Construct: ORF TRCN0000481464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015233.1_s317c1
- Derived from:
- ccsbBroadEn_10638
- DNA Barcode:
- TTTCAGTACTAATAACAGGGATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CATSPER2P1 (440278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117155 | CATSPER2 | cation channel sperm associ... | NR_110319.1 | 11.9% | (many diffs) | |
| 2 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_001282309.3 | 11.5% | 8.6% | (many diffs) |
| 3 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_172095.4 | 11.5% | 8.6% | (many diffs) |
| 4 | human | 440278 | CATSPER2P1 | cation channel sperm associ... | NR_002318.2 | 11.4% | 1_256del;446_1649del | |
| 5 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_001282310.2 | 11.4% | 8.5% | (many diffs) |
| 6 | human | 110006325 | PPIP5K1P1-CATSPER2 | PPIP5K1P1-CATSPER2 readthrough | NR_146339.1 | 3.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 217
- ORF end:
- 406
- ORF length:
- 189
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGG CCGCTTACCA ACAAGAAGGG 241 CAGATGCAGC TTCCCCGAGC TGATGCCATT CGTTCACGTC TCATCGATAC TTTCTCTCTC 301 ATCGAGCATT TGCAAGGCTT GAGCCAAGCT GTGCTGCGAC ACACTATCAG GAGTTACTTG 361 ATCCTTCCCG CCAGAAGAAA CTTATGTTGG GAGATCAACA CCAGCTACCC AACTTTCTTG 421 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 481 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 541 GGAAAGGACG ATTTCAGTAC TAATAACAGG GATCTACGCG TTAAGTCgac aatcaacctc 601 tggattacaa aatttgtgaa agatt