Construct: ORF TRCN0000481369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002642.1_s317c1
- Derived from:
- ccsbBroadEn_04845
- DNA Barcode:
- CTCCCAGGGCAAACTATTAATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D20 (128637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128637 | TBC1D20 | TBC1 domain family member 20 | NM_144628.4 | 100% | 100% | |
| 2 | human | 128637 | TBC1D20 | TBC1 domain family member 20 | XM_005260661.1 | 100% | 100% | |
| 3 | human | 128637 | TBC1D20 | TBC1 domain family member 20 | XM_006723540.3 | 84.6% | 84.6% | 67_68ins186 |
| 4 | human | 128637 | TBC1D20 | TBC1 domain family member 20 | XM_017027645.1 | 84.6% | 84.6% | 67_68ins186 |
| 5 | human | 128637 | TBC1D20 | TBC1 domain family member 20 | NR_111901.2 | 59.6% | 1_128del;1338_2026del | |
| 6 | mouse | 67231 | Tbc1d20 | TBC1 domain family, member 20 | NM_024196.3 | 88.8% | 93% | (many diffs) |
| 7 | mouse | 67231 | Tbc1d20 | TBC1 domain family, member 20 | XR_001783169.1 | 31.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctccggagt gcgcagggcg acggccccac ctccggccac tgggacggcg 121 gcgcggagaa ggcagacttt aacgccaaaa ggaaaaagaa agtggcagag atacaccagg 181 ctctgaacag tgatcccact gatgtggctg cccttagacg catggctatc agtgaaggag 241 ggctcctgac tgatgagatc agacgaaaag tgtggcccaa gctcctcaat gtcaatgcca 301 atgacccacc tcctatatca gggaagaacc tacggcagat gagcaaggac taccaacaag 361 tgttgctgga cgtccggcgg tcattgcggc ggttccctcc tggcatgcca gaggaacaga 421 gagaagggct ccaggaagaa ctgattgaca tcatcctcct catcttggag cgcaaccctc 481 agctgcacta ctaccagggc taccatgaca ttgtggtcac atttctgctg gtggtaggcg 541 agaggctggc aacatccctg gtagaaaaat tatctaccca ccacctcagg gattttatgg 601 atccaacaat ggacaacacc aagcatatat taaactatct gatgcccatc attgaccagg 661 tgaatccaga gctccatgac ttcatgcaga gtgctgaggt agggaccatc tttgccctca 721 gctggctcat cacctggttt gggcatgtcc tgtctgactt caggcacgtc gtgcggttat 781 atgacttctt cctggcctgc cacccactga tgccgattta ctttgcagcc gtgattgtgt 841 tgtatcgcga gcaggaagTC CTGGACTGTG ACTGTGACAT GGCCTCGGTC CACCACCTGT 901 TGTCCCAGAT CCCTCAGGAC TTGCCCTATG AGACACTGAT CAGCAGAGCA GGAGACCTTT 961 TTGTTCAGTT TCCCCCATCC GAACTTGCTC GGGAGGCCGC TGCCCAACAG CAAGCTGAGA 1021 GGACGGCAGC CTCTACTTTC AAAGACTTTG AGCTGGCATC AGCCCAGCAG AGGCCTGATA 1081 TGGTGCTGCG GCAGCGGTTT CGGGGACTTC TGCGGCCTGA AGATCGAACA AAAGATGTCC 1141 TGACCAAGCC AAGGACCAAC CGCTTTGTGA AATTGGCAGT GATGGGGCTG ACAGTGGCAC 1201 TTGGAGCGGC TGCACTGGCT GTGGTGAAAA GTGCCCTGGA ATGGGCCCCT AAGTTTCAGC 1261 TGCAGCTGTT TCCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTCCCAGGGC AAACTATTAA 1441 TGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt