Construct: ORF TRCN0000481388
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003641.1_s317c1
- Derived from:
- ccsbBroadEn_01987
- DNA Barcode:
- CTGACATGCAGATAACTTGGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAMP4 (8674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481388
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_003762.5 | 100% | 100% | |
| 2 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_001185127.2 | 99.2% | 99.2% | 113_114insAAG |
| 3 | human | 8674 | VAMP4 | vesicle associated membrane... | XM_017002713.2 | 70.9% | 52.7% | 217_218ins46;300_301ins77 |
| 4 | human | 8674 | VAMP4 | vesicle associated membrane... | NR_033704.2 | 15.8% | 1_205del;629_2675del | |
| 5 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_001347125.1 | 93.8% | 97.1% | (many diffs) |
| 6 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496934.2 | 93.8% | 97.1% | (many diffs) |
| 7 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_016796.3 | 89.7% | 92.9% | (many diffs) |
| 8 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496936.3 | 89.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 504
- ORF length:
- 423
- Sequence:
-
1 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT TGATCAACAA 61 GTTTGTACAA AAAAGTTGGC ATGCCTCCCA AGTTTAAGCG CCACCTCAAT GATGATGATG 121 TCACAGGTTC TGTGAAAAGT GAAAGGAGAA ATCTTTTGGA AGATGATTCA GATGAAGAAG 181 AGGACTTTTT TCTAAGGGGA CCATCTGGAC CAAGATTTGG ACCTAGAAAT GATAAAATTA 241 AGCATGTTCA GAATCAAGTG GATGAAGTTA TTGATGTCAT GCAAGAAAAT ATTACAAAGG 301 TAATTGAGAG AGGGGAGAGA CTAGATGAAC TACAGGACAA ATCAGAAAGC TTATCGGATA 361 ATGCAACAGC TTTTAGCAAC AGATCCAAAC AACTTCGAAG GCAAATGTGG TGGCGTGGAT 421 GCAAAATAAA AGCCATCATG GCTTTGGTTG CTGCTATCCT TTTGCTAGTG ATTATCATTC 481 TTATAGTCAT GAAATACCGT ACTTGCCCGA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGACATGCAG 661 ATAACTTGGT TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att