Transcript: Mouse NM_024196.3

Mus musculus TBC1 domain family, member 20 (Tbc1d20), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Tbc1d20 (67231)
Length:
1936
CDS:
103..1311

Additional Resources:

NCBI RefSeq record:
NM_024196.3
NBCI Gene record:
Tbc1d20 (67231)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_024196.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127101 GCACATCCTAAATTATCTGAT pLKO.1 657 CDS 100% 4.950 6.930 N Tbc1d20 n/a
2 TRCN0000325371 GCACATCCTAAATTATCTGAT pLKO_005 657 CDS 100% 4.950 6.930 N Tbc1d20 n/a
3 TRCN0000127103 CGTTGTGAGGTTATACGACTT pLKO.1 801 CDS 100% 4.050 5.670 N Tbc1d20 n/a
4 TRCN0000325301 CGTTGTGAGGTTATACGACTT pLKO_005 801 CDS 100% 4.050 5.670 N Tbc1d20 n/a
5 TRCN0000127100 CCACCCACTTATGCCCATTTA pLKO.1 834 CDS 100% 13.200 10.560 N Tbc1d20 n/a
6 TRCN0000325300 CCACCCACTTATGCCCATTTA pLKO_005 834 CDS 100% 13.200 10.560 N Tbc1d20 n/a
7 TRCN0000127102 CCATGACATCGTGGTCACATT pLKO.1 537 CDS 100% 4.950 3.960 N Tbc1d20 n/a
8 TRCN0000354046 CCATGACATCGTGGTCACATT pLKO_005 537 CDS 100% 4.950 3.960 N Tbc1d20 n/a
9 TRCN0000127099 CCATGTGTCTTGAGTATCTTT pLKO.1 1596 3UTR 100% 5.625 3.938 N Tbc1d20 n/a
10 TRCN0000148772 CAATGGACAACACCAAGCATA pLKO.1 641 CDS 100% 4.950 3.465 N TBC1D20 n/a
11 TRCN0000278256 CAATGGACAACACCAAGCATA pLKO_005 641 CDS 100% 4.950 3.465 N TBC1D20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024196.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04845 pDONR223 100% 88.8% 93% None (many diffs) n/a
2 ccsbBroad304_04845 pLX_304 0% 88.8% 93% V5 (many diffs) n/a
3 TRCN0000481369 CTCCCAGGGCAAACTATTAATGTC pLX_317 34.7% 88.8% 93% V5 (many diffs) n/a
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