Construct: ORF TRCN0000481325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016363.2_s317c1
- Derived from:
- ccsbBroadEn_09987
- DNA Barcode:
- TGCCGCAGGAAACGATTGATGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA1 (285848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_001145716.2 | 99.7% | 99.3% | 1010C>A;1211C>T;1306T>C |
| 2 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_173676.2 | 97.7% | 97.3% | (many diffs) |
| 3 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010778.1 | 91.5% | 91.1% | (many diffs) |
| 4 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_011514520.2 | 89.7% | 89.3% | (many diffs) |
| 5 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010777.2 | 82.2% | 81.8% | (many diffs) |
| 6 | human | 285848 | PNPLA1 | patatin like phospholipase ... | NM_001145717.1 | 80.5% | 80.2% | (many diffs) |
| 7 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010776.1 | 76.5% | 76.2% | (many diffs) |
| 8 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_011514519.2 | 75% | 74.6% | (many diffs) |
| 9 | human | 285848 | PNPLA1 | patatin like phospholipase ... | XM_017010779.1 | 72.8% | 72.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1407
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgcagatg atgaggcagt ttctgtaccg ggtcctgccc gaggactcct 121 acaaggtcac cacggggaag ctccatgtga gcctcacccg cttaacggac ggggagaatg 181 tggtggtttc agagttcacg tccaaggagg agctcattga ggccctatac tgcagctgct 241 tcgtcccggt gtactgtggc ctcatccccc cgacttaccg cggtgtgtgg gcatttctca 301 ctctgccccc acagaggtac atcgatgggg gcttcacggg catgcagccc tgtgccttct 361 ggaccgacgc catcaccatc tccaccttca gtgggcagca ggacatctgt ccccgggact 421 gcccggccat cttccacgac ttccgcatgt tcaactgctc cttccagttc tccctggaga 481 acatcgccag gatgacccac gcattgttcc ccccggacct ggtgatcctg cacgattact 541 actaccgagg gtacgaggat gcagttttgt acttgaggcg gctgaatgct gtttatctta 601 attcttcctc caagagagtg attttccccc gggtggaagt gtactgccag atagaactcg 661 cccttggcaa tgagtgccct gaacgcagtc aaccaagcct tcgagcacgg caggccagtc 721 tggaaggagc cacacaacct cacaaggagt gggttcccaa aggggatgga aggggcagcc 781 atggtccgcc tgtgtcccaa cctgtgcaga cacttgaatt cacatgcgag tcacctgttt 841 cagcaccagt ctctccactt gagcagccac ctgcacagcc actggcctct tcaactccac 901 tttctctaag tggcatgcca cctgtatcat tcccagctgt gcacaagcca cccagctcca 961 cacctggttc atcactgccc accccaccac ctggactgtc acctctgtca ccTCAGCAGC 1021 AGGTACAACC GTCTGGATCA CCAGCCAGAT CCCTACACTC TCAGGCACCC ACTTCACACA 1081 GGCCATCCCT GGGGCCTTCA ACTGTGGGGG CACCTCAAAC ACTGCCCCGA AGTTCTCTTT 1141 CAGCCTTCCC TGCTCAGCCA CCTGTGGAGG AACTAGGCCA AGAACAGCCC CAAGCTGTAG 1201 CTCTTCTTGT CTCTTCAAAA CCAAAAAGCG CCGTGCCTCT GGTTCATGTG AAGGAAACCG 1261 TCAGCAAGCC TTATGTAATG GAGAGCCCTG CTGAAGACTC AAACTGGGTG AATAAGGTCT 1321 TCAAGAAGAA CAAGCAAAAG ACAAGTGGCA CCAGAAAAGG CTTCCCAAGA CATCCGGGAT 1381 CCAAAAAACC AAGCAGCAAA GTGCAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1441 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1501 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGCCGCAG 1561 GAAACGATTG ATGACGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1621 aagatt