Construct: ORF TRCN0000481324
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016541.2_s317c1
- Derived from:
- ccsbBroadEn_06715
- DNA Barcode:
- AAAACTTCAATTTAGTTGCAGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VIT (5212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481324
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5212 | VIT | vitrin | NM_001177970.2 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 5212 | VIT | vitrin | NM_001177971.2 | 97.3% | 96.3% | (many diffs) |
| 3 | human | 5212 | VIT | vitrin | NM_001177969.1 | 96.7% | 96.3% | (many diffs) |
| 4 | human | 5212 | VIT | vitrin | NM_001328661.2 | 96.2% | 94% | (many diffs) |
| 5 | human | 5212 | VIT | vitrin | NM_053276.4 | 94.6% | 94.2% | (many diffs) |
| 6 | human | 5212 | VIT | vitrin | XM_011532906.2 | 87.7% | 86.6% | (many diffs) |
| 7 | human | 5212 | VIT | vitrin | XM_017004326.2 | 86.7% | 84.6% | (many diffs) |
| 8 | human | 5212 | VIT | vitrin | XM_011532905.2 | 85.3% | 84.9% | (many diffs) |
| 9 | human | 5212 | VIT | vitrin | XM_017004327.1 | 68% | 67.6% | (many diffs) |
| 10 | human | 5212 | VIT | vitrin | XM_017004328.1 | 61.9% | 59.7% | (many diffs) |
| 11 | human | 5212 | VIT | vitrin | NM_001177972.2 | 26.9% | 25.1% | (many diffs) |
| 12 | mouse | 74199 | Vit | vitrin | XM_017317699.1 | 42.7% | 43% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2040
- ORF length:
- 1971
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggactgtt gttctcacta tgaaggcatc tgttattgaa atgttccttg 121 ttttgctggt gactggagta cattcaaaca aagaaacggc aaagaagatt aaaaggccca 181 agttcactgt gcctcagatc aactgcgatg tcaaagccgg aaagatcatc gatcctgagt 241 tcattgtgaa atgtccagca ggatgccaag accccaaata ccatgtttat ggcactgacg 301 tgtatgcatc ctactccagt gtgtgtggcg ctgccgtaca cagtggtgtg cttgataatt 361 caggagggaa aatacttgtt cggaaggttg ctggacagtc tggttacaaa gggagttatt 421 ccaacggtgt ccaatcgtta tccctaccac gatggagaga atcctttatc gtcttagaaa 481 gtaaacccaa aaagggtgta acctacccat cagctcttac atactcatca tcgaaaagtc 541 cagctgccca agcaggtgag accacaaaag cctatcagag gccacctatt ccagggacaa 601 ctgcacagcc ggtcactctg atgcagcttc tggctgtcac tgtagctgtg gccaccccca 661 ccaccttgcc aaggccatcc ccttctgctg cttctaccac cagcatcccc agaccacaat 721 cagtgggcca caggagccag gagatggatc tctggtccac tgccacctac acaagcagcc 781 aaaacaggcc cagagctgat ccaggtatcc aaaggcaaga tccttcagga gctgccttcc 841 agaaacctgt tggagcggat gtcagcctgg actgcaaaat tgacttgtcg tttttaattg 901 atgggagcac cagcattggc aaacggcgat tccgaatcca gaagcagctc ctggctgatg 961 ttgcccaagc tcttgacatt ggccctgccg gtccactgat gggtgttgtc cagtatggag 1021 acaaccctgc tactcacttt aacctcaaga cacacacgaa ctctcgagat ctgaagacag 1081 ccatagagaa aattactcag agaggaggac tttctaatgt aggtcgggcc atctcctttg 1141 tgaccaagaa cttcttttcc aaagccaatg gaaacagaag cggggctccc aatgtggtgg 1201 tggtgatggt ggatggctgg cccacggaca aagtggagga ggcttcaaga cttgcgagag 1261 agtcaggaat caacattttc ttcatcacca ttgaaggtgc tgctgaaaat gagaagcagt 1321 atgtggtgga gcccaacttt gcaaacaagg ccgtgtgcag aacaaacggc ttctactcgc 1381 tccacgtgca gagctggttt ggcctccaca agaccctgca gcctctggtg aagcgggtct 1441 gcgacactga ccgcctggcc tgcagcaaga cctgcttgaa ctcggctgac attggcttcg 1501 tcatcgacgg ctccagcagt gtggggacgg gcaacttccg caccgtcctc cagtttgtga 1561 ccaacctcac caaagagttt gagatttccg acacggacac gcgcatcggG GCCGTGCAGT 1621 ACACCTACGA ACAGCGGCTG GAGTTTGGGT TCGACAAGTA CAGCAGCAGG CCTGACATCA 1681 TCAACGCCAT CAAGAGGGTG GGCTACTGGA GTGGTGGCAC CAGCACGGGG GCTGCCATCA 1741 ACTTCGCCCT GGAGCAGCTC TTCAAGAAGT CCAAGCCCAA CAAGAGGAAG TTAATGATCC 1801 TCATCACCGA CGGGAGGTCC TACGACGACG TCCGGATCCC AGCCATGGCT GCCCATCTGA 1861 AGGGAGTGAT CACCTATGCG ATAGGCGTTG CCTGGGCTGC CCAAGAGGAG CTAGAAGTCA 1921 TTGCCACTCA CCCCGCCAGA GACCACTCCT TCTTTGTGGA CGAGTTTGAC AACCTCTATC 1981 AGTATGTCCC CAGGATCATC CAGAACATTT GTACAGAGTT CAACTCACAG CCTCGGAACT 2041 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2101 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2161 TTTATATATC TTGTGGAAAG GACGAAAAAC TTCAATTTAG TTGCAGACCA CGCGTTAAGT 2221 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt