Transcript: Human XM_017027645.1

PREDICTED: Homo sapiens TBC1 domain family member 20 (TBC1D20), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TBC1D20 (128637)
Length:
3286
CDS:
106..1131

Additional Resources:

NCBI RefSeq record:
XM_017027645.1
NBCI Gene record:
TBC1D20 (128637)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027645.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148575 CGTGCGGTTATATGACTTCTT pLKO.1 624 CDS 100% 4.950 6.930 N TBC1D20 n/a
2 TRCN0000278258 CGTGCGGTTATATGACTTCTT pLKO_005 624 CDS 100% 4.950 6.930 N TBC1D20 n/a
3 TRCN0000129790 CCTTCCTCTTACATCACATTA pLKO.1 1145 3UTR 100% 13.200 9.240 N TBC1D20 n/a
4 TRCN0000278257 CCTTCCTCTTACATCACATTA pLKO_005 1145 3UTR 100% 13.200 9.240 N TBC1D20 n/a
5 TRCN0000148772 CAATGGACAACACCAAGCATA pLKO.1 461 CDS 100% 4.950 3.465 N TBC1D20 n/a
6 TRCN0000278256 CAATGGACAACACCAAGCATA pLKO_005 461 CDS 100% 4.950 3.465 N TBC1D20 n/a
7 TRCN0000148910 CCATGACATTGTGGTCACATT pLKO.1 357 CDS 100% 4.950 3.465 N TBC1D20 n/a
8 TRCN0000130793 GATTTACTTTGCAGCCGTGAT pLKO.1 669 CDS 100% 4.050 2.835 N TBC1D20 n/a
9 TRCN0000297183 GATTTACTTTGCAGCCGTGAT pLKO_005 669 CDS 100% 4.050 2.835 N TBC1D20 n/a
10 TRCN0000130894 GCTTTGTGAAATTGGCAGTGA pLKO.1 1016 CDS 100% 2.640 1.848 N TBC1D20 n/a
11 TRCN0000318980 GCTTTGTGAAATTGGCAGTGA pLKO_005 1016 CDS 100% 2.640 1.848 N TBC1D20 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1893 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1893 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027645.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04845 pDONR223 100% 84.6% 84.6% None 67_68ins186 n/a
2 ccsbBroad304_04845 pLX_304 0% 84.6% 84.6% V5 67_68ins186 n/a
3 TRCN0000481369 CTCCCAGGGCAAACTATTAATGTC pLX_317 34.7% 84.6% 84.6% V5 67_68ins186 n/a
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