Construct: ORF TRCN0000481366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004844.1_s317c1
- Derived from:
- ccsbBroadEn_11910
- DNA Barcode:
- CACGTGGAATAGTAGCTCACGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM15B (29890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29890 | RBM15B | RNA binding motif protein 15B | NM_013286.5 | 63.2% | 63.1% | 1_981del;1778G>A |
| 2 | mouse | 109095 | Rbm15b | RNA binding motif protein 15B | NM_175402.4 | 55.9% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1758
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccgaggat gaccagcggg ccacgcgcaa cctcttcatt ggtaacctgg 121 accacagcgt atctgaggtg gagctgcgaa gggccttcga gaaatatggc atcatcgagg 181 aggtggtcat caagaggcct gcccgtggcc agggcggtgc ctatgccttc ctcaagttcc 241 agaacctgga catggcccat agggctaagg tggccatgtc gggccgagtg attggtcgca 301 accccattaa gataggctat ggcaaggcca accccaccac tcgtctctgg gtgggtggcc 361 tgggacctaa cacgtcactg gcggctctgg cccgagagtt tgaccgcttt gggagcattc 421 ggaccattga tcacgtcaaa ggagatagct ttgcctatat tcagtacgag agcttggacg 481 cagcccaggc cgcctgtgct aaaatgaggg gttttccctt gggtggacca gaccgcaggc 541 tccgcgtgga ttttgccaaa gcagaggaga ctcggtaccc ccagcagtac cagccctcgc 601 cactccctgt gcattatgag ctgctcacag atggatacac ccggcaccgc aacctggacg 661 ccgacctggt gcgggacagg acgcccccac accttctgta ctcagaccga gaccggactt 721 ttttggaagg ggactggacc agccccagta aaagctctga ccgccgaaac agccttgagg 781 gctacagtcg ctcagtgcgc agccggagtg gtgagcgttg gggggcagat ggagaccgtg 841 gtttgcccaa gccctgggaa gagaagcgga aacggagaag cctttccagt gaccgtggga 901 ggacaaccca ttcaccatat gaggaacgga gtaggaccaa gggcagtggg cagcagtcag 961 agcggggctc cgaccgcacc cctgagcgca gccgcaagga gaaccactcc agtgaaggga 1021 ccaaggagtc cagcagcaac tccctcagca acagcagaca tggggctgag gaacggggcc 1081 accaccacca ccaccacgag gctgcagact cttcccacgg gaagaaggca agagacagcg 1141 agcgcaatca ccggaccaca gaggccgagc ccaagcctct ggaagagcca aaacacgaga 1201 ccaaaaagct gaagaatctt tcagagtacg ctcagacact acagctgggt tggaatgggc 1261 ttctggtgtt gaaaaacagc tgcttcccca cgtctatgca tatccTAGAG GGGGACCAGG 1321 GGGTGATCAG CAGTCTCCTC AAAGACCACA CTTCTGGGAG CAAGCTGACC CAGCTGAAGA 1381 TCGCCCAGCG CCTTCGACTG GACCAGCCCA AGCTTGACGA GGTCACACGA CGCATCAAGC 1441 AGGGGAGCCC CAACGGCTAT GCGGTCCTCT TAGCCACCCA GGCAACCCCC AGTGGGCTTG 1501 GCACTGAGGG GATGCCCACA GTAGAGCCCG GTCTGCAGAG GCGGCTTCTC AGGAACCTGG 1561 TCTCCTACTT GAAACAGAAG CAGGCCGCAG GGGTGATCAG CTTGCCAGTG GGGGGGTCCA 1621 AGGGCAGAGA CGGCACAGGC ATGCTCTACG CCTTCCCACC CTGCGACTTT TCCCAGCAGT 1681 ACCTCCAGTC AGCACTAAGG ACATTGGGCA AGCTAGAAGA AGAACACATG GTGATAGTCA 1741 TCGTCAGAGA CACTGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACGTGG AATAGTAGCT 1921 CACGGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt