Construct: ORF TRCN0000481297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017016.1_s317c1
- Derived from:
- ccsbBroadEn_10154
- DNA Barcode:
- CCTCCCTGTAGCTGTAGCCGACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND6B (414918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414918 | DENND6B | DENN domain containing 6B | NM_001001794.4 | 99.9% | 99.8% | 4G>T |
| 2 | human | 414918 | DENND6B | DENN domain containing 6B | XM_011530693.3 | 99% | 98.9% | 4G>T;1455_1469del |
| 3 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452244.1 | 95.8% | 95.7% | 4G>T;215_289del |
| 4 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452243.1 | 95% | 94.9% | 4G>T;215_289del;1530_1544del |
| 5 | human | 414918 | DENND6B | DENN domain containing 6B | XM_005261914.4 | 92.2% | 92.1% | 4G>T;452_598del |
| 6 | human | 414918 | DENND6B | DENN domain containing 6B | XM_011530692.3 | 91.4% | 91.3% | 4G>T;452_598del;1602_1616del |
| 7 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452245.1 | 90.4% | 91.5% | 4G>T;977_978ins52;1019_1145del |
| 8 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452242.1 | 83.8% | 84.7% | (many diffs) |
| 9 | human | 414918 | DENND6B | DENN domain containing 6B | XR_430477.4 | 80.7% | (many diffs) | |
| 10 | human | 414918 | DENND6B | DENN domain containing 6B | XR_001755246.2 | 78.7% | (many diffs) | |
| 11 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452246.1 | 64.6% | 64.6% | 0_1ins621 |
| 12 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452247.1 | 55.3% | 55.3% | 0_1ins783 |
| 13 | human | 414918 | DENND6B | DENN domain containing 6B | XM_024452248.1 | 55.3% | 55.3% | 0_1ins783 |
| 14 | mouse | 69440 | Dennd6b | DENN/MADD domain containing 6B | NM_027081.3 | 86% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cgcgctgttg ggcacagggc ctcgccgggc tcgcggctgc ctgggcgcgg 121 ctggacccac gtcttcaggt cgcgcggcgc ggaccccggc ggcgccctgg gcgcgcttct 181 ccgcctggct ggagtgtgtg tgcgtggtca ccttcgacct ggagctgggc caggcgctgg 241 agctggtgta tccgaacgac ttccggctca cagacaagga gaaaagcagc atctgctacc 301 tgtcttttcc cgactcgcac tcaggctgcc ttggagacac tcagttcagc ttccgcatgc 361 gccagtgtgg agggcagagg agcccctggc atgccgacga caggcactac aacagcaggg 421 cccctgtggc actgcagagg gagccggcac actacttcgg ctacgtgtac ttcaggcagg 481 tgaaggacag ctctgtgaag aggggctact tccagaagtc tttggtgctg gtgtcccgct 541 tgccctttgt ccggctgttc caagcgctgc taagcctcat cgcccccgag tactttgaca 601 agctggcgcc ctgcctggaa gcagtgtgca gtgagatcga ccagtggccg gcgcctgcac 661 ctgggcagac cctgaaccta cctgtcatgg gcgttgttgt ccaggtgcgc atcccatcca 721 gggtggacaa gtccgagtcc agtcctccga agcagtttga ccaagagaac ctgctgccag 781 ccccagtggt tcttgctagc gtccacgagc tggacctgtt caggtgcttc cggcctgtgc 841 tgactcatat gcagacactg tgggagctca tgctcctcgg ggagcccctg ctagtcctgg 901 caccctcgcc cgacgtgtcc tcggagatgg tgctggcctt gaccagctgc ctgcagcccc 961 tcaggttctg ctgcgacttc cgtccctact tcaccatcca tgacagcgag ttcaaggagt 1021 tcaccacacg cacgcaggcc ccaccaaacg tggtcctggg agtcacaaac cctttcttta 1081 tcaaaacact ccagcactgg ccccacatcc tccgagtcgg ggagcccaag atgtcaggag 1141 acctgcctaa gcaagtcaag ctgaaaaagc cttcaaggtt gaagaccctg gacaccaagc 1201 caggcctcta caccgcttac acggcccacc tccaccgcga caaggcgctg ctcaaacggc 1261 tgctcaaggg cgtgcagaag aagcggccgt cagatgtgca gagcgccctg ctgcggcggc 1321 acctcctgga gctcacccag agcttcatca tccccctgga gcactacatg gccagcctca 1381 tgccccTGCA GAAGAGCATC ACGCCCTGGA AGACTCCCCC CCAGATCCAG CCCTTCAGCC 1441 AGGATGACTT CCTGCGTAGC CTGGAGCATG CTGGGCCCCA GCTCACCTGC ATCCTCAAGG 1501 GCGACTGGCT GGGTCTCTAC AGGCGGTTTT TCAAGTCCCC CCATTTTGAT GGCTGGTACC 1561 GGCAGCGGCA CAAGGAGATG GCCCTGAAGC TGGAGGCCCT GCACCTGGAG GCTATCTGTG 1621 AGGCGAACAT CGAGACCTGG ATGAAAGACA AGTCCGAGGT GGAGGTCGTG GACCTGGTCC 1681 TGAAACTTCG TGAGAAGCTG GTGCGGGCTC AGGGCCACCA GCTCCCTGTG AAGGAGGCTA 1741 CGCTGCAGCG GGCACAGCTG TACATCGAGA CGGTCATCGG CTCCCTGCCC AAAGACCTGC 1801 AGGCTGTCTT GTGCCCTCCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCTC CCTGTAGCTG 1981 TAGCCGACGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt