Construct: ORF TRCN0000481402

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014471.2_s317c1
Derived from:
ccsbBroadEn_14987
DNA Barcode:
CAGCAATATACCGATGTCGAACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP4K5 (11183)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481402
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11183 MAP4K5 mitogen-activated protein k... NM_006575.5 100% 100%
2 human 11183 MAP4K5 mitogen-activated protein k... NM_198794.3 100% 100%
3 human 11183 MAP4K5 mitogen-activated protein k... XM_006720013.4 100% 100%
4 human 11183 MAP4K5 mitogen-activated protein k... XM_024449461.1 100% 100%
5 human 11183 MAP4K5 mitogen-activated protein k... XM_011536377.3 99.8% 99.8% 1703_1704insAGA
6 human 11183 MAP4K5 mitogen-activated protein k... XM_011536378.3 92% 92% 0_1ins201
7 human 11183 MAP4K5 mitogen-activated protein k... XM_011536379.3 83.9% 83.9% 0_1ins408
8 human 11183 MAP4K5 mitogen-activated protein k... XM_011536380.3 63.5% 59.3% (many diffs)
9 mouse 399510 Map4k5 mitogen-activated protein k... NM_201519.2 89.3% 96.1% (many diffs)
10 mouse 399510 Map4k5 mitogen-activated protein k... XM_006516054.3 89.2% 95.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2604
ORF length:
2538
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggccccgctg cggcctgccg cggacatcct gaggcggaac ccgcagcagg
121actacgaact cgtccagagg gtcggcagcg gcacctacgg ggacgtctat aaggccagaa
181atgtacacac aggagagctg gctgcagtaa aaatcattaa attggagcct ggagatgatt
241tttctttgat tcaacaagaa atatttatgg ttaaagaatg taaacattgt aacatcgttg
301cctactttgg gagttatctt agtcgggaaa aactatggat ttgtatggaa tactgtggtg
361gcggatcact tcaagatatt taccatgtta ctggaccatt atcagaattg caaatagcct
421atgtatgcag agaaacctta cagggtcttg cctatttgca tactaaaggc aaaatgcata
481gagatatcaa aggtgctaat attttattga cagaccatgg cgatgtaaaa ttagctgact
541ttggtgtggc tgcaaaaata acagctacca ttgcaaaacg aaaatctttc attggcaccc
601cttactggat ggccccagaa gttgcagcag tagagaagaa tggtggctac aaccaactct
661gtgatatctg ggcagtagga ataacagcaa ttgaacttgg agaacttcag ccacctatgt
721ttgatctcca cccaatgagg gctctcttct taatgtcaaa aagtaatttt cagcctccaa
781aactaaagga caaaacaaaa tggtcatcaa cattccataa ttttgtcaaa atagcactaa
841ccaaaaaccc aaaaaaaaga ccaactgctg aaagacttct gactcacact tttgttgcac
901agccaggtct ctctagagcc ctagcagttg aactgttaga caaagtgaac aatccagata
961accacgcaca ttacactgaa gcagatgacg atgactttga gccccatgca atcattcgtc
1021ataccattag atctacaaac aggaatgcca gagctgaacg gacagcttca gaaataaatt
1081ttgacaaatt acaatttgaa cctcctctga gaaaagaaac agaagcacga gatgaaatgg
1141gattgtcatc agacccaaat ttcatgttac agtggaatcc ttttgttgat ggtgcaaata
1201ctggcaaatc aacctcaaaa cgtgcaatac cacctcccct acctcctaag ccaaggataa
1261gcagttaccc tgaagacaac tttccggatg aagaaaaagc atcaaccata aaacattgtc
1321ctgattcaga aagcagagct ccccaaattc tcagaagaca gagtagccca agttgtgggc
1381ctgtggcaga gacttcttct attggaaatg gtgatggtat ttcaaaactg atgagtgaaa
1441atacagaagg atcagcacaa gcaccacagt taccacgaaa aaaggacaaa cgagacttcc
1501ctaaaccagc catcaatggc cttccaccca ccccaaaagt tctgatggga gcatgctttt
1561caaaagtttt tgatggctgt cctttgaaaa ttaattgtgc aacatcctgg atacatcctg
1621atacaaaaga tcagtacatt atttttggaa ctgaagatgg tatttacaca ctgaatctca
1681atgagctaca tgaggcaacg atggaacagt tatttccacg gaagtgtact tggctgtatg
1741ttatcaataa tactttaatg tcattatcag aaggaaaaac ctttcagctc tactctcaca
1801atcttatagc tttgtttgaa catgccaaaa aaccaggatt agctgcccat attcaaactc
1861acaggtttcc agaccgaata ctaccaagaa aattcgcttt aacaacaaag attcctgata
1921caaaaggctg ccacaaatgt tgcatagtca gaaaccctta cacgggacat aaatacctct
1981gtggagcttt acagtctgga attgttttac ttcagtggta tgagccaatg cagaaattca
2041tgttgataaa gcactttgat tttcctttgc caagtccttt gaatgttttt gaaatgctgg
2101tgatacctga acaggaatac cctatggtct gtgtagctat tagcaaaggc actgaatcga
2161atcaggtagt tcagtttgag acaatcaatt tgaactctgc aTCTTCATGG TTTACAGAAA
2221TTGGTGCAGG CAGCCAGCAG TTAGATTCCA TTCATGTAAC ACAGTTGGAG AGAGATACCG
2281TTTTAGTGTG TTTAGACAAA TTTGTGAAAA TTGTAAATCT ACAAGGAAAA TTAAAATCAA
2341GTAAGAAACT GGCCTCTGAG TTAAGTTTTG ATTTTCGCAT TGAATCTGTA GTATGCCTTC
2401AAGACAGTGT GTTGGCTTTC TGGAAACATG GGATGCAGGG TAAAAGCTTC AAGTCAGATG
2461AGGTTACCCA GGAGATTTCA GATGAAACAA GAGTTTTCCG CTTATTAGGA TCAGACAGGG
2521TTGTCGTTTT GGAAAGTAGG CCAACAGAAA ATCCTACTGC ACACAGCAAT CTCTACATCT
2581TGGCTGGACA TGAAAATAGT TACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2641AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2701TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AGCAATATAC
2761CGATGTCGAA CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2821att

Download FASTA (ORF) (Full)