Construct: ORF TRCN0000481401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014540.1_s317c1
- Derived from:
- ccsbBroadEn_12444
- DNA Barcode:
- TAGTCACCGCCTACTTCAGGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLSTN2 (64084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64084 | CLSTN2 | calsyntenin 2 | NM_022131.3 | 99.8% | 99.7% | 2283C>T;2540_2541delTCinsAT;2837T>C |
| 2 | human | 64084 | CLSTN2 | calsyntenin 2 | XM_017007022.2 | 96.7% | 95.6% | (many diffs) |
| 3 | mouse | 64085 | Clstn2 | calsyntenin 2 | XM_006511334.3 | 88.9% | 94.1% | (many diffs) |
| 4 | mouse | 64085 | Clstn2 | calsyntenin 2 | NM_022319.2 | 87.1% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2934
- ORF length:
- 2865
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgcctggg cggctgtgct gggtgccgct cctgctggcg ctgggcgtgg 121 ggagcggcag cggcggtggc ggggacagcc ggcagcgccg cctcctcgcg gctaaagtca 181 ataagcacaa gccatggatc gagacttcat atcatggagt cataactgag aacaatgaca 241 cagtcatttt ggacccacca ctggtagccc tggataaaga tgcaccggtt ccttttgcag 301 gggaaatctg tgcgttcaag atccatggcc aggagctgcc ctttgaggct gtggtgctca 361 acaagacatc aggagagggc cggctccgtg ccaagagccc cattgactgt gagttgcaga 421 aggagtacac attcatcatc caggcctatg actgtggtgc tgggccccac gagacagcct 481 ggaaaaagtc acacaaggcc gtggtccata tacaggtgaa ggatgtcaac gagtttgctc 541 ccaccttcaa agagccagcc tacaaggctg ttgtgacgga gggcaagatc tatgacagca 601 ttctgcaggt ggaggccatt gacgaggact gctccccaca gtacagccag atctgcaact 661 atgaaatcgt caccacagat gtgccttttg ccatcgacag aaatggcaac atcaggaaca 721 ctgagaagct gagctatgac aaacaacacc agtatgagat cctggtgacc gcctacgact 781 gtggacagaa gcccgctgct caggacaccc tggtgcaggt ggatgtgaag ccagtttgca 841 agcctggctg gcaagactgg accaagagga ttgagtacca gcctggctcc gggagcatgc 901 ccctgttccc cagcatccac ctggagacgt gcgatggagc cgtgtcttcc ctccagatcg 961 tcacagagct gcagactaat tacattggga agggttgtga ccgggagacc tactctgaga 1021 aatcccttca aaagttatgt ggagcctcct ctggcatcat tgacctcttg ccatccccta 1081 gcgctgccac caactggact gcaggactgc tggtggacag cagtgagatg atcttcaagt 1141 ttgacggcag gcagggtgcc aaagtccccg atgggattgt gcccaagaac ctgaccgatc 1201 agttcaccat caccatgtgg atgaaacacg gccccagccc tggtgtgaga gccgagaagg 1261 aaaccatcct ctgcaactca gacaaaaccg aaatgaaccg gcatcactat gccctgtatg 1321 tgcacaactg ccgcctcgtc tttctcttgc ggaaggactt cgaccaggct gacacctttc 1381 gccccgcgga gttccactgg aagctggatc agatttgtga caaagagtgg cactactatg 1441 tcatcaatgt ggagtttcct gtggtaacct tatacatgga tggagcaaca tatgaaccat 1501 acctggtgac caacgactgg cccattcatc catctcacat agccatgcaa ctcacagtcg 1561 gcgcttgttg gcaaggagga gaagtcacca aaccacagtt tgctcagttc tttcatggaa 1621 gcctggccag tctcaccatc cgccctggca aaatggaaag ccagaaggtg atctcctgcc 1681 tgcaggcctg caaggaaggg ctggacatta attccttgga aagccttggc caaggaataa 1741 agtatcactt caacccctcg cagtccatcc tggtgatgga aggtgacgac attgggaaca 1801 ttaaccgtgc tctccagaaa gtctcctaca tcaactccag gcagttccca acggcgggtg 1861 tgcggcgcct caaagtatcc tccaaagtcc agtgctttgg ggaagacgta tgcatcagta 1921 tccctgaggt agatgcctat gtgatggtcc tccaggccat cgagccccgg atcaccctcc 1981 ggggcacaga ccacttctgg agacctgctg cccagtttga aagtgccagg ggagtgaccc 2041 tcttccctga tatcaagatt gtgagcacct tcgccaaaac cgaagccccc ggggacgtga 2101 aaaccacaga ccccaaatca gaagtcttag aggaaatgct tcataactta gatttctgtg 2161 acattttggt gatcggaggg gacttggacc caaggcagga gtgcttggag ctcaaccaca 2221 gtgagctcca ccaacgacac ctggatgcca ctaattctac tgcaggctac tccatctacg 2281 gtgtgggctc catgagccgc tatgagcagg tgctacatca catccgctac cgcaactggc 2341 gtccggcttc tcttgaggcc cggcgtttcc ggattaagtg ctcagaactc aatgggcgct 2401 acactagcaa tgagttcaac ttggaggtca gcatccttca tgaagaccaa gtctcagata 2461 aggagcatgt caatcatctg attgtgcagc ctcccttcct ccagtctgtc catcatcctG 2521 AGTCCCGGAG TAGCATCCAG CACAGTTCAG TGGTCCCAAG CATTGCCACA GTGGTCATCA 2581 TCATCTCCGT GTGCATGCTT GTGTTTGATG TGGCCATGGG TGTGTACCGG GTCCGGATCG 2641 CCCACCAGCA CTTCATCCAG GAGACTGAGG CTGCCAAGGA ATCTGAGATG GACTGGGACG 2701 ATTCTGCGCT GACTATCACA GTCAACCCCA TGGAGAAACA TGAAGGACCA GGGCATGGGG 2761 AAGATGAGAC TGAGGGAGAA GAGGAGGAAG AAGCCGAGGA AGAAATGAGC TCCAGCAGTG 2821 GCTCTGACGA CAGCGAAGAG GAGGAGGAGG AGGAAGGGAT GGGCAGAGGC AGACATGGGC 2881 AGAATGGAGC CAGGCAAGCC CAGCCGGAGT GGGATGACTC CACCCTCCCC TACTTGCCAA 2941 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3001 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3061 TATCTTGTGG AAAGGACGAT AGTCACCGCC TACTTCAGGT GGCACGCGTT AAGTCgacaa 3121 tcaacctctg gattacaaaa tttgtgaaag att