Construct: ORF TRCN0000481462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016646.1_s317c1
- Derived from:
- ccsbBroadEn_08729
- DNA Barcode:
- CAGCTTTTCTACTGCCAAGTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYORG (57462)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57462 | MYORG | myogenesis regulating glyco... | NM_020702.5 | 99.9% | 99.7% | 11A>T;159C>G |
| 2 | human | 57462 | MYORG | myogenesis regulating glyco... | XM_011517966.3 | 99.9% | 99.7% | 11A>T;159C>G |
| 3 | human | 57462 | MYORG | myogenesis regulating glyco... | XM_017014930.2 | 99.9% | 99.7% | 11A>T;159C>G |
| 4 | mouse | 329828 | AI464131 | expressed sequence AI464131 | NM_001085515.2 | 83.8% | 91.6% | (many diffs) |
| 5 | mouse | 329828 | AI464131 | expressed sequence AI464131 | XM_006538010.2 | 83.8% | 91.6% | (many diffs) |
| 6 | mouse | 329828 | AI464131 | expressed sequence AI464131 | XR_881067.2 | 65.3% | (many diffs) | |
| 7 | mouse | 329828 | AI464131 | expressed sequence AI464131 | XR_881068.2 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2208
- ORF length:
- 2142
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ccagatccct caggagaaga gccaggccta cccccgccgc cgccggcctg 121 gctgctacgc ataccgtcag aaccccgagg ccatcgcagc cgcagctatg tacaccttcc 181 tgcccgacaa cttctcacct gccaagccca agccttccaa agagctgaag ccgctgctgg 241 gctccgcggt tctggggctg ctgcttgtgc tggccgcggt ggtggcctgg tgctactaca 301 gcgtctccct acgcaaggcg gagcgacttc gcgcggagct gctggacctg aaagctggcg 361 gcttctccat ccgcaatcag aagggagagc aggtcttccg cctggccttc cgctccggcg 421 cgctggacct tgactcctgc agccgcgatg gcgccctgct gggctgctcg ctcacggccg 481 acgggctgcc gctgcacttc ttcatccaga ctgtgcggcc caaggacacg gtcatgtgct 541 accgcgtgcg ctgggaggag gcagcgccgg gccgggccgt ggagcacgcc atgttcttgg 601 gcgacgcggc ggcccactgg tatggtggcg ccgagatgag gacgcaacac tggcccatcc 661 gcctggatgg ccagcaggag ccccagccgt tcgtcaccag cgatgtctac tcctccgacg 721 ccgcgtttgg gggcatcctc gagcgctact ggctatcttc gcgcgcggcc gccatcaaag 781 tcaatgactc agtgcccttc cacctgggct ggaacagcac ggagcgctcg ctgcggcttc 841 aggcgcgcta ccacgacacg ccctacaagc cacccgccgg ccgcgccgca gcgccagagc 901 tgagctaccg agtgtgcgtg ggctcagacg tcacctccat ccacaagtac atggtgcgtc 961 gctacttcaa caagccgtca agggtgccag cacccgaggc cttccgagac cccatttggt 1021 ccacatgggc gctgtacggg cgcgccgtgg accaggacaa ggtgctgcgt tttgcccaac 1081 agatccgcct gcaccacttc aacagcagcc acctggaaat cgacgacatg tacacacctg 1141 cttatggcga cttcgacttc gatgaggtca aattccccaa cgccagcgac atgttccgcc 1201 gcctgcgcga cgccggcttc cgcgtcacgc tctgggtgca cccttttgtc aactacaact 1261 cgtcgcgctt cggcgagggc gtggagcgcg agctgttcgt gcgcgaaccc acgggccggt 1321 tacctgcgct ggtgcgctgg tggaacggca tcggcgcggt gctagacttc acgcacccaa 1381 aggcccgcga ctggttccag ggacacctgc ggcggctgcg ctctcgctac tccgtggctt 1441 ccttcaagtt cgacgcgggc gaggtcagct acctgccgcg ggacttcagc acctaccggc 1501 cgctgccgga ccccagcgtc tggagccggc gctacactga gatggcgctg cccttcttct 1561 cgctggcgga ggtgcgcgta ggctaccagt cacagaacat ctcctgcttc ttccgcctgg 1621 tggatcgcga ctctgtgtgg ggctacgacc tggggttgcg ctcactcatc cccgcggtgc 1681 tcaccgTCAG CATGCTGGGC TACCCATTCA TCCTACCCGA TATGGTGGGC GGCAACGCCG 1741 TGCCCCAGCG GACAGCCGGC GGCGATGTGC CCGAGCGCGA GCTCTACATT CGCTGGCTGG 1801 AAGTGGCCGC CTTTATGCCG GCCATGCAGT TCTCTATCCC GCCCTGGCGC TACGACGCGG 1861 AAGTGGTGGC CATCGCGCAG AAGTTCGCCG CCCTGCGGGC CTCGCTTGTG GCACCGCTGT 1921 TGCTTGAGCT GGCGGGCGAG GTCACCGACA CGGGTGACCC TATCGTGCGC CCCCTTTGGT 1981 GGATTGCGCC CGGCGACGAG ACAGCTCACC GTATCGACTC GCAGTTCCTT ATTGGGGACA 2041 CGCTGCTTGT GGCCCCGGTG CTGGAGCCAG GCAAGCAGGA GCGCGACGTC TATTTGCCCG 2101 CCGGCAAGTG GCGCAGCTAC AAGGGTGAGC TTTTCGACAA GACGCCGGTG CTGCTCACCG 2161 ATTACCCGGT CGACCTGGAT GAGATCGCCT ACTTTACCTG GGCGTCCTGC CCAACTTTCT 2221 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2281 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2341 GTGGAAAGGA CGACAGCTTT TCTACTGCCA AGTCACGACG CGTTAAGTCg acaatcaacc 2401 tctggattac aaaatttgtg aaagatt