Construct: ORF TRCN0000481402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014471.2_s317c1
- Derived from:
- ccsbBroadEn_14987
- DNA Barcode:
- CAGCAATATACCGATGTCGAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K5 (11183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11183 | MAP4K5 | mitogen-activated protein k... | NM_006575.5 | 100% | 100% | |
| 2 | human | 11183 | MAP4K5 | mitogen-activated protein k... | NM_198794.3 | 100% | 100% | |
| 3 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_006720013.4 | 100% | 100% | |
| 4 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_024449461.1 | 100% | 100% | |
| 5 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536377.3 | 99.8% | 99.8% | 1703_1704insAGA |
| 6 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536378.3 | 92% | 92% | 0_1ins201 |
| 7 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536379.3 | 83.9% | 83.9% | 0_1ins408 |
| 8 | human | 11183 | MAP4K5 | mitogen-activated protein k... | XM_011536380.3 | 63.5% | 59.3% | (many diffs) |
| 9 | mouse | 399510 | Map4k5 | mitogen-activated protein k... | NM_201519.2 | 89.3% | 96.1% | (many diffs) |
| 10 | mouse | 399510 | Map4k5 | mitogen-activated protein k... | XM_006516054.3 | 89.2% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2604
- ORF length:
- 2538
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccccgctg cggcctgccg cggacatcct gaggcggaac ccgcagcagg 121 actacgaact cgtccagagg gtcggcagcg gcacctacgg ggacgtctat aaggccagaa 181 atgtacacac aggagagctg gctgcagtaa aaatcattaa attggagcct ggagatgatt 241 tttctttgat tcaacaagaa atatttatgg ttaaagaatg taaacattgt aacatcgttg 301 cctactttgg gagttatctt agtcgggaaa aactatggat ttgtatggaa tactgtggtg 361 gcggatcact tcaagatatt taccatgtta ctggaccatt atcagaattg caaatagcct 421 atgtatgcag agaaacctta cagggtcttg cctatttgca tactaaaggc aaaatgcata 481 gagatatcaa aggtgctaat attttattga cagaccatgg cgatgtaaaa ttagctgact 541 ttggtgtggc tgcaaaaata acagctacca ttgcaaaacg aaaatctttc attggcaccc 601 cttactggat ggccccagaa gttgcagcag tagagaagaa tggtggctac aaccaactct 661 gtgatatctg ggcagtagga ataacagcaa ttgaacttgg agaacttcag ccacctatgt 721 ttgatctcca cccaatgagg gctctcttct taatgtcaaa aagtaatttt cagcctccaa 781 aactaaagga caaaacaaaa tggtcatcaa cattccataa ttttgtcaaa atagcactaa 841 ccaaaaaccc aaaaaaaaga ccaactgctg aaagacttct gactcacact tttgttgcac 901 agccaggtct ctctagagcc ctagcagttg aactgttaga caaagtgaac aatccagata 961 accacgcaca ttacactgaa gcagatgacg atgactttga gccccatgca atcattcgtc 1021 ataccattag atctacaaac aggaatgcca gagctgaacg gacagcttca gaaataaatt 1081 ttgacaaatt acaatttgaa cctcctctga gaaaagaaac agaagcacga gatgaaatgg 1141 gattgtcatc agacccaaat ttcatgttac agtggaatcc ttttgttgat ggtgcaaata 1201 ctggcaaatc aacctcaaaa cgtgcaatac cacctcccct acctcctaag ccaaggataa 1261 gcagttaccc tgaagacaac tttccggatg aagaaaaagc atcaaccata aaacattgtc 1321 ctgattcaga aagcagagct ccccaaattc tcagaagaca gagtagccca agttgtgggc 1381 ctgtggcaga gacttcttct attggaaatg gtgatggtat ttcaaaactg atgagtgaaa 1441 atacagaagg atcagcacaa gcaccacagt taccacgaaa aaaggacaaa cgagacttcc 1501 ctaaaccagc catcaatggc cttccaccca ccccaaaagt tctgatggga gcatgctttt 1561 caaaagtttt tgatggctgt cctttgaaaa ttaattgtgc aacatcctgg atacatcctg 1621 atacaaaaga tcagtacatt atttttggaa ctgaagatgg tatttacaca ctgaatctca 1681 atgagctaca tgaggcaacg atggaacagt tatttccacg gaagtgtact tggctgtatg 1741 ttatcaataa tactttaatg tcattatcag aaggaaaaac ctttcagctc tactctcaca 1801 atcttatagc tttgtttgaa catgccaaaa aaccaggatt agctgcccat attcaaactc 1861 acaggtttcc agaccgaata ctaccaagaa aattcgcttt aacaacaaag attcctgata 1921 caaaaggctg ccacaaatgt tgcatagtca gaaaccctta cacgggacat aaatacctct 1981 gtggagcttt acagtctgga attgttttac ttcagtggta tgagccaatg cagaaattca 2041 tgttgataaa gcactttgat tttcctttgc caagtccttt gaatgttttt gaaatgctgg 2101 tgatacctga acaggaatac cctatggtct gtgtagctat tagcaaaggc actgaatcga 2161 atcaggtagt tcagtttgag acaatcaatt tgaactctgc aTCTTCATGG TTTACAGAAA 2221 TTGGTGCAGG CAGCCAGCAG TTAGATTCCA TTCATGTAAC ACAGTTGGAG AGAGATACCG 2281 TTTTAGTGTG TTTAGACAAA TTTGTGAAAA TTGTAAATCT ACAAGGAAAA TTAAAATCAA 2341 GTAAGAAACT GGCCTCTGAG TTAAGTTTTG ATTTTCGCAT TGAATCTGTA GTATGCCTTC 2401 AAGACAGTGT GTTGGCTTTC TGGAAACATG GGATGCAGGG TAAAAGCTTC AAGTCAGATG 2461 AGGTTACCCA GGAGATTTCA GATGAAACAA GAGTTTTCCG CTTATTAGGA TCAGACAGGG 2521 TTGTCGTTTT GGAAAGTAGG CCAACAGAAA ATCCTACTGC ACACAGCAAT CTCTACATCT 2581 TGGCTGGACA TGAAAATAGT TACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2641 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2701 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AGCAATATAC 2761 CGATGTCGAA CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2821 att