATATCTCGGGACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASSF3 (283349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283349 | RASSF3 | Ras association domain fami... | XM_017019182.1 | 79.6% | 79.6% | 1_129del |
| 2 | human | 283349 | RASSF3 | Ras association domain fami... | XM_011538195.2 | 74.3% | 74.3% | 1_174del |
| 3 | human | 283349 | RASSF3 | Ras association domain fami... | XM_006719345.3 | 73.6% | 73.6% | 1_180del |
| 4 | human | 283349 | RASSF3 | Ras association domain fami... | NM_178169.4 | 70.5% | 70.5% | 1_210del |
| 5 | human | 283349 | RASSF3 | Ras association domain fami... | NR_040718.2 | 11.5% | (many diffs) | |
| 6 | mouse | 192678 | Rassf3 | Ras association (RalGDS/AF-... | XM_006513379.1 | 84.7% | 91.6% | (many diffs) |
| 7 | mouse | 192678 | Rassf3 | Ras association (RalGDS/AF-... | XM_011243388.1 | 63.5% | 68.7% | (many diffs) |
| 8 | mouse | 192678 | Rassf3 | Ras association (RalGDS/AF-... | NM_138956.3 | 60% | 64.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 573
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GACCTTGAAT TCAAATGGGA TTTACACTGG CTTCATTAAA GTACAGATGG 121 AACTCTGCAA ACCTCCACAG ACTTCTCCAA ATTCTGGAAA ACTCTCTCCC AGTAGCAATG 181 GCTGTATGAA TACACTTCAT ATCAGCAGCA CAAACACTGT CGGGGAAGTG ATCGAGGCCC 241 TGCTCAAAAA GTTTCTCGTG ACTGAGAGCC CTGCCAAGTT TGCACTTTAT AAGCGTTGTC 301 ACAGGGAAGA CCAAGTCTAC GCCTGCAAGC TCTCAGACCG GGAACATCCA CTCTACCTGC 361 GTTTGGTAGC AGGGCCCAGA ACAGACACAC TTAGTTTTGT TCTTCGTGAA CATGAAATTG 421 GAGAGTGGGA AGCCTTCAGC CTTCCAGAAC TACAGAATTT CTTGCGCATC TTGGACAAGG 481 AAGAAGATGA ACAGCTGCAG AACCTGAAGA GGCGCTACAC AGCCTACAGG CAGAAGCTGG 541 AAGAAGCCCT CCGTGAGGTG TGGAAGCCTG ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 721 GGTCCCAGAT ATCTCGGGAC ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt