Transcript: Mouse NM_144822.3

Mus musculus mitochondrial calcium uptake 1 (Micu1), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Micu1 (216001)
Length:
2358
CDS:
127..1560

Additional Resources:

NCBI RefSeq record:
NM_144822.3
NBCI Gene record:
Micu1 (216001)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_144822.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000104658 CGCAAACTTCAGCATGACGTT pLKO.1 1033 CDS 100% 2.640 3.696 N Micu1 n/a
2 TRCN0000309487 CGCAAACTTCAGCATGACGTT pLKO_005 1033 CDS 100% 2.640 3.696 N Micu1 n/a
3 TRCN0000104659 ACAGACCAAACGAAGATTGAT pLKO.1 207 CDS 100% 5.625 3.938 N Micu1 n/a
4 TRCN0000104656 CCCTCAAGTCTGGCTTATGTT pLKO.1 938 CDS 100% 5.625 3.938 N Micu1 n/a
5 TRCN0000309417 CCCTCAAGTCTGGCTTATGTT pLKO_005 938 CDS 100% 5.625 3.938 N Micu1 n/a
6 TRCN0000104655 GCTGTGGATTTATAGTAGCAA pLKO.1 1894 3UTR 100% 3.000 2.100 N Micu1 n/a
7 TRCN0000309486 GCTGTGGATTTATAGTAGCAA pLKO_005 1894 3UTR 100% 3.000 2.100 N Micu1 n/a
8 TRCN0000104657 GCTCTGGATTCAGAGACAGAA pLKO.1 440 CDS 100% 0.495 0.347 N Micu1 n/a
9 TRCN0000309419 GCTCTGGATTCAGAGACAGAA pLKO_005 440 CDS 100% 0.495 0.347 N Micu1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144822.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02410 pDONR223 100% 86.4% 92.4% None (many diffs) n/a
2 ccsbBroad304_02410 pLX_304 0% 86.4% 92.4% V5 (many diffs) n/a
3 TRCN0000481335 GGATATCGAACGTTACCCATTGAG pLX_317 30.3% 86.4% 92.4% V5 (many diffs) n/a
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