Transcript: Human XM_011518948.2

PREDICTED: Homo sapiens transforming growth factor beta receptor 1 (TGFBR1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TGFBR1 (7046)
Length:
2510
CDS:
240..1556

Additional Resources:

NCBI RefSeq record:
XM_011518948.2
NBCI Gene record:
TGFBR1 (7046)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144967 TAAAAGGGCGATCTAATGAA pXPR_003 GGG 299 23% 3 0.6272 TGFBR1 TGFBR1 76646
2 BRDN0001146778 AGAACGTTCGTGGTTCCGTG pXPR_003 AGG 535 41% 4 0.4564 TGFBR1 TGFBR1 76644
3 BRDN0001145243 ATGGGCAAGACCGCTCGCCG pXPR_003 TGG 733 56% 5 -0.7943 TGFBR1 TGFBR1 76645
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011518948.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221535 GCCTTGAGAGTAATGGCTAAA pLKO.1 1431 CDS 100% 10.800 15.120 N TGFBR1 n/a
2 TRCN0000221534 GCGAGAACTATTGTGTTACAA pLKO.1 648 CDS 100% 5.625 7.875 N TGFBR1 n/a
3 TRCN0000221537 CGATGTTCCATTGGTGGAATT pLKO.1 1284 CDS 100% 0.000 0.000 N TGFBR1 n/a
4 TRCN0000221533 GCTGGTCTTAACTTTAGGTAA pLKO.1 1839 3UTR 100% 4.950 3.960 N TGFBR1 n/a
5 TRCN0000195626 CCCTTCATTAGATCGCCCTTT pLKO.1 533 CDS 100% 4.050 3.240 N TGFBR1 n/a
6 TRCN0000194693 CTCATGTTGATGGTCTATATC pLKO.1 465 CDS 100% 13.200 9.240 N TGFBR1 n/a
7 TRCN0000196293 GAAGTTGCTGTTAAGATATTC pLKO.1 726 CDS 100% 13.200 9.240 N TGFBR1 n/a
8 TRCN0000221536 GCCACAGATACCATTGATATT pLKO.1 1125 CDS 100% 13.200 9.240 N TGFBR1 n/a
9 TRCN0000010441 CACAACAGCATGTGTATAGCT pLKO.1 231 5UTR 100% 3.000 2.100 N TGFBR1 n/a
10 TRCN0000010442 GAATGTTGGTATGCCAATGGA pLKO.1 1461 CDS 100% 3.000 2.100 N TGFBR1 n/a
11 TRCN0000010443 CAGTAAGTGCCACTTCTGTGT pLKO.1 2197 3UTR 100% 2.640 1.848 N TGFBR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011518948.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488036 ATTCCCATATCGAACTAAAGTGCA pLX_317 22.1% 85.6% 85.6% V5 (not translated due to prior stop codon) 0_1ins207;136_147del n/a
2 ccsbBroadEn_14861 pDONR223 100% 84.6% 29.8% None (many diffs) n/a
3 ccsbBroad304_14861 pLX_304 24.6% 84.6% 29.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000468797 GAGGTCCGTGGCCTGTTCCTGGGA pLX_317 25.8% 84.6% 29.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_01666 pDONR223 100% 70.4% 70.4% None 0_1ins207;136_378del n/a
6 ccsbBroad304_01666 pLX_304 52.7% 70.4% 70.4% V5 0_1ins207;136_378del n/a
7 TRCN0000481423 ACACATGTGATGGCATCCCATCCC pLX_317 38.9% 70.4% 70.4% V5 0_1ins207;136_378del n/a
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