Construct: ORF TRCN0000481363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006334.3_s317c1
- Derived from:
- ccsbBroadEn_15217
- DNA Barcode:
- CTTCCTGGCGGTTGGATTAAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STRADA (92335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_017025314.2 | 87.9% | 87.9% | 1_87del;664_672delCAGAATCTC |
| 2 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363791.1 | 80.6% | 79.2% | (many diffs) |
| 3 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257801.5 | 79.3% | 79.3% | 1_174del;751_759delCAGAATCTC |
| 4 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363790.1 | 76.5% | 75.3% | (many diffs) |
| 5 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363789.1 | 75% | 73.8% | (many diffs) |
| 6 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001165970.2 | 74.5% | 71% | (many diffs) |
| 7 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001165969.2 | 73.1% | 71.9% | (many diffs) |
| 8 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363788.1 | 66.9% | 65.8% | (many diffs) |
| 9 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_153335.6 | 66% | 64.9% | (many diffs) |
| 10 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003788.3 | 61.6% | 60.5% | (many diffs) |
| 11 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257799.3 | 61.6% | 60.5% | (many diffs) |
| 12 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_005257803.5 | 60.1% | 47.5% | (many diffs) |
| 13 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003786.3 | 58.3% | 57.3% | (many diffs) |
| 14 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_011525467.3 | 58% | 57% | (many diffs) |
| 15 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363787.1 | 57.1% | 56.2% | (many diffs) |
| 16 | human | 92335 | STRADA | STE20 related adaptor alpha | XM_011525466.3 | 57% | 56% | (many diffs) |
| 17 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001363786.1 | 54.3% | 53.4% | (many diffs) |
| 18 | human | 92335 | STRADA | STE20 related adaptor alpha | NM_001003787.4 | 53.3% | 52.4% | (many diffs) |
| 19 | human | 92335 | STRADA | STE20 related adaptor alpha | XR_243688.3 | 24.9% | (many diffs) | |
| 20 | human | 92335 | STRADA | STE20 related adaptor alpha | NR_156741.2 | 24.3% | (many diffs) | |
| 21 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534296.3 | 54.1% | 58.8% | (many diffs) |
| 22 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534297.3 | 54.1% | 58.8% | (many diffs) |
| 23 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_001252449.1 | 52.9% | 57.5% | (many diffs) |
| 24 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_028126.3 | 51.3% | 55.8% | (many diffs) |
| 25 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534295.1 | 51.3% | 55.8% | (many diffs) |
| 26 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534294.3 | 50.3% | 54.7% | (many diffs) |
| 27 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_006534293.3 | 47.8% | 52% | (many diffs) |
| 28 | mouse | 72149 | Strada | STE20-related kinase adapto... | NM_001252448.1 | 47% | 51% | (many diffs) |
| 29 | mouse | 72149 | Strada | STE20-related kinase adapto... | XM_011249270.1 | 47% | 51% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tagctttctg ccagagggag ggtgttacga gctgctcact gtgataggca 121 aaggatttga ggacctgatg actgtgaatc tagcaaggta caaaccaaca ggagagtacg 181 tgactgtacg gaggattaac ctagaagctt gttccaatga gatggtaaca ttcttgcagg 241 gcgagctgca tgtctccaaa ctcttcaacc aTCCCAATAT CGTGCCATAT CGAGCCACTT 301 TTATTGCAGA CAATGAGCTG TGGGTTGTCA CATCATTCAT GGCATACGGT TCTGCAAAAG 361 ATCTCATCTG TACACACTTC ATGGATGGCA TGAATGAGCT GGCGATTGCT TACATCCTGC 421 AGGGGGTGCT GAAGGCCCTC GACTACATCC ACCACATGGG ATATGTACAC AGGAGTGTCA 481 AAGCCAGCCA CATCCTGATC TCTGTGGATG GGAAGGTCTA CCTGTCTGGT TTGCGCAGCA 541 ACCTCAGCAT GATAAGCCAT GGGCAGCGGC AGCGAGTGGT CCACGATTTT CCCAAGTACA 601 GTGTCAAGGT TCTGCCGTGG CTCAGCCCCG AGGTCCTCCA GCAGGGTTAT GATGCCAAGT 661 CTGACATCTA CAGTGTGGGA ATCACAGCCT GTGAACTGGC CAACGGCCAT GTCCCCTTTA 721 AGGATATGCC TGCCACCCAG GGTGAAGACT CTACGGTAAA AGGAATTTGC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGACTTCCTG GCGGTTGGAT TAAAATAACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt