Construct: ORF TRCN0000481423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015809.1_s317c1
- Derived from:
- ccsbBroadEn_01666
- DNA Barcode:
- ACACATGTGATGGCATCCCATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFBR1 (7046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_001130916.3 | 100% | 100% | |
| 2 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_004612.4 | 84.6% | 84.6% | 343_573del |
| 3 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_001306210.2 | 84% | 84% | 343_585del |
| 4 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518950.2 | 83.8% | 83.8% | 0_1ins207 |
| 5 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518949.2 | 70.9% | 70.9% | 0_1ins207;136_366del |
| 6 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_024447658.1 | 70.9% | 70.9% | 0_1ins207;136_366del |
| 7 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518948.2 | 70.4% | 70.4% | 0_1ins207;136_378del |
| 8 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_017015063.1 | 70.4% | 70.4% | 0_1ins207;136_378del |
| 9 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_001312868.1 | 76.8% | 81.9% | (many diffs) |
| 10 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_009370.3 | 76.2% | 81.2% | (many diffs) |
| 11 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_001312869.1 | 73.3% | 72.3% | (many diffs) |
| 12 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | XM_017320103.1 | 64.8% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcggcggtc gctgctccgc gtccccggct gctcctcctc gtgctggcgg 121 cggcggcggc ggcggcggcg gcgctgctcc cgggggcgac ggcgttacag tgtttctgcc 181 acctctgtac aaaagacaat tttacttgtg tgacagatgg gctctgcttt gtctctgtca 241 cagagaccac agacaaagtt atacacaaca gcatgtgtat agctgaaatt gacttaattc 301 ctcgagatag gccgtttgta tgtgcaccct cttcaaaaac tgggtctgtg actacaacat 361 attgctgcaa tcaggaccat tgcaataaaa tagaacttcc aactactggt ttaccattgc 421 ttgttcagag aacaattgcg agaactattg tgttacaaga aagcattggc aaaggtcgat 481 ttggagaagt ttggagagga aagtggcggg gagaagaagt tgctgttaag atattctcct 541 ctagagaaga acgttcgtgg ttccgtgagg cagagattta tcaaactgta atgttacgtc 601 atgaaaacat cctgggattt atagcagcag acaataaaga caatggtact tggactcagc 661 tctggttggt gtcagattat catgagcatg gatccctttt tgattactta aacagataca 721 cagttactgt ggaaggaatg ataaaacttg ctctgtccac ggcgagcggt cttgcccatc 781 ttcacatgga gattgttggt acccaaggaa agccagccat tgctcataga gatttgaaat 841 caaagaataT CTTGGTAAAG AAGAATGGAA CTTGCTGTAT TGCAGACTTA GGACTGGCAG 901 TAAGACATGA TTCAGCCACA GATACCATTG ATATTGCTCC AAACCACAGA GTGGGAACAA 961 AAAGGTACAT GGCCCCTGAA GTTCTCGATG ATTCCATAAA TATGAAACAT TTTGAATCCT 1021 TCAAACGTGC TGACATCTAT GCAATGGGCT TAGTATTCTG GGAAATTGCT CGACGATGTT 1081 CCATTGGTGG AATTCATGAA GATTACCAAC TGCCTTATTA TGATCTTGTA CCTTCTGACC 1141 CATCAGTTGA AGAAATGAGA AAAGTTGTTT GTGAACAGAA GTTAAGGCCA AATATCCCAA 1201 ACAGATGGCA GAGCTGTGAA GCCTTGAGAG TAATGGCTAA AATTATGAGA GAATGTTGGT 1261 ATGCCAATGG AGCAGCTAGG CTTACAGCAT TGCGGATTAA GAAAACATTA TCGCAACTCA 1321 GTCAACAGGA AGGCATCAAA ATGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CACATGTGAT 1501 GGCATCCCAT CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att