Construct: ORF TRCN0000481404
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012160.1_s317c1
- Derived from:
- ccsbBroadEn_11622
- DNA Barcode:
- AAGCACCGAATTTTGCACCAGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMC6 (11322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481404
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_011524257.3 | 39.4% | 39.4% | 1_273del;907_1605del |
| 2 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_024450557.1 | 36.5% | 36.5% | 1_402del;1036_1734del |
| 3 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_011524256.1 | 27.6% | 27.6% | 1_957del;1591_2289del |
| 4 | human | 11322 | TMC6 | transmembrane channel like 6 | NM_001127198.2 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 5 | human | 11322 | TMC6 | transmembrane channel like 6 | NM_001321185.1 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 6 | human | 11322 | TMC6 | transmembrane channel like 6 | NM_007267.7 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 7 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_005256995.1 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 8 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_011524255.1 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 9 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_024450555.1 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 10 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_024450556.1 | 26.2% | 26.2% | 1_1083del;1717_2415del |
| 11 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_017024107.1 | 25.6% | 20.8% | (many diffs) |
| 12 | human | 11322 | TMC6 | transmembrane channel like 6 | XM_017024108.1 | 25.6% | 20.8% | (many diffs) |
| 13 | human | 11322 | TMC6 | transmembrane channel like 6 | XR_243632.1 | 23.4% | 1_1271del;1905_2702del | |
| 14 | human | 11322 | TMC6 | transmembrane channel like 6 | XR_001752420.1 | 10% | 1_1271del;1905_6300del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcactctttc ggggagagct accgggtggg cagcacctct ggcatccacg 121 ccatcaccgt cttctgctcc tgggactaca aggtgacgca gaagcgggcc tcccgcctcc 181 agcaggacaa tattcgcacc cggctgaagg agctgctggc cgagtggcag ctgcggcaca 241 gccccaggag cgtgtgcggg aggctgcggc aggcggctgt gctggggctt gtgtggctgc 301 tgtgtctggg gaccgcgctg ggctgcgccg tggccgtcca cgtcttctcg gagttcatga 361 tccagagtcc agaggctgct ggccaggagg ctgtgctgct ggtcctgccc ctggtggttg 421 gcctcctcaa cctgggggcc ccctacctgt gccgtgtcct ggccgccctG GAGCCGCATG 481 ACTCCCCGGT ACTGGAGGTG TACGTGGCCA TCTGCAGGAA CCTCATCCTC AAGCTGGCCA 541 TCCTGGGGAC ACTGTGCTAC CACTGGCTGG GCCGCAGGGT GGGCGTCCTG CAGGGCCAGT 601 GCTGGGAGGA TTTTGTGGGC CAGGAGCTGT ACCGGTTCCT GGTGATGGAC TTCGTCCTCA 661 TGTTGCTGGA CACGCTTTTT GGGGAACTGG TGTGGAGGTG CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGAAAGCAC CGAATTTTGC ACCAGTCCAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt