Construct: ORF TRCN0000481360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006980.1_s317c1
- Derived from:
- ccsbBroadEn_15520
- DNA Barcode:
- TGCTTTGCTGAATTACAAGGGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDHA2 (5161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5161 | PDHA2 | pyruvate dehydrogenase E1 a... | NM_005390.5 | 100% | 100% | |
| 2 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_000284.4 | 83.1% | 85.1% | (many diffs) |
| 3 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173455.1 | 81.6% | 83.6% | (many diffs) |
| 4 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173454.1 | 76.5% | 77.6% | (many diffs) |
| 5 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | NM_001173456.1 | 76.5% | 78.2% | (many diffs) |
| 6 | human | 5160 | PDHA1 | pyruvate dehydrogenase E1 a... | XM_017029574.2 | 70.5% | 71.3% | (many diffs) |
| 7 | mouse | 18597 | Pdha1 | pyruvate dehydrogenase E1 a... | NM_008810.3 | 81% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1233
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggccgcc ttcatctccc gcgtgttgag gcgagttgcc cagaaatcag 121 ctcgcagagt gctggtggca tcccgtaact cctcaaatga cgctacattt gaaattaaga 181 aatgtgatct ttatctgttg gaagagggtc cccctgtcac tacagtgctc actagggcgg 241 aggggcttaa atactacagg atgatgctga ctgttcgccg catggaattg aaggcagatc 301 agctgtacaa acagaaattc attcgcggtt tctgtcacct gtgcgatggt caggaagctt 361 gttgcgtggg ccttgaggcc ggcataaacc cctcggatca cgtcattaca tcctataggg 421 ctcatggtgt gtgctatact cggggacttt ctgtccgatc cattctcgca gagctgacgg 481 gaagaagagg aggttgtgct aaaggaaaag gaggatcgat gcatatgtat accaagaact 541 tctatggggg caatggcatc gtcggtgcac agggccccct gggcgctggc attgctctgg 601 cctgtaaata taaaggaaac gatgagatct gtttgacttt atatggggat ggcgctgcga 661 atcaggggca gatagccgaa gctttcaata tggcagcttt atggaaatta ccttgtgttt 721 tcatctgtga gaataaccta tatggaatgg gaacatctac tgagagagca gcagccagcc 781 cTGATTACTA CAAGAGGGGC AATTTTATCC CTGGGCTAAA GGTCGATGGA ATGGATGTTC 841 TGTGTGTTCG TGAGGCAACA AAATTTGCAG CTAACTACTG TAGATCTGGA AAGGGGCCCA 901 TACTGATGGA GCTGCAAACC TACCGTTATC ATGGACACAG TATGAGTGAT CCTGGAGTCA 961 GTTATCGTAC ACGAGAAGAA ATTCAGGAAG TAAGAAGTAA GAGGGATCCT ATAATAATTC 1021 TCCAAGATAG AATGGTAAAC AGCAAGCTCG CCACTGTGGA AGAATTAAAG GAAATTGGGG 1081 CTGAGGTGAG GAAAGAAATT GATGATGCTG CCCAGTTTGC TACCACTGAT CCTGAGCCAC 1141 ATTTGGAAGA ATTAGGCCAT CACATCTACA GCAGTGATTC ATCTTTTGAA GTTCGTGGTG 1201 CAAATCCATG GATCAAGTTT AAGTCCGTCA GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1381 CTTTGCTGAA TTACAAGGGG CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt