Construct: ORF TRCN0000481335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007821.1_s317c1
- Derived from:
- ccsbBroadEn_02410
- DNA Barcode:
- GGATATCGAACGTTACCCATTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICU1 (10367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481335
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | NM_001195518.2 | 100% | 100% | |
| 2 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | NM_006077.3 | 99.5% | 99.5% | 536_541delAGACAG |
| 3 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | NM_001363513.1 | 98.7% | 98.7% | 536_553del |
| 4 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | XM_011539119.1 | 89.4% | 89.4% | 534_701del |
| 5 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | XR_001746994.2 | 75.7% | (many diffs) | |
| 6 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | XR_945586.2 | 57.2% | 1_88del;1266_1385del;1637_2495del | |
| 7 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | NM_001195519.1 | 56.7% | 54.4% | (many diffs) |
| 8 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | XR_001746993.2 | 53.6% | (many diffs) | |
| 9 | human | 10367 | MICU1 | mitochondrial calcium uptake 1 | XM_005269386.2 | 52.1% | 52.1% | 0_1ins684 |
| 10 | mouse | 216001 | Micu1 | mitochondrial calcium uptake 1 | NM_144822.3 | 86.4% | 92.4% | (many diffs) |
| 11 | mouse | 216001 | Micu1 | mitochondrial calcium uptake 1 | XM_006513471.2 | 86.1% | 92% | (many diffs) |
| 12 | mouse | 216001 | Micu1 | mitochondrial calcium uptake 1 | NM_001291443.1 | 85.7% | 91.7% | (many diffs) |
| 13 | mouse | 216001 | Micu1 | mitochondrial calcium uptake 1 | NM_001291442.1 | 85.4% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcgtctgaac tcactttctg ctttggcaga actggctgtg ggttctcgat 121 ggtaccatgg aggatcacag cccatccaga tccggcgaag actaatgatg gtggctttcc 181 tgggagcatc tgcagtaact gcaagtactg gtcttttgtg gaagagggcc catgcagaat 241 ctccaccatg tgtagacaac ctaaaaagtg acatcggtga taaagggaag aataaagatg 301 aaggggatgt ttgtaaccat gagaaaaaga ctgcagatct tgcccctcac ccagaagaga 361 aaaagaagaa acgttctgga ttcagagaca gaaaagtgat ggaatatgag aataggattc 421 gagcctactc cacgccagac aaaatcttcc gatattttgc caccttgaaa gtcatcagtg 481 agcctggtga agcagaagtg tttatgacac cagaagattt tgtgcgatcc ataacaccca 541 atgaaaaaca accagaacac ttgggtctgg atcaatatat aataaaacgc tttgatggaa 601 agaaaatttc ccaggaacga gaaaaatttg ctgatgaagg cagtatattt tacacccttg 661 gagaatgtgg gctcatatcc ttttcagact acattttcct cacaactgtt ctttccactc 721 ctcagagaaa ttttgaaatt gccttcaaga tgtttgattt gaatggagat ggagaagtag 781 atatggaaga atttgaacag gttcagagca tcattcgctc ccaaaccagt atgggtatgc 841 gccacagaga tcgtccaact actggcaaca ccctcaagtc tggcttgtgt tcagccctca 901 caacctactt ttttggagct gatctgaagg gaaagctgac aatcaaaaac ttcctcgaat 961 ttcagcgtaa actgcagcat gatgttctga agcttgagtt tgaacgccat gaccctgtgg 1021 atgggagaat tactgagagg cagtttggtg gcatgctact tgccTACAGT GGGGTGCAGT 1081 CCAAGAAGCT GACCGCCATG CAGAGGCAGC TCAAGAAGCA CTTCAAAGAA GGAAAGGGTC 1141 TGACATTTCA GGAGGTGGAG AACTTCTTTA CTTTCCTAAA GAACATTAAT GATGTGGACA 1201 CTGCATTGAG TTTTTACCAT ATGGCTGGAG CATCTCTTGA TAAAGTGACC ATGCAGCAGG 1261 TGGCCAGGAC AGTGGCTAAA GTGGAGCTCT CAGACCACGT GTGTGATGTG GTGTTTGCAC 1321 TCTTTGACTG TGATGGCAAT GGCGAACTGA GCAATAAGGA ATTTGTTTCC ATCATGAAGC 1381 AACGGCTGAT GAGAGGCCTG GAAAAGCCCA AAGACATGGG TTTCACTCGC CTCATGCAGG 1441 CCATGTGGAA ATGTGCACAG GAAACTGCCT GGGACTTCGC TTTACCCAAA CAGTACCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAG GATATCGAAC GTTACCCATT GAGACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att